The Quality of Platelet Concentrate Derived From Double Blood Collections by Trima Accel

Koike, Toshiyasu; Fuchizaki, Akihiro; Ichisugi, Megumi; Onodera, Hidekazu; Kaneko, Yuji; Iwama, Akira; Hirayama, Junichi; Shiba, Masayuki; Miyajima, Haruko; Hayashi, Yoshiaki; Arizawa, Fuminori; Fuse, Hisae; Naito, Yu; Wakamoto, Shinobu; Fujihara, Mitsuhiro; Chatani, Makoto; Kurihara, Katsuhiko; Mori, Jumpei; Terada, Akane; Ohashi, Yoshimi; Nagai, Tadashi; Satake, Masahiro

doi:10.3925/jjtc.64.490

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…This pore size allows fluid, cells, and cellular debris smaller than 0.8 μm in diameter to pass through the membrane to the filtrate port, while retaining platelets (Figure 1). To reduce the biochemical and physiological effects of polyvinyl chloride (PVC) on platelets, 17 immediately after the filtration step, the platelets were quickly transferred to a polyolefin bag (KBP‐1000FPN, Kawasumi Laboratories, Inc.). The WPC was then kept at room temperature for 30 min, followed by agitation for more than 30 min on a flatbed agitator (60 cycles/min) at 22°C.…”

Section: Methodsmentioning

confidence: 99%

Automated preparation of washed platelet concentrates through spinning‐membrane filtration

et al. 2022

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Background: Washed platelet concentrates (WPC), prepared with an automated system cell processor (ACP), have recently been approved to be manufactured and marketed in Japan. From the perspective of risk management, it is preferable to secure alternative technologies for ACP. Here, we conducted a study to evaluate the quality of WPC prepared using an automated membrane filtration-based system, Lovo.Study design and methods: Replaced PCs prepared from apheresis PCs were equally divided into control and test units, and subsequently washed using ACP and Lovo respectively. Work and operational efficiencies were evaluated by in vitro analyses, including total handling time, platelet recovery, and plasma protein removal rate. Product quality, including a set of biochemical and physiological indicators of platelets and supernatants, were assessed before and 3 days after washing. Results:In vitro platelet recovery rates and plasma protein removal rates were >85% and >95%, respectively, in both groups. The pH values on day 0 were significantly high (6.97 vs. 6.86) due to low pCO 2 in the test group, while no significant differences in glucose consumption and lactate production were observed between the two groups. The levels of hypotonic shock responses, aggregation response, platelet shape, CD62P expression, and sCD62P concentration were similar in both groups during the 3-day storage period. Conclusion:Platelet washing with Lovo provides platelet quality equivalent to, or better than, conventional washing with ACP. Thus, the new automated system, Lovo, can be considered as an alternative to ACP for WPC preparation.

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Section: Methodsmentioning

confidence: 99%

Automated preparation of washed platelet concentrates through spinning‐membrane filtration

et al. 2022

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Comparison of the Quality of Whole Blood-Derived, Pas-Replaced Platelet Concentrates During Room Temperature or Refrigerated Storage

Fujihara,

Kanashiki,

Fuse

et al. 2023

JJTC

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Pooled platelet concentrate (PC) that is prepared from whole blood may be a solution to the shortage of blood donors caused by the declining birthrate and aging population. Here, we prepared a pooled platelet additive solution (PAS)-PC from 400 ml whole blood by replacing a portion of the plasma with PAS using TACSI, a Europeanspecification large-capacity centrifuge with an automatic blood separation function. We then compared quality during storage at room temperature (22℃) and refrigeration (4℃) over 21 days.Pooling of four buffy coats (BCs) yielded more than 10 units of pooled PAS-PC (n=4), and pooling of five BCs yielded more than 15 units of pooled PAS-PC (n=4). Each pooled PAS-PC met the quality standard of PC in Japan. On room temperature storage, pooled PAS-PC maintained good platelet aggregation, % hypotonic shock response (%HSR), and clot formation capacity for up to 5 days. On refrigerated storage, %HSR decreased early after storage, but aggregation and clot formation capacity remained similar to those on day 5 of room temperature storage until day 14.These findings suggest that pooled PAS-PC of good quality can be obtained from 400 ml whole blood using the European specification TACSI. In addition, pooled PAS-PC can be stored for up to 14 days by refrigerated storage.

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The Quality of Platelet Concentrate Derived From Double Blood Collections by Trima Accel

Cited by 2 publications

References 13 publications

Automated preparation of washed platelet concentrates through spinning‐membrane filtration

Automated preparation of washed platelet concentrates through spinning‐membrane filtration

Comparison of the Quality of Whole Blood-Derived, Pas-Replaced Platelet Concentrates During Room Temperature or Refrigerated Storage

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