The Quail Mesonephros: A New Model for Renal Senescence?

Nacher, Víctor; Carretero, Ana; Navarro, Marc; Armengol, Clara; Llombart, Cristina; Rodríguez, Ángel Cervera; Herrero‐Fresneda, Immaculada; Ayuso, Eduard; Ruberte, Jesús

doi:10.1159/000096076

Cited by 37 publications

(35 citation statements)

References 40 publications

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“…In particular, in vitro approaches could be very useful to study whether endothelial cells in regressing vessels are per se more or less susceptible to transduction. In this study we examine primary cell cultures from quail mesonephros, a transitory embryonic kidney that undergoes growth and involution in a short period of time and shows progressive morphological changes throughout its life span, especially in the vascular system [Carretero et al, 1993[Carretero et al, , 1995[Carretero et al, , 1997Nacher et al, 2005Nacher et al, , 2006. The present study demonstrates that quail mesonephros is a suitable model in which to study endothelial cell transduction during vascular regression.…”

Section: Introductionmentioning

confidence: 83%

“…Embryo development was staged according to Hamburger and Hamilton [1951] (HH stages) and Zacchei [1961]. In accordance with our previous studies on quail embryos, we chose two stages in which mesonephros show striking vascular differences: 30HH (angiogenic mesonephric vasculature) and 40HH (regressing mesonephric vasculature) (6 and 11 days of incubation, respectively) [Nacher et al, 2005[Nacher et al, , 2006.…”

Section: Animal Samplesmentioning

confidence: 99%

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Endothelial Cell Transduction in Primary Cultures from Regressing Mesonephros

Nacher

Carretero

Navarro

et al. 2009

Cells Tissues Organs

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Loss of renal function during normal aging is associated with vascular alterations. Consequently, new therapeutic approaches, including gene therapy, to protect renal endothelial cells are expected to be greatly beneficial. Quail mesonephros is a transitory embryonic kidney that has been used for the study of vascular development and involution. Vascular alterations in regressing mesonephros are similar to those observed in aging kidney. In the present study, we examined adenovirus-mediated gene transfer to endothelial cells in primary cultures from developing and regressing quail mesonephros. Quail embryos with developing and regressing mesonephros were examined on day 6 (30HH) and day 11 (40HH) of incubation, respectively. The senescence markers, associated β-galactosidase activity and p16^INK4a, were examined in whole mesonephros. Quail embryos were injected intracardiacally with adenoviral vectors (rAd-CMV-LacZ) and endothelial cell transduction examined. In addition, primary cell cultures from mesonephros were exposed to adenoviral vectors. Endothelial cells in primary cultures were identified as QH1(+), LEP100(–) and acidic phosphatase(–) cells and adenovirus-transduced cells were those positive for bacterial-associated β-galactosidase activity. We report that endothelial cells in the whole regressing mesonephros and primary cell cultures expressed senescence markers. In addition, we observed that adenoviral vectors were able to transduce endothelial cells in the whole regressing mesonephros, and that cultured endothelial and macrophagic cells from the regressing mesonephros were more efficiently transduced than those derived from the developing mesonephros. Our results suggest that quail mesonephros provides a practical model to assay gene transfer to endothelial cells in regressing/senescent vessels.

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Section: Introductionmentioning

confidence: 83%

Section: Animal Samplesmentioning

confidence: 99%

Endothelial Cell Transduction in Primary Cultures from Regressing Mesonephros

Nacher

Carretero

Navarro

et al. 2009

Cells Tissues Organs

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“…Recent studies have shown that the molecular mechanisms characteristic of senescence also occur in a programmed manner during embryonic development (Nacher et al, 2006;Storer et al, 2013;Muñoz-Espin et al, 2013). There are not specific markers to define embryonic senescence processes but it is accepted that increased autophagy as detected by B-galactosidase activity, cell-cycle arrest, and matrix remodeling are indicators of physiological senescence.…”

Section: Interdigital Tissue Regression As a Potential Model Of Develmentioning

confidence: 99%

“…Of particular importance for the consolidation of the new hypothesis proposing the occurrence of an embryonic senescence process (Nacher et al, 2006;Storer et al, 2013;Muñoz-Espin et al, 2013) is the high expression of the transcription factor c-Rel in the regressing interdigits (Abbadie et al, 1993) and the presence of syndactyly in mice deficient in IKKa (Hu et al, 1999). The Rel/ NF-kB transcription factor family control cell-cycle, apoptosis, oxidative stress, immunity and inflammation and are considered central factors in aging and senescence (Gosselin and Abbadie, 2003).…”

Section: Transcription Factors and Mirnas Associated With Interdigitamentioning

confidence: 99%

Interdigital tissue regression in the developing limb of vertebrates

Lorda‐Diez¹,

Montero²,

García‐Porrero³

et al. 2015

Int. J. Dev. Biol.

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Here we have chosen the regression of the interdigital tissue which sculpts the digits from the hand/foot plate in tetrapod embryos to review the most relevant aspects concerning the regulation and biological significance of programmed cell death. We gather abundant information showing that the initiation of the degenerative process is the result of a complex interplay between the different signaling pathways which are also responsible for limb outgrowth and skeletal tissue differentiation, rather than being regulated by a specific signaling pathway. The model further shows that once the death response is triggered, several different routes of cell disruption, including caspase-dependent apoptosis, lysosomal-mediated cell death, and even a cell senescence process, are activated in the interdigits to ensure their elimination. Transcriptional and structural changes accompanying the degenerative process, and their posible contribution to the control of the death process, are also revised in detail. Finally we survey a number of issues still awaiting clarification, such as the functional implication of interdigital cell death as a source of signals acting on the surrounding tissues, as occurs in the so called "regenerative cell death".

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“…In the early fetal stage, the mesonephros regresses and the metanephros, i.e., the definitive kidney, starts to develop. The normal physiological regression of the mesonephros can serve as a model for the study of pathologically degenerating renal glomeruli because the morphological characteristics of developing mesonephric and metanephric glomeruli are highly similar (Gersh 1937;Leeson and Baxter 1957;Tiedemann and Egerer 1984;Smith and Mackay 1991;Nacher et al 2006Nacher et al ,2010. However, the molecular mechanisms of mesonephric glomerulogenesis are not well-known.…”

mentioning

confidence: 99%

Expression and Localization of Angiogenic Growth Factors in Developing Porcine Mesonephric Glomeruli

Spiegelaere

Cornillie

Erkens

et al. 2010

J Histochem Cytochem.

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S U M M A R Y The development and growth of renal glomeruli is regulated by specific angiogenic growth factors, including vascular endothelial growth factor (VEGF) and the angiopoietins (ANGPT1 and ANGPT2). The expression of these factors has already been studied during metanephric glomerulogenesis, but it remains to be elucidated during the development of the embryonic mesonephros, which can function as an interesting model for glomerular development and senescence. In this study, the presence of the angiogenic growth factors was studied in developing porcine mesonephroi, using IHC and real-time RT-qPCR on laser capture microdissected glomeruli. In addition, mesonephric glomerular growth was measured by using stereological methods. ANGPT2 remained upregulated during maturation of glomeruli, which may be explained by the continuous growth of the glomeruli, as observed by stereological examination. The mRNA for VEGFA was expressed in early developing and in maturing glomeruli. The VEGF receptor VEGFR1 was stably expressed during the whole lifespan of mesonephric glomeruli, whereas VEGFR2 mRNA was only upregulated in early glomerulogenesis, suggesting that VEGFR2 is important for the vascular growth but that VEGFR1 is important for the maintenance of endothelial fenestrations.

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The Quail Mesonephros: A New Model for Renal Senescence?

Cited by 37 publications

References 40 publications

Endothelial Cell Transduction in Primary Cultures from Regressing Mesonephros

Endothelial Cell Transduction in Primary Cultures from Regressing Mesonephros

Interdigital tissue regression in the developing limb of vertebrates

Expression and Localization of Angiogenic Growth Factors in Developing Porcine Mesonephric Glomeruli

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