Purpose: The aim of this research was to determine the mental health status and quality of life of midwives and nurses working in primary health care services in Sivas city center. Methods: This research was done as a cross-sectional type. The universe of the research consists of a total of 187 nurses and midwives working in primary health care services in Sivas city center. In this study, no sample selection was made from the total population. In this study, we reached to 133 (71%) midwives and nurses who accepted to participate in the study. As data collection methods: Personal Information Form, the General Health Questionnaire-28 (GHQ-28) and the Turkish World Health Organization Quality of Life Questionnaire-Short Form were used. In the assessment, statistical tests including the significance test between the difference of two averages, analysis of variance, Tukey's test and chi-square test have been used and the margin of error was taken as 0.05. Results: The average age of midwives and nurses was 32.54±5.55. According to the General Health Questionnaire (GHQ-28), 21.1% of the employees were found to be at a high risk of having psychological problems. Employees' quality of life domain scores were found to be 16.53±2.16 for the physical area, 14.42±1.71 for the psychological area, 14.51±2.03 for the social area, 14.27±1.69 for the environmental area and 12.36±0.95 for the national area. Conclusion: According to these findings, general quality of life of the employees was found in the middle level, the average score for physical area at the highest level, and the average score for the national area at the lowest level. According to GSA-28, it is statistically significant that the life quality of employees who are considered as risky in terms of mental health. As a result, there was a significant positive relationship between mental health status, and quality of life of midwives and nurses. Employees who are mentally healthy, their quality of life was found to be better than the others.