The quantitative relationship of urinary peptide hydroxyproline excretion to collagen degradation

Weiss, P; Klein, L.

doi:10.1172/jci105957

Cited by 92 publications

(35 citation statements)

References 24 publications

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“…24-h urine samples were obtained from 21 untreated subjects with Paget's disease of bone. Five of these subjects were subsequently treated with the diphosphonate disodium etidronate (EHDP) (10) at doses of [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] …”

Section: Methodsmentioning

confidence: 99%

Urinary excretion of hydroxylysine and its glycosides as an index of collagen degradation.

Krane¹,

Kantrowitz²,

Byrne³

et al. 1977

J. Clin. Invest.

123

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A B S T R A C T Urinary excretion of hydroxyproline (Hyp) is one index of total collagen degradation, from all sources. Since some of the Hyp released from collagen may be further metabolized before it is excreted, other markers are necessary to measure collagen breakdown. Excretion of the glycosides of hydroxylysine (Hyl), glucosyl galactosyl hydroxylysine (Hyl[GlcGal ]), and galactosyl hydroxylysine (Hyl-[Gal]), more accurately reflects collagen metabolism since these products occur in specific ratios in different tissue collagens and are themselves metabolized only to a minor degree.The ratios of total Hyl/Hyp and Hyl(GlcGal)/Hyl-(Gal) were measured in the urine of normal subjects and of patients with Paget's disease of bone, hyperphosphatasia, and extensive thermal burns. In patients with extensive thermal bums the pattern of urinary Hyl and its glycosides was consistent with degradation of collagen in dermis and fascia. When sorption was decreased stufficiently with calcitonin or disodiuim etidronate in these patients, both the urinary ratios of Hyl/Hyp and Hyl(GlcGal)/Hyl(Gal) rose. In normal subjects treated with calcitonin and excreting relatively little Hyp, the ratio of Hyl/Hyp approached 0.7 and Hyl(GlycGal)/Hyl(Gal) approached 3.5. These increased ratios reveal the existence of a source of collagen breakdown other than skin or bone. The first subcomponent of complement, Cl(q, which has collagen-like sequences, relatively high amounts of Hyl, and most of the glycosylated Hyl as Hyl(GlcGal), could be the source of these metabolites.

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Section: Methodsmentioning

confidence: 99%

Urinary excretion of hydroxylysine and its glycosides as an index of collagen degradation.

Krane¹,

Kantrowitz²,

Byrne³

et al. 1977

J. Clin. Invest.

123

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“…Hydroxyproline is neither specific for bone (9-12) nor for bone resorption (10), moreover it is mostly metabolised before being excreted in urine (13). As a consequence hydroxyproline is a poor bone resorption marker (1).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Clinical Performances of the Immunoenzymometric Assays for N-Terminal and C-Terminal Type I Collagen Telopeptides and the HPLC Assay for Pyridinium Cross-Links

Bettica

Masino²,

Cucinotta³

et al. 1997

CCLM

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Summary:We evaluated the clinical performances of the immunoenzymometric assays for type I collagen Nterminal and C-terminal telopeptides and the HPLC assay for total deoxypyridinoline, in distinguishing between subjects with a moderately increased bone resorption rate (women in postmenopause) and subjects with normal bone resorption rate (women in premenopause). The postmenopausal group consisted of 61 women who had been in menopause for no more than 10 years, while the premenopausal group consisted of 52 healthy women with normal menstrual cycles. The biochemical markers were measured in a 24 hour urine sample and the results expressed as the molar ratio with urinary creatinine. The clinical performances were estimated by calculating the accuracy (as the area under a Receiver Operated Characteristic (ROC) curve: mean ± SEM) and the discriminating power (as score) of each assay in distinguishing postmenopausal subjects from premenopausal subjects. Type I collagen C-terminal telopeptide, type I collagen N-terminal telopeptide and total deoxypyridinoline were significantly higher in the postmenopausal than in the premenopausal group (p < 0.001). Accuracies of these three markers ranged from 66.8 ± 5.1% to 76.8 ± 4.3%, while Z scores ranged from 3.54 to 5.67. Type I collagen C-terminal telopeptide, type I collagen N-terminal telopeptide and total deoxypyridinoline were not significantly different in their accuracy or discriminating power. All markers were highly correlated with coefficients of correlation ranging from 0.61 to 0.77.In summary, this study shows that 1) the immunoenzymometric assays for type I collagen N-terminal telopeptide and type I collagen C-terminal telopeptide show a high accuracy and disciminating power in distinguishing subjects with different bone resorption rate;2) the results obtained with these immunoenzymometric assays are comparable to those obtained with the HPLC assay for total deoxypyridinoline.In conclusion our data support the use of the immunoenzymometric assays for type I collagen N-terminal telopeptide and type I collagen C-terminal telopeptide for estimating bone resorption. Introductiondinium cross-links were shown to be relatively efficient

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“…The organization of collagen may be altered in CP to create a stiffer ECM. Total collagen is measured by assaying for the exclusive collagen-specific modified amino acid hydroxyproline (10). Hydroxyproline is metabolized, excreted, and removed from the lung and kidney.…”

Section: Introductionmentioning

confidence: 99%

“…The other 30% is excreted via the urine. It has been suggested that the ratio of hydroxyproline excreted from the lung to that excreted from the urine is fairly constant; therefore, changes in collagen breakdown can be quantified by measuring the changes in the urine concentration of the prolyl-hydroxyproline peptide, which may reflect ineffective peptidase activity (10,11,12). However, no studies have investigated the correlations between the severity of clinical spasticity, Muscle Hydroxyproline (MH), and Urine Hydroxyproline (UH).…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that UH excretion is a sensitive indicator of collagen breakdown and can be used at the clinical level to predict changes in collagen metabolism (10). The aim of this study is to determine the correlation between the severity of clinical spasticity and UH and MH among children with CP who exhibit contracture or spasticity without contracture.…”

Section: Introductionmentioning

confidence: 99%

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Urine hydroxyproline correlates with progression of spasticity in cerebral palsy

Htwe

Lloyd

et al. 2017

Electron J Gen Med

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Background: Most Cerebral Palsy (CP) patients develop muscle spasticity which is characterized by jerky movements and muscle and joint stiffness. This increase of muscle stiffness in spastic CP has been correlated with the accumulation of collagen in the muscle as detected by the increase in muscle hydroxyproline, a major component of collagen. Objective: The objective of the study is to determine if there is any correlation between muscle and urine hydroxyproline levels in spastic CP. Further, to determine if Urine Hydroxyproline levels are different between spastic CP with and without contracture. Finally to determine if UH levels can be correlated with severity of CP as determined by Modified Ashworth Scale (MAS) and Gross Motor Function Classification System (GMFCS) scores. Methods: This was a cross sectional comparative study, conducted in the tertiary hospital, Malaysia from June'2012 to December'2014. Children with spastic CP (6 to 18 years) who were scheduled for muscle/tendon lengthening as part of the on going management and children with pure spasticity were included in this study. Normal children who are aged and sex matched to the CP children were included. Muscle biopsy and urine samples were collected for MH and UH analysis respectively. Results: A total of 48 children, aged 6 to 18 years (17 normal; 16 spastic CP without contracture, 15 spastic CP with contracture) were included in this study. Muscle biopsy (only for CP children with contracture) and urine samples were collected. A significant negative correlation was noted between the MH (261.894±69.077ng/ml) and UH (13.266±7.999ng/ml) levels (p=0.031). There was a statistically significant correlation between UH levels and the MAS score (p=0.01), and GMFCS score (p=0.015). Conclusion: UH quantification may be an objective tool to estimate the severity and progression of spasticity in CP.

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The quantitative relationship of urinary peptide hydroxyproline excretion to collagen degradation

Cited by 92 publications

References 24 publications

Urinary excretion of hydroxylysine and its glycosides as an index of collagen degradation.

Urinary excretion of hydroxylysine and its glycosides as an index of collagen degradation.

Comparison of the Clinical Performances of the Immunoenzymometric Assays for N-Terminal and C-Terminal Type I Collagen Telopeptides and the HPLC Assay for Pyridinium Cross-Links

Urine hydroxyproline correlates with progression of spasticity in cerebral palsy

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