The purification of human polyclonal IgE by immunosorption

Aalberse, R. C.; Brummelhuis, H. G. J.; Reerink-Brongers, E.E.

doi:10.1016/0019-2791(73)90025-6

Cited by 23 publications

(10 citation statements)

References 11 publications

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“…Total IgE (detection limit 0.15 kU/l) in the neonates was determined at the Central Laboratory of the blood-transfusion services in Amsterdam. This was done in eluded blood spot material in a sandwich assay, according to Aalberse et al (20), with minor modifications: a mixture of anti-human IgE monoclonal antibodies was coupled to Sepharose 4B to catch IgE, and Sepharose-bound IgE was detected by means of radio-labeled antibodies against human IgE, raised in sheep.…”

Section: Sampling and Laboratory Analysesmentioning

confidence: 99%

Determinants of neonatal IgE level: parity, maternal age, birth season and perinatal essential fatty acid status in infants of atopic mothers

Gool

Thijs

Dagnelie

et al. 2004

Allergy

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Objective:The hygiene hypothesis suggests that the protective 'siblings effect' against atopic diseases such as atopic dermatitis, allergic asthma and hay fever is a result of recurrent infections during early childhood. A recent study and review have indicated that this protective effect may already arise in utero. Lower n-3 essential fatty acid (EFA) status is associated with increased parity, and EFA status has also been related to atopy. The present study confirms the negative association between parity and neonatal immunoglobulin E (IgE) levels and further unravel the role of perinatal EFA status. Methodology: In a prospective cohort study in 184 atopic mothers and their neonates, we simultaneously measured serum total IgE and EFA levels in plasma phospholipids, both in the mother at 34-36 weeks of gestation and in the neonate at the age of 1 week. Linear regression analysis was used to estimate the effect of parity on maternal and neonatal IgE and EFA status, and the independent effects of parity and EFA status on IgE, controlling for confounding factors such as maternal age and birth season. Results: Parity was associated with lower neonatal IgE level (P < 0.01), as well as with lower docosahexanoic acid (DHA, 22:6n-3) status of the mother (P = 0.01) but not of the neonate (P > 0.69). In the multivariate analysis, higher parity, higher maternal IgE, lower maternal age and birth in the first 3 months of the year were independently associated with neonatal IgE level. No association was detected between maternal or neonatal EFA status and neonatal IgE. Conclusions: As neonatal total serum IgE is predictive of later atopy, our results support the hypothesis that the sibling effect in atopy is already being programmed in utero. Our data also confirm earlier findings that DHA status is lower in multiparous women, but this did not confound the relation between parity and neonatal IgE.

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Section: Sampling and Laboratory Analysesmentioning

confidence: 99%

Determinants of neonatal IgE level: parity, maternal age, birth season and perinatal essential fatty acid status in infants of atopic mothers

Gool

Thijs

Dagnelie

et al. 2004

Allergy

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“…After absorption specificity of the absorbed antisera was again checked in the above tests. Antiserum against human IgG, specific in the hemagglutination technique, and fluorescent horse antiserum against rabbit immunoglobulins were the same as used by Capel [6], Specific antibodies for use in the IgG4-RAST were isolated as described by Aalberse et al [2] by absorption to and elution from an lgG4 myeloma protein cou pled to cellulose. Labelling with I25I was performed according to H unter and G reenwood [16].…”

Section: Methodsmentioning

confidence: 99%

Subclass Typing of IgG Antibodies Formed by Grass Pollen-Allergic Patients during Immunotherapy

M¹,

Wl²,

G³

et al. 1976

Int Arch Allergy Immunol

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131

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The subclasses of IgG antibodies formed by grass pollen-allergic patients during immunotherapy were investigated by using a radioallergosorbent test (RAST) and a quantitative immunofluorescence method known as the defined antigen substrate spheres (DASS) system. By the use of rabbit antisera directed against the subclasses of IgG, the specificity of which was checked in the passive hemagglutination and immunofluorescence techniques, it was shown that a relatively high proportion of the grass pollen-specific antibodies belonged to the IgG4 subclass. Apart from the high binding activity of IgG4 which increased during treatment, a moderate binding activity of the other subclasses was also found. Binding of all subclasses increased slightly in the pollen season and could be specifically blocked by preincubation with soluble grass pollen extract. The results of the IgG4 binding, determined in vitro with the DASS system, and the blocking activity of the sera, determined in vivo by skin tests are suggestive for a relation between these activities. Also in the group of patients with a low IgE-RAST score, the skin reactivity decreased as the IgG4 binding activity increased.

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“…A column was prepared and used to isolate anti-IgE antibodies from the sheep anti-IgE as described by Aalberse el ai. [1]. 30 tig purified antibody was reacted with 1 mC ,25I (Amersham) and 20 «g chloramine T.…”

Section: Assay Of Total Ige and Rast Preparation Of 2si-anti-igementioning

confidence: 99%

Estimation of Basophil-Bound IgE by Quantitative Immunofluorescence Microscopy

Stallman

Aalberse²

1977

Int Arch Allergy Immunol

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By means of a previously developed basophil staining and fixation technique, it was possible to identify human basophilic granulocytes in leukocyte suspensions, which had been subjected to an immunofluorescence technique with anti-IgE. Using fluoresceinated anti-IgE, the fluorescence intensity of the basophils was measured by means of micro-fluorometry as a reflection of the IgE-load per cell. The reproducibility of this technique and the influence of the incubation time were studied. The mean fluorescence intensity of the basophil varied considerably in six donors, but the cells of two atopic patients showed the highest intensity. IgE was eluted from the cells at pH 2.5 and measured in the supernatant. A correlation was found between the amount of eluted IgE per basophil and the mean fluorescence intensity of the basophils. The number of IgE molecules per basophil was found to be in the range of 15,000–500,000.

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The purification of human polyclonal IgE by immunosorption

Cited by 23 publications

References 11 publications

Determinants of neonatal IgE level: parity, maternal age, birth season and perinatal essential fatty acid status in infants of atopic mothers

Determinants of neonatal IgE level: parity, maternal age, birth season and perinatal essential fatty acid status in infants of atopic mothers

Subclass Typing of IgG Antibodies Formed by Grass Pollen-Allergic Patients during Immunotherapy

Estimation of Basophil-Bound IgE by Quantitative Immunofluorescence Microscopy

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