The quality of Gold Queen Hami melons stored under different temperatures

Ning, Ming; Tang, Fengxian; Zhang, Qin; Zhao, Xinxin; Yang, Liping; Cai, Wenchao; Shan, Chunhui

doi:10.1016/j.scienta.2018.08.005

“…Cantaloupe has a high sugar content and is susceptible to infection by pathogenic microorganisms [2], therefore, refrigerated storage is considered to be the most effective method for preserving the good quality of cantaloupe during long-distance transport [3]. However, cantaloupe is susceptible to cold damage during refrigeration, resulting in peel pitting and softening [4]. Use of cold-sensitive cultivars, longer storage times and lower temperatures are the major factors that contribute to the chilly injury.…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

Song

¹

,

Tang

²

,

Cai

³

et al. 2020

Preprint

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Background: Cantaloupe is susceptible to cold stress when it is stored at low temperatures, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of low temperatures resistance in cantaloupe, a cold-sensitive cultivar, Golden Empress-308 (GE) and a cold-tolerant cultivar, Jia Shi-310 (JS), were selected in parallel for iTRAQ quantitative proteomic analysis. Results: The two kinds of commercial cultivars were exposed to a temperature of 0.5℃ for 0, 12 and 24 days. We found that the cold-sensitive cultivar (GE) suffered more severe damage as the length of the cold treatment increased. Proteomic analysis of both cultivars indicated that the number of differentially expressed proteins (DEPs) changed remarkably during the chilly treatment. JS expressed cold-responsive proteins more rapidly and mobilized more groups of proteins than GE. Furthermore, metabolic analysis revealed that more amino acids were up-regulated in JS during the early phases of low temperatures stress. The DEPs we found were mainly related to carbohydrate and energy metabolism, structural proteins, reactive oxygen species scavenging, amino acids metabolism and signal transduction. The consequences of phenotype assays, metabolic analysis and q-PCR validation confirm the findings of the iTRAQ analysis. Conclusion: We found that the prompt response and mobilization of proteins in JS allowed it to maintain a higher level of cold tolerance than GE, and that the slower cold responses in GE may be a vital reason for the severe chilling injury commonly found in this cultivar. The candidate proteins we identified will form the basis of future studies and may improve our understanding of the mechanisms of cold tolerance in cantaloupe.

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“…Cantaloupe has a high sugar content and is susceptible to infection by pathogenic microorganisms [2], therefore, refrigerated storage is considered to be the most effective method for preserving the quality of cantaloupe during long-distance transport [3]. However, cantaloupe is susceptible to cold stress during refrigeration, resulting in peel pitting, softening, and weight loss [4].…”

Section: Introductionmentioning

confidence: 99%

“…To investigate how plants cope with abiotic stresses, previous studies have employed physiological and transcriptomics approaches [4,15]. Although transcriptome sequencing is a powerful method for identifying novel transcripts and analyzing gene expression at the transcriptional level, the changes in mRNA levels determined by transcriptomics do not always correlate with changes in the levels of the corresponding proteins [16].…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

Song

¹

,

Tang

²

,

Cai

³

et al. 2020

Preprint

Self Cite

0

View full text Add to dashboard Cite

Background: During the low temperature storage, cantaloupe is susceptible to the cold stress, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of cold tolerance in cantaloupe, cold-sensitive cultivar Goldqueen-308 (GE) and cold-tolerant cultivar Jiashi-310 (JS) were used for quantitative proteomic analysis with iTRAQ in parallel. Results: In this work, two commercial cultivars were treated at 0.5℃ for 0, 12 and 24 days. Phenotypes assays showed that GE suffered a more severe damage as the cold treatment time extended. Proteomic analysis revealed that the number of differentially expressed proteins (DEPs) changed significantly over time in cold-exposed cantaloupe. Comparing with GE, JS responded in a prompter manner in terms of expressing cold-responding proteins during the similarly cold treatment. Furthermore, much more different groups of proteins were mobilized in response to the cold treatment in JS comparing with GE. Metabolic analysis indicated that more amino acids were up-regulated in JS during the early phases of cold stress. This study also identified some DEPs since they were up-regulated in JS or down-regulated in GE in terms of molecular mechanisms, which were mainly related to carbohydrate and energy metabolism, structure proteins, ROS scavening, amino acid metabolic and signaling transduction. Moreover, iTRAQ analysis was confirmed to be reliable via the results of phenotypes assays, metabolic analysis and q-PCR validation. Conclusion: By proteomics information,we found that the prompt response and the significant mobilization of proteins in JS maintained a higher level of cold tolerance, and the delay of cold response in GE could be a critical reason for the severe chilling injury. The candidate proteins we found will be the basis of future studies for further investigations and our findings may help to better understand the novel mechanisms of cold tolerance in cantaloupe.

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“…Because of the high sugar content and susceptible to infection by pathogenic microorganisms [2], refrigerated storage is considered to be the most effective method for preserving the quality of cantaloupe, allowing long-distance transport [3]. During the low temperature storage, however, cantaloupe is susceptible to the cold stress, resulting in peel pitting, softening, and weight loss [4]. Longer storage time, more cold-sensitive cultivar and lower temperature are the major factors that easily cause the cold damage.…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

Song

¹

,

Tang

²

,

Cai

³

et al. 2020

Preprint

Self Cite

0

View full text Add to dashboard Cite

Background: During the low temperature storage, cantaloupe is susceptible to the cold stress, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of cold tolerance in cantaloupe, cold-sensitive cultivar Goldqueen-308 (GE) and cold-tolerant cultivar Jiashi-310 (JS) were used for quantitative proteomic analysis with iTRAQ in parallel. Results: In this work, two commercial cultivars were treated at 0.5℃ for 0, 12 and 24 days. Phenotypes assays showed that GE suffered a more severe damage as the cold treatment time extended. Proteomic analysis revealed that the number of differentially expressed proteins (DEPs) changed significantly over time in cold-exposed cantaloupe. Comparing with GE, JS responded in a prompter manner in terms of expressing cold-responding proteins during the similarly cold treatment. Furthermore, much more different groups of proteins were mobilized in response to the cold treatment in JS comparing with GE. Metabolic analysis indicated that more amino acids were up-regulated in JS during the early phases of cold stress. This study also identified some DEPs since they were up-regulated in JS or down-regulated in GE in terms of molecular mechanisms, which were mainly related to carbohydrate and energy metabolism, structure proteins, ROS scavening, amino acid metabolic and signaling transduction. Moreover, iTRAQ analysis was confirmed to be reliable via the results of phenotypes assays, metabolic analysis and q-PCR validation. Conclusion: By proteomics information,we found that the prompt response and the significant mobilization of proteins in JS maintained a higher level of cold tolerance, and the delay of cold response in GE could be a critical reason for the severe chilling injury. The candidate proteins we found will be the basis of future studies for further investigations and our findings may help to better understand the novel mechanisms of cold tolerance in cantaloupe.

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The quality of Gold Queen Hami melons stored under different temperatures

Cited by 21 publications

References 43 publications

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

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