The purification of four strains of poxvirus

Joklik, Wolfgang K.

doi:10.1016/0042-6822(62)90172-1

Cited by 538 publications

(278 citation statements)

References 30 publications

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“…Examination of purified suspensions by electron microscopy showed particles having the morphology of intact, mature poxvirus with little contaminating debris. Protein estimations gave values which were very close to those obtained by Joklik (1962) (Table 1) and the values obtained for the ratios of total/infective titres were similar to those obtained by previous workers (Table 1) (Dumbell, Downie & Valentine, 1957;Kaplan & Valentine, 1959;Joklik, 1962).…”

Section: Resultssupporting

confidence: 90%

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A comparison of the antigens present on the surface of virus released artificially from chick cells infected with vaccinia virus, and cowpox virus and its white pock mutant

Baxby

1972

J. Hyg.

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SUMMARYAntisera prepared against vaccinia and cowpox viruses were absorbed with purified suspensions of vaccinia virus, red cowpox and white cowpox viruses. They were then tested for their ability to neutralize the viruses, and to precipitate the virus soluble antigens.The results showed that some virus specific antigens were not virus surface components and that some components were present on the surface of all three viruses. However, certain components were detected on the surface of vaccinia virus but not on the surface of cowpox virus, and vice versa. Some evidence for the existence of a vaccinia-specific surface component was also obtained.Comparisons between results of cross-neutralization tests and immunodiffusion tests on the absorbed sera indicated that antibody to a number of antigens, including the classical LS, and the cowpox-specific d antigen play no part in the process of poxvirus neutralization.

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Section: Resultssupporting

confidence: 90%

“…Various preparations of soluble antigens were used, including some which contained only 1 or 2 specific line pattern components (lpc Joklik (1962).…”

Section: Testing Of Absorbed Antiseramentioning

confidence: 99%

A comparison of the antigens present on the surface of virus released artificially from chick cells infected with vaccinia virus, and cowpox virus and its white pock mutant

Baxby

1972

J. Hyg.

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“…Variola strains Butler (minor) and Harvey (major) were propagated in monolayer cultures of HeLa cells. Extraction and purification of viruses was essentially as described previously (Joklik, 1962;Archard & Mackett, I979) with the further modification that viruses were sedimented through columns of 36% sucrose in 5 raM-citrate-phosphate buffer (Mcllvain's), pH 7'4, overlaid with io% dextran T1o solution as described by Esposito et al 0978).…”

Section: Methodsmentioning

confidence: 99%

Conservation and Variation in Orthopoxvirus Genome Structure

Mackett¹,

Archard²

1979

Journal of General Virology

215

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“…The virus was recovered by centrifuging the diluted virus through a 36% sucrose cushion. The protein content of both viral preparations was calculated [12].…”

Section: Purification Of Virusesmentioning

confidence: 99%

Western blot analysis of virus-specific antibody responses for capripox and contagious pustular dermatitis viral infections in sheep

1994

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SUMMARYThis paper reports the development and evaluation of serological tests for the differentiation of antibodies in animals infected with capripox and parapox viruses. Agar-gel immunodiffusion tests using sera from sheep with naturallyacquired infections and from sheep experimentally inoculated with orf or capripox viruses showed cross reactions. Virus-specific antibody responses to structural proteins of the viruses were analysed by Western-blot analysis. This analysis readily differentiated the infections as either capripox or contagious pustular dermatitis. The antibody responses to the 32 kDa and 26 kDa proteins of capripoxvirus provided a firm basis for differention.

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The purification of four strains of poxvirus

Cited by 538 publications

References 30 publications

A comparison of the antigens present on the surface of virus released artificially from chick cells infected with vaccinia virus, and cowpox virus and its white pock mutant

A comparison of the antigens present on the surface of virus released artificially from chick cells infected with vaccinia virus, and cowpox virus and its white pock mutant

Conservation and Variation in Orthopoxvirus Genome Structure

Western blot analysis of virus-specific antibody responses for capripox and contagious pustular dermatitis viral infections in sheep

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