Polysaccharides present in plant biomass, such as pectin, are the main carbon source for filamentous fungi.
Aspergillus niger
naturally secretes pectinases to degrade pectin and utilize the released monomers, mainly D‐galacturonic acid. The transcriptional activator GaaR, the repressor of D‐galacturonic acid utilization GaaX, and the physiological inducer 2‐keto‐3‐deoxy‐L‐galactonate play important roles in the transcriptional regulation of D‐galacturonic acid‐responsive genes, which include the genes encoding pectinases. In this study, we described the mutations found in
gaaX
and
gaaR
that enabled constitutive (i.e., inducer‐independent) expression of pectinases by
A. niger
. Using promoter‐reporter strains (P
pgaX
‐
amdS
) and polygalacturonic acid plate assays, we showed that W361R mutation in GaaR results in constitutive production of pectinases. Analysis of subcellular localization of C‐terminally
eGFP
‐tagged GaaR/Gaa
R
W
361R
revealed important differences in nuclear accumulation of N‐ versus C‐terminally
eGFP
‐tagged GaaR.