The Queen Conch (Lobatus gigas) Proteome: A Valuable Tool for Biological Studies in Marine Gastropods

Domínguez-Pérez, Dany; Lippolis, John D.; Dennis, Michelle M.; Miller, Blake A.; Tiley, Katie; Vasconcelos, Vítor; Almeida, André M.; Campos, Alexandre

doi:10.1007/s10930-019-09857-0

Cited by 5 publications

(5 citation statements)

References 49 publications

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“…The Queen conch, Aliger gigas Linnaeus 1758 (Gastropoda, Strombidae; previously Strombus gigas Linnaeus, 1758; synonym Lobatus gigas [Linnaeus, 1758]), is restricted to coastal regions of the western Atlantic from Bermuda and southern Florida to Brazil 1 from 5–20 m in depth 2 . Aliger gigas is one of the largest gastropods in the world with a siphonal length up to 30 cm 3 , and has high ecological, esthetic, and economic value 4 .…”

Section: Introductionmentioning

confidence: 99%

The queen conch mitogenome: intra- and interspecific mitogenomic variability in Strombidae and phylogenetic considerations within the Hypsogastropoda

Machkour‐M’Rabet

Hanes

Martínez-Noguez³

et al. 2021

Sci Rep

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Aliger gigas is an economically important and vulnerable marine species. We present a new mitogenome of A. gigas from the Mexican Caribbean and use the eight publicly available Strombidae mitogenomes to analyze intra- and interspecific variation. We present the most complete phylogenomic understanding of Hypsogastropoda to date (17 superfamilies, 39 families, 85 genera, 109 species) to revisit the phylogenetic position of the Stromboidea and evaluate divergence times throughout the phylogeny. The A. gigas mitogenome comprises 15,460 bp including 13 PCGs, 22 tRNAs, and two rRNAs. Nucleotide diversity suggested divergence between the Mexican and Colombian lineages of A. gigas. Interspecific divergence showed high differentiation among Strombidae species and demonstrated a close relationship between A. gigas and Strombus pugilis, between Lambis lambis and Harpago chiragra, and among Tridentarius dentatus/Laevistrombus canarium/Ministrombus variabilis. At the intraspecific level, the gene showing the highest differentiation is ATP8 and the lowest is NAD4L, whereas at the interspecific level the NAD genes show the highest variation and the COX genes the lowest. Phylogenomic analyses confirm that Stromboidea belongs in the non-Latrogastropoda clade and includes Xenophoridea. The phylogenomic position of other superfamilies, including those of previously uncertain affiliation, is also discussed. Finally, our data indicated that Stromboidea diverged into two principal clades in the early Cretaceous while Strombidae diversified in the Paleocene, and lineage diversification within A. gigas took place in the Pleistocene.

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Section: Introductionmentioning

confidence: 99%

The queen conch mitogenome: intra- and interspecific mitogenomic variability in Strombidae and phylogenetic considerations within the Hypsogastropoda

Machkour‐M’Rabet

Hanes

Martínez-Noguez³

et al. 2021

Sci Rep

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“…Comparative transcriptomic, proteomic and metabolomic (i.e., multi-omics) techniques have proven critical to revealing the underlying molecular mechanisms that regulate hatching, growth, settlement and metamorphosis of commercially and scientifically important molluscan larvae [95], including Aplysia, Biomphalaria, Haliotis, Cornu, Lottia, Lymnaea and Aliger [225][226][227][228]. Proteomic, transcriptomic and associated expression profiling studies of A. gigas have provided significant insight into the reproductive mechanisms and genetic factors that underpin successful spawning in wild populations [226,229]. Furthermore, microsatellite analysis of Caribbean populations separated by 600 km found they were not panmictic (i.e., having limited gene flow) even though the veliger are capable of remaining in the plankton for up to two months and susceptible to environmental factors that promote larval dispersal.…”

Section: Octopus Cmentioning

confidence: 99%

A Review of the Giant Triton (Charonia tritonis), from Exploitation to Coral Reef Protector?

2022

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Charonia tritonis (Charoniidae), one of the largest marine gastropods and an echinoderm specialist, preys on Crown-of-Thorns starfish (CoTS), a recurring pest that continues to be a leading cause of coral mortality on Indo-Pacific reefs. Widespread historical exploitation has impacted their numbers, with standing populations considered rare throughout their habitat. Their life-stage attributes, i.e., teleplanic larvae, planktotrophic phase spanning years permitting transoceanic dispersal, and recruitment to coral reefs through oceanic influx with intense larval mortality, have likely hindered their recovery. Decline in numbers is hypothesised to account partially for periodic CoTS outbreaks, yet predator-prey dynamics between these two species that might influence this are poorly understood. The C. tritonis excretory secretome elicits a proximity deterrence effect on CoTS, the nature of which is under investigation as a possible tool in CoTS biocontrol scenarios. However, specificity and zone of impact in situ are unknown, and whether the mere presence of C. tritonis and/or predation pressure has any regulatory influence over CoTS populations remains to be established. The fundamental taxonomy and distinctive characteristics, biology and ecology of C. tritonis is summarized, and knowledge gaps relevant to understanding their role within coral reefs identified. Information is provided regarding exploitation of C. tritonis across its habitat, and prospects for conservation interventions, including captive rearing and stock enhancement to repopulate local regions, are discussed. Its predator-prey ecology is also examined and potential to mitigate CoTS considered. Recommendations to direct future research into this predator and for its inclusion in a CoTS integrated pest management strategy to improve coral reef health are offered.

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“…In such a context, either alone or combined, OMICs have been extensively used in a plethora of different subjects within life sciences. These include human health [ 29 ], animal sciences [ 30 ] and aquatic and environmental sciences [ 31 , 32 ]. Numerous reviews may be found in the subject and certain OMICs, namely proteomics, has been pointed out as being of particular relevance for animal, veterinary and aquatic science [ 33 , 34 , 35 ].…”

Section: Omics Overviewmentioning

confidence: 99%

“…Such a low level of representation in the databases can be a severe problem as it limits the amount of identified proteins, metabolites or transcripts. This problem is evident in farm animals [36] and aquatic species [37], where those with fully sequenced genomes such as the marine mussel (Mytilus galloprovincialis) [38] or oyster (Magallana gigas, old name Crassostrea gigas) [39] have better characterized proteome or transcriptome databases than other species like cockles (Ceratosderma edule), clams (Ruditapes decussatus) or the marine gastropod Lobatus gigas [31,40,41]. This problem is not as relevant in metabolomics as the metabolites are chemically very similar regardless of the studied organism.…”

Section: Omics Overviewmentioning

confidence: 99%

OMICs Approaches in Diarrhetic Shellfish Toxins Research

Campos

Freitas

Almeida

et al. 2020

Toxins

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Diarrhetic shellfish toxins (DSTs) are among the most prevalent marine toxins in Europe’s and in other temperate coastal regions. These toxins are produced by several dinoflagellate species; however, the contamination of the marine trophic chain is often attributed to species of the genus Dinophysis. This group of toxins, constituted by okadaic acid (OA) and analogous molecules (dinophysistoxins, DTXs), are highly harmful to humans, causing severe poisoning symptoms caused by the ingestion of contaminated seafood. Knowledge on the mode of action and toxicology of OA and the chemical characterization and accumulation of DSTs in seafood species (bivalves, gastropods and crustaceans) has significantly contributed to understand the impacts of these toxins in humans. Considerable information is however missing, particularly at the molecular and metabolic levels involving toxin uptake, distribution, compartmentalization and biotransformation and the interaction of DSTs with aquatic organisms. Recent contributions to the knowledge of DSTs arise from transcriptomics and proteomics research. Indeed, OMICs constitute a research field dedicated to the systematic analysis on the organisms’ metabolisms. The methodologies used in OMICs are also highly effective to identify critical metabolic pathways affecting the physiology of the organisms. In this review, we analyze the main contributions provided so far by OMICs to DSTs research and discuss the prospects of OMICs with regard to the DSTs toxicology and the significance of these toxins to public health, food safety and aquaculture.

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The Queen Conch (Lobatus gigas) Proteome: A Valuable Tool for Biological Studies in Marine Gastropods

Cited by 5 publications

References 49 publications

The queen conch mitogenome: intra- and interspecific mitogenomic variability in Strombidae and phylogenetic considerations within the Hypsogastropoda

The queen conch mitogenome: intra- and interspecific mitogenomic variability in Strombidae and phylogenetic considerations within the Hypsogastropoda

A Review of the Giant Triton (Charonia tritonis), from Exploitation to Coral Reef Protector?

OMICs Approaches in Diarrhetic Shellfish Toxins Research

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