Objective: The results of the determination of the phenolic compounds in Ruta graveolens L. and Stellaria media (L.) Vill. herb by the method of high-performance liquid chromatography (HPLC) with ultraviolet (UV)-detection are presented in the article.Method: The qualitative composition and quantitative content of the phenolic compounds were studied by HPLC on the Agilent 1200 chromatograph using methanolic extracts of R. graveolens L. and S. media (L.) Vill. herb. The components of hydroxycinnamic acids were determined at wavelengths of 320 nm and 330 nm; flavonoids at 255 nm and 340 nm; and tannins at 255 nm and 280 nm.Results: On the basis of the analysis conducted by the method of HPLC in the herb of R. graveolens L., 16 substances were identified: 6 flavonoids (apigenin, rutin, quercetin, luteolin, isoquercetin, and hyperoside), 4 hydroxycinnamic acids (chlorogenic, rosmarinic, caffeic, and p-coumaric), and 6 tannins (gallic and ellagic acids, gallocatechin, epigallocatechin, epicatechin, and epicatechin gallate). In the herb of S. media (L.) Vill., chlorogenic acid, flavone aglycones: Luteolin and apigenin, and flavonol glycosides: Isoquercetin and rutin were identified.Conclusion: According to the results of the research, it was established that the dominant components of R. graveolens L. and S. media (L.) Vill. herb are chlorogenic acid and a flavonoid component apigenin, which has anti-inflammatory activity. Thus, the obtained results point to the prospect of further research with the aim of creating of herbal substances with a certain pharmacological action.