In the fight against maternal and infant mortality, Senegal has defined screening tests guidelines for antenatal care. A minimal list of six (6) tests should be requested during the first ANC visit. To contribute to the reduction of maternal mortality in Senegal, we conducted a study to evaluate, this practice in health facilities, taking into account the national guidelines in order to formulate recommendations. The study was conducted in 16 health peripheral facilities. We collected data on tests requested, pregnant age, pregnancy age, gestity, midwives monthly salary, midwives number, type of structure (Hospital and Health Center), regions (capital and others), and the availability of guidelines. The quantitative data were collected from 1692 pregnant women, 61 midwives and 16 financial managers with appropriate tools, from February 2013 to July 2014. Data were entered in Microsoft Excel versions 2013 and Open Clinica and then exploited and analyzed in SPSS version 20. Univariate and bivariate analyses were computed. Only 49.2% of pregnant women have received the 6 recommended tests. Requested tests were Syphilis (96%), Blood group (90%), Emmel Test for sickle cell anemia (90%), Hemogram (81%), HIV serology (78%) and Protein urine test (67%). Factors associated with guidelines adherence were: (i) first antenatal visit (p <0.001), (ii) midwives monthly salary (R=-0,232), and (iii) regions type (p<0.001). It is important to take into account these results. For a better antenatal care, we recommend to train and motivate midwives on the national guidelines, in Senegal, and to sensitize childbearing about the importance of tests.