The purification of fatty acid methyl esters by high pressure liquid chromatography

Bascetta, Emanuele; Gunstone, Frank D.; Scrimgeour, Charles M.

doi:10.1007/bf02534474

Cited by 14 publications

(2 citation statements)

References 18 publications

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“…It was, therefore, necessary to use another step to obtain a fraction richer in 18:3 ~3. Preparative HPLC seemed to be a suitable method to isolate a pure fraction of 18:3 ~3, according to the results published by Bascetta et al (14). For this purpose, it was useful to carry out the HPLC fractionation on a fatty acid mixture which does not contain any saturated fatty acids, because these acids have large retention volumes and increase the quantity of solvent used.…”

Section: Resultsmentioning

confidence: 99%

Preparation of geometrical isomers of linolenic acid

Grandgirard

Julliard

Prevost

et al. 1987

J Americ Oil Chem Soc

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Two methods were developed to prepare important quantities of 18:3Δ9,12,15 geometrical isomers. The first method was based on the isolation of 18:3Δ9c,12c,15c from a linseed oil by a combination of urea inclusion and preparative high performance liquid chromatography (HPLC). The isolated 18:3 was isomerized using nitrous acid. The second method was based on the isolation of 18:3Δ9,12,15 geometrical isomers which usually are formed during the heat treatment of vegetable oils. For that purpose a linseed oil was heated at 275 C for 12 hr under nitrogen. The geometrical isomers were isolated using a combination of column chromatography on silicic acid, urea inclusion and preparative HPLC.

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Section: Resultsmentioning

confidence: 99%

Preparation of geometrical isomers of linolenic acid

Grandgirard

Julliard

Prevost

et al. 1987

J Americ Oil Chem Soc

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“…Preparative separations of FAME (100-200 µg in 10 µL) were carried out on two Shandon Hypersil C 18 columns (250 × 4 mm, 5 micron particles; Phase Separations Ltd., Macclesfield, United Kingdom) connected in series at ambient temperature. Methanol/water (90:10, vol/vol) at a flow rate of 1.0 mL/min was used as the mobile phase (22).…”

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confidence: 99%

Tanacetum (Chrysanthemum) corymbosum seed oil—A rich source of a novel conjugated acetylenic acid

Tsevegsüren

Christie²,

Lösel

1998

Lipids

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A new conjugated trans,trans-diunsaturated acetylenic acid (17%), found for the first time in nature as a main component of the seed oil of Tanacetum (Chrysanthemum) corymbosum, was shown to be octadeca-8t,10t-dien-12-ynoic acid. Another already known naturally occurring acetylenic acid, crepenynic acid (10%), was found also in the seed oil. The structure of this new unusual fatty acid was confirmed by chromatographic (thin-layer chromatography, gas chromatography, high-performance liquid chromatography) and spectroscopic (infrared, ultraviolet, mass spectrometry) methods by using different chemical derivatizations (deuteration, preparation of picolinyl ester, dimethyloxazoline, and maleic anhydride adduct).

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