The purification and some properties of rhodanese from tortoise (Kinixys erosa, Schweigger) liver

Aladesanmi, Omolara Titilayo; Okonji, Raphael Emuebie; Kuku, Adenike

doi:10.4314/ijbcs.v3i5.51053

Cited by 3 publications

(9 citation statements)

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“…Agboola and Okonji (2004) reported the inhibition of fruit bat rhodanese by Ba 2+ and Zn 2+ . This is also supported by the report of Aladesanmi et al (2009) . Inhibition by salts and certain monovalent anions may be due in part, to an increase in the ionic strength of the medium (Mintel and Westley, 1966).…”

Section: Resultssupporting

confidence: 84%

“…A pH range of 8.0 to 11.5 has been reported in various works (Chew and Boey, 1972;Lee et al, 1995;Agboola and Okonji, 2004;Okonji et al, 2010). The pH optimum of 8.5 was obtained for rhodanese from the liver of land tortoise (Aladesanmi et al, 2009). It has also been reported that at low pH (4 to 6) rhodanese is stabilized against inactivation (Kurban and Horowitz, 1991).…”

Section: Resultsmentioning

confidence: 81%

“…Different values of specific activities of rhodanese have been reported. Aladesanmi et al (2009) obtained 14.43 RU/mg for land tortoise liver rhodanese. Earlier, Sorbo (1953a, b) reported 256 RU/mg for bovine liver rhodanese.…”

Section: Resultsmentioning

confidence: 97%

“…The optimum temperature of 60°C for the P. brazzeana root rhodanese is similar to the results obtained by most researchers for rhodaneses from different sources ( Figure 6). Optimum temperature of 50°C was obtained for land tortoise liver rhodanese (Aladesanmi et al, 2009). Chew and Boey (1972) obtained optimum temperature of 59°C for cassava leaf rhodanese.…”

Section: Resultsmentioning

confidence: 99%

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Physicochemical properties of rhodanese: A cyanide detoxifying enzyme from Pentadiplandra brazzeana (Baill) root

Raphael¹,

Bamidele²,

Leonard³

et al. 2017

Afr. J. Biotechnol.

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Rhodanese a ubiquitous sulphur transferase enzyme catalyses the detoxification of cyanide to a less toxic thiocyanate. Rhodanese from the root of Pentadiplandra brazzeana was purified by ammonium sulphate precipitation and affinity chromatography. The enzyme had a specific activity of 4.82 RU/mg of protein. The K m values of the substrates (KCN and Na 2 S 2 O 3 ) were 11.76 and 10 mM, respectively. The optimum pH and temperature of the enzyme activity were 8.0 and 60°C, respectively. The enzyme was not inhibited by the heavy metals (BaCl 2 , KCl, NiCl 2 , NaCl, MnCl 2 , and ZnCl 2 ). This study affirmed the presence of rhodanese in the root of P. brazzeana, an indication that the plant may be of useful biotechnological application, especially in bioremediation of cyanide intoxified farm sites.

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Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 81%

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

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Physicochemical properties of rhodanese: A cyanide detoxifying enzyme from Pentadiplandra brazzeana (Baill) root

Raphael¹,

Bamidele²,

Leonard³

et al. 2017

Afr. J. Biotechnol.

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“…Rhodanese has been reported in many organisms from bacteria to animals (Agboola and okonji, 2004;Aladesanmi et al, 2009;Itakorode et al, 2019). In this study, we report the presence of rhodanese, a cyanide detoxifying enzyme in the kidney of grasscutter (T. swinderianus).…”

Section: Discussionmentioning

confidence: 99%

Physicochemical properties of rhodanese from the kidney of cane rat (Thryonomys swinderianus)

Ehigie¹,

Fagbohunka²,

Itakorode³

et al. 2019

Afr. J. Biotechnol.

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We report the properties of partially purified rhodanese, a cyanide detoxifying enzyme isolated from the kidney of the greater cane rat commonly known as grasscutter (Thryonomys swinderianus). T. swinderianus kidney rhodanese had a specific activity of 1.43 µmole/min/mg protein, a fold of 2.31 and a 55% recovery. The apparent K m values of 0.408 and 0.316 mM were obtained for both sodium thiosulphate (Na 2 S 2 O 3) and potassium cyanide (KCN) respectively. Substrate specificity study showed that T. swinderianus rhodanese can use other substrates. The enzyme showed its maximum activity at pH 8.0 and 50°C. The assay for the effect of metal ions showed that the enzyme is not affected or inhibited by metal ions such as Na + , Fe 3+ but inhibited by Hg 2+ . The continued existence of T. swinderianus after consumption of food crops with high cyanogenic glycosides suggests that the animal has a functional cyanide detoxification mechanism.

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Measurement of Sub-Lethal Toxicity and Effect of Kerosene Pollutant on Hematological Profile of African Catfish (Clarias gariepinus)

Ivon

Sanusi-Jadesola

Edu

et al. 2021

ARRB

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Background and Objectives: The study measured the impact of crude oil fractions and its derivatives like kerosene on the early life and sub-adults growth of African catfish, Clarias gariepinus in terms of mild, acute and chronic toxicity effect. It also examines the effect of indiscriminate spillage of crude oil derivatives on aquatic organisms. Materials and Methods: Blood profiles such as Red Blood Cell count (RBC), White Blood Cell count (WBC), Blood Differentials, Hemoglobin, (HB), and Packed Cell Volume (PCV) of the treated fishes and control were estimated after exposure of the fish to the kerosene pollutant. Behavioral changes in fish exposed to8.0ml/l, 16.0ml/l, 25.0ml/l and 50.0ml/l of kerosene pollutant varied from erratic swimming, moribund movement, jumping and lack of balance. Results: At concentration 8.0ml/L, mean total mortality was observed within 72hours of exposure. The lethal concentration (LC50) was 8.0ml/L and highest mean mortality observed with 80% kerosene pollutant. PCV of the exposed fish was 26% when compared to the control 27%, HB was 8.9 gdl as against 9.0 gdl in the control. Total WBC was 640 x 109/L as against 1280 x 109/L in control. RBC reading was 2.1 compared to 5.3 mm3 of the control. Lymphocyte was 80%, Neutrophil was 4% and monocyte 16% compared to 62%, 8% and 30% in control respectively. There was decrease in white blood cells counts for 8.0, 16.0, 25.0 and 50.0ml/L groups (p<0.05) compared to the control. Conclusion: The study concludes that it is necessary to ensure the safety of aquatic life forms especially fishes, by minimizing aquatic pollution with kerosene to sustain fish food quality, availability and security.

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The purification and some properties of rhodanese from tortoise (Kinixys erosa, Schweigger) liver

Cited by 3 publications

References 31 publications

Physicochemical properties of rhodanese: A cyanide detoxifying enzyme from Pentadiplandra brazzeana (Baill) root

Physicochemical properties of rhodanese: A cyanide detoxifying enzyme from Pentadiplandra brazzeana (Baill) root

Physicochemical properties of rhodanese from the kidney of cane rat (Thryonomys swinderianus)

Measurement of Sub-Lethal Toxicity and Effect of Kerosene Pollutant on Hematological Profile of African Catfish (Clarias gariepinus)

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