The Quantification of Endolymphatic Hydrops in an Experimental Animal Model with Guinea Pigs

Chi, Fang‐Lu; Qin, Liang

doi:10.1159/000077795

Cited by 6 publications

(4 citation statements)

References 15 publications

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“…A high level EH was observed in the subapical turn, while the EH in the basal turn was markedly more moderate. These results agreed with those from a previous study by Chi and Liang [19] and indicated that the chronic EH model was successfully created.…”

Section: Resultssupporting

confidence: 92%

Effect of Endolymphatic Hydrops on Sound Transmission in Live Guinea Pigs Measured with a Laser Doppler Vibrometer

Ding

Wang

et al. 2016

Neural Plasticity

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Objective. This study aimed at describing the mechanism of hearing loss in low frequency and the different dynamic behavior of the umbo, the stapes head, and the round window membrane (RWM) between normal guinea pigs and those with endolymphatic hydrops (EH), using a laser Doppler vibrometer (LDV). Methods. Cochlear sections were stained with hematoxylin and eosin (HE) to evaluate the hydropic ratio (HR). Auditory brainstem responses (ABR) and whole-mount immunostaining were measured. Displacement of the umbo, stapes head, and RWM in response to ear-canal sound was evaluated using a LDV. Results. Mean HR values in EH model of all the turns are larger than the control group. The ABR threshold of the EH group was significantly higher than that of the control. Strong positive correlation was found between HR at apical turn and ABR threshold elevation at 1000 Hz and at subapical turn and ABR threshold elevation at 2000 Hz. FITC-phalloidin immunostaining of the cochlear basilar membrane in the apical, subapical, and suprabasal turns showed missing and derangement stereocilia of third-row outer hair cells. The umbo, stapes head, and RWM displacement in ears with EH was generally lower than that of normal ears. The EH-induced differences in stapes head and RWM motion were significant at 0.5 kHz. Conclusion. The LDV results suggested that the higher inner ear impedance in EH affected the dynamic behavior of the two opening windows of the cochlea and then reduced the vibration of the ossicular chain by increasing the afterload, resulting in acoustic dysfunction. The vibration reduction mainly occurred at low frequencies, which has related with the morphology changes of the apical and subapical turns in EH model.

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Section: Resultssupporting

confidence: 92%

Effect of Endolymphatic Hydrops on Sound Transmission in Live Guinea Pigs Measured with a Laser Doppler Vibrometer

Ding

Wang

et al. 2016

Neural Plasticity

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“…The animals were anesthetized with intramuscular ketamine hydrochloride (10 mg/kg) and xylazine (0.4 mg/kg). The approach to the endolymphatic sac was made through an extradural posterior cranial fossa [10,11,12]. The bone overlying the sigmoid sinus was sufficiently removed with an otologic drill after a skin incision was made near the dorsal midline.…”

Section: Methodsmentioning

confidence: 99%

Geometrical and Volume Changes of the Membranous Vestibular Labyrinth in Guinea Pigs with Endolymphatic Hydrops

Yang

Hua³

et al. 2013

ORL

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Objective: To evaluate geometrical and volume changes of membranous vestibular labyrinths in guinea pigs after endolymphatic hydrops (EH). Methods: The membranous labyrinths of normal guinea pigs and of those with EH for 4 and 8 weeks were reconstructed after being scanned using micro-computed tomography subseqent to being stained in osmium tetroxide (OsO₄). The diameters and volumes of the semicircular ducts, ampullae, utricles and saccules were measured based on the three-dimensional models. Results: The diameters of the ampullae and utricles of EH guinea pigs were greater than those of the normal guinea pigs, while there were no significant differences in the diameters of the semicircular ducts among all groups. The volumes of ampullae, utricles and saccules of the EH groups were greater than those of the control group, but there were no changes in volumes of semicircular ducts after EH. Conclusion: The dilations of the membranous vestibular labyrinth in guinea pigs with EH mainly occur at the ampullae, utricles and saccules.

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“…A limited amount of research has been performed on the application of automated image analysis for fish histopathology (e.g., Adams and Nowak, 2004;Fontagné et al, 2000;Galloway et al, 1999;Matos et al, 2013;Valente et al, 2011;Vatsos et al, 2010). Overall, few studies employing automated image analysis as a tool for fish histopathology exist, particularly when contrasted to the extensive use in other organisms of commercial, medical or veterinary importance (Ahrens et al, 1990;Anggraeni and Owens, 2000;Chi and Liang, 2004;De Laat et al, 2013;Dolapchieva et al, 2000;Vaughan et al, 1996;Veggetti et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Development of a quantitative semi-automated system for intestinal morphology assessment in Atlantic salmon, using image analysis

et al. 2015

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The Quantification of Endolymphatic Hydrops in an Experimental Animal Model with Guinea Pigs

Cited by 6 publications

References 15 publications

Effect of Endolymphatic Hydrops on Sound Transmission in Live Guinea Pigs Measured with a Laser Doppler Vibrometer

Effect of Endolymphatic Hydrops on Sound Transmission in Live Guinea Pigs Measured with a Laser Doppler Vibrometer

Geometrical and Volume Changes of the Membranous Vestibular Labyrinth in Guinea Pigs with Endolymphatic Hydrops

Development of a quantitative semi-automated system for intestinal morphology assessment in Atlantic salmon, using image analysis

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