The putative C2H2 transcription factor RocA is a novel regulator of development and secondary metabolism in Aspergillus nidulans

Won, Dong Chan; Kim, Yong Jin; Kim, Da Hye; Park, Hee-Moon; Maeng, Pil Jae

doi:10.1007/s12275-020-0083-7

Cited by 4 publications

(3 citation statements)

References 34 publications

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“…Generally, most regulators are essential in the regulation of secondary metabolite production and the development of filamentous fungi due to their pleiotropic effects [ 24 , 27 ]. To establish the function of the CgXpp1 gene in the development of wild-type C. globosum W7, CgligD -N3, CgXpp1 -N14, and CgXpp1 -Com strains were cultured in a PDA medium at 28 °C under continuous light and dark conditions for 6 days.…”

Section: Resultsmentioning

confidence: 99%

Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in Chaetomium globosum

Zhao

Zhang

Lin

et al. 2022

IJMS

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Chaetoglobosin A (CheA), a well-known macrocyclic alkaloid with prominently highly antimycotic, antiparasitic, and antitumor properties, is mainly produced by Chaetomium globosum. However, a limited understanding of the transcriptional regulation of CheA biosynthesis has hampered its application and commercialization in agriculture and biomedicine. Here, a comprehensive study of the CgXpp1 gene, which encodes a basic helix-loop-helix family regulator with a putative role in the regulation of fungal growth and CheA biosynthesis, was performed by employing CgXpp1-disruption and CgXpp1-complementation strategies in the biocontrol species C. globosum. The results suggest that the CgXpp1 gene could be an indirect negative regulator in CheA production. Interestingly, knockout of CgXpp1 considerably increased the transcription levels of key genes and related regulatory factors associated with the CheA biosynthetic. Disruption of CgXpp1 led to a significant reduction in spore production and attenuation of cell development, which was consistent with metabolome analysis results. Taken together, an in-depth analysis of pleiotropic regulation influenced by transcription factors could provide insights into the unexplored metabolic mechanisms associated with primary and secondary metabolite production.

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Section: Resultsmentioning

confidence: 99%

Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in Chaetomium globosum

Zhao

Zhang

Lin

et al. 2022

IJMS

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“…Many genes negatively affect conidiation in A. nidulans 8 . Among these genes, nsdD and rocA encode putative transcription factors, and their deletions cause hyper-conidiation on agar media 37,38 . NsdD and RocA are thought to function upstream of brlA, which is the first transcription factor to function in the central regulatory cascade of A. nidulans conidiation [6][7][8] .…”

Section: Discussionmentioning

confidence: 99%

srdA mutations suppress the rseA/cpsA deletion mutant conidiation defect in Aspergillus nidulans

Ogawa

Fukuda

Iwama

et al. 2023

Sci Rep

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Conidiation is an important reproductive process in Aspergillus. We previously reported, in A. nidulans, that the deletion of a putative glycosyltransferase gene, rseA/cpsA, causes an increase in the production of extracellular hydrolases and a severe reduction in conidiation. The aim of this study was to obtain novel genetic factors involved in the repression of conidiation in the rseA deletion mutant. We isolated mutants in which the rseA deletion mutant conidiation defect is suppressed and performed a comparative genomic analysis of these mutants. A gene encoding a putative transcription factor was identified as the associated candidate causative gene. The candidate gene was designated as srdA (suppressor gene for the conidiation defect of the rseAdeletion mutant). The conidiation efficiency of the rseAsrdA double-deletion mutant was increased. Introduction of wild-type srdA into the suppressor mutants caused a conidiation defect similar to that of the rseA deletion mutant. Notably, the conidiation efficiencies of the rseAsrdA double-deletion and srdA single-deletion mutants were higher than that of the wild-type strain. These results indicate that srdA is a novel genetic factor that strongly represses conidiation of the rseA deletion mutant, and a putative transcriptional regulator, SrdA is a negative regulator of conidiation in A. nidulans.

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“…Among these families are the ERF family, intricately associated with ethylene signaling [53]; the C2H2 family, actively involved in developmental processes and secondary metabolism [54]; the IAA and ARF families, responsible for the response to growth hormones [55]; the DOF family, engaged in plant growth, metabolism, and hormone signaling [56]; and the YABBY family, which actively participates in morphogenesis, development, and phytohormone regulation [57]. In the TF-DEG network, it was observed that the YABBY, HD, C2H2, and ARF families collectively regulated 20 differentially expressed genes (DEGs), while the DOF, IAA, MYB-related, bZIP, and G2-like families collectively regulated 8 DEGs.…”

Section: Unveiling the Regulatory Network Of Pn Stem Apices By Analyz...mentioning

confidence: 99%

Unraveling Developmental Dynamics and Triterpene Saponin Biosynthesis in Panax notoginseng Stem Apices by Single-Cell Transcriptomics

Liu,

Yang,

Guo

et al. 2024

Preprint

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Background Panax notoginseng is often regarded as the foremost blood-nourishing herb due to its rich content of triterpene saponins, which possess various pharmacological activities. The transcriptional regulatory mechanisms governing stem apex development and the expression of its major component, triterpene saponins, remain poorly understood. Results In this study, we constructed a single-cell transcriptome atlas of stem apices, and the cells were divided into seven major cell types, comprising a total of 14 subclusters. Subsequently, we analyzed the developmental trajectory patterns and gene expression features of stem apices and inferred relevant transcription factors associated with stem apex development. We discovered potential key transcription factors, PTI5 and IDD12, involved in the early development of stem apices. Additionally, we investigated the expression and regulation of the triterpene saponin synthesis pathway in stem apices. Conclusions Therefore, the aim of this study is to elucidate the developmental trajectory of P. notoginseng stem apices and identify key transcription factors, opening a new avenue for future research of Panax genus.

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The putative C2H2 transcription factor RocA is a novel regulator of development and secondary metabolism in Aspergillus nidulans

Cited by 4 publications

References 34 publications

Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in Chaetomium globosum

Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production in Chaetomium globosum

srdA mutations suppress the rseA/cpsA deletion mutant conidiation defect in Aspergillus nidulans

Unraveling Developmental Dynamics and Triterpene Saponin Biosynthesis in Panax notoginseng Stem Apices by Single-Cell Transcriptomics

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