The R2R3-MYB transcription factor MiMYB1 regulates light dependent red coloration of ‘Irwin’ mango fruit skin

Kanzaki, Sho; Ichihi, Asuka; Tanaka, Yuta; Fujishige, Shiina; Koeda, Sota; Shimizu, Kosuke

doi:10.1016/j.scienta.2020.109567

Cited by 34 publications

(30 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…13,19,20 In addition to regulating anthocyanin synthesis, MiMYB1 also contributes to the transport of anthocyanins in mango. 21 In Litchi, LcMYB1 interacts with LcbHLH3 to activate LcDFR expression. 12 The interaction of PybHLH3 and PyWRKY26 can synergistically activate the PyMYB114 promoter, leading to anthocyanin accumulation in red-skinned pears.…”

Section: ■ Introductionmentioning

confidence: 99%

“…In tomato, SIMYB75 has been identified as a key factor that determines fruit quality by promoting the accumulation of anthocyanins and the production of volatile aromas . In Arabidopsis, the transcription factors MYB11, MYB12, and MYB111 activate CHS, CHI, and F3H, early genes in the anthocyanin biosynthesis pathway, and the transcription factors PAP1, PAP2, MYB113, MYB114, and TT2 combine with TT8 to activate later genes in the pathway. ,, In addition to regulating anthocyanin synthesis, MiMYB1 also contributes to the transport of anthocyanins in mango . In Litchi, LcMYB1 interacts with LcbHLH3 to activate LcDFR expression .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolomic and Transcriptomic Analyses of Anthocyanin Biosynthesis Mechanisms in the Color Mutant Ziziphus jujuba cv. Tailihong

Shi

Zhu

et al. 2020

J. Agric. Food Chem.

View full text Add to dashboard Cite

The purplish-red color of “Tailihong” jujube fruit skins is caused primarily by anthocyanin accumulation, but the mechanisms that underlie anthocyanin biosynthesis in jujube fruit have rarely been studied. We performed metabolomic and transcriptomic analyses of jujube fruit skins at different developmental stages and identified a total of 158 flavonoids, among which cyanidin-3-O-rutinoside and peonidin-3,5-O-diglucoside were the primary anthocyanins. During fruit development and maturation, the anthocyanin content was strongly correlated with the expression of ZjANS and ZjUGT79B1, suggesting that these are key genes in the anthocyanin biosynthesis process. Transcriptomic analysis indicated that the transcription factors ZjMYB5, ZjTT8, and ZjWDR3 regulated anthocyanin biosynthesis in jujube fruit skins. Subcellular localization experiments confirmed that ZjANS and ZjUGT79B1 were localized to the nucleus and the endoplasmic reticulum. ZjMYB5 and ZjTT8 were found only in the nucleus, whereas strong fluorescence signals from ZjWDR3 were observed in the nucleus and cytoplasm. Prokaryotic expression and in vitro enzyme activity assays showed that the recombinant ZjANS protein catalyzed the formation of cyanidin from (+)-catechin. Secondary glycosylation by ZjUFGT79B1 modified cyanidin-3-O-glucoside to produce cyanidin-3-O-rutinoside, and ZjCCoAOMT readily catalyzed the production of the methylated anthocyanin peonidin-3,5-O-diglucoside from cyanidin 3,5-O-glucoside. Dual-Luciferase and GUS activity assays showed that the ZjANS and ZjUGT79B1 promoters were activated by ZjMYB5, ZjTT8, and ZjWDR3. All data indicated that these three transcription factors played important roles in anthocyanin biosynthesis in the color mutant Ziziphus jujuba cv. Tailihong, contributing to anthocyanin accumulation by enhancing the expression of ZjANS and ZjUGT79B1.

show abstract

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metabolomic and Transcriptomic Analyses of Anthocyanin Biosynthesis Mechanisms in the Color Mutant Ziziphus jujuba cv. Tailihong

Shi

Zhu

et al. 2020

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…In tomato ( Solanum lycopersicum ), SlMYB14 was involved in the regulation of flavonoid biosynthesis, and played a role in maintaining plant active oxygen homeostasis ( Li et al., 2021 ). In mango ( Mangifera indica L.), MiMYB1 acts as a critical regulator of anthocyanin biosynthesis and regulates the light-dependent red coloration ( Kanzaki et al., 2020 ). PpMYB17 , a bHLH or WD40 cofactor in the MBW complex, activates the structural genes PpCHS , PpCHI , PpF3H , and PpFLS involved in flavonoid biosynthetic pathway, and was positively regulates flavonoid biosynthesis in pear ( Pyrus spp.)…”

Section: Introductionmentioning

confidence: 99%

Integrated analysis of multi-omics and fine-mapping reveals a candidate gene regulating pericarp color and flavonoids accumulation in wax gourd (Benincasa hispida)

et al. 2022

View full text Add to dashboard Cite

Wax gourd (Benincasa hispida), a popular fruit of the Cucurbitaceae (cucurbits) family, contains many nutrients with health benefits and is widely grown in China and other tropical areas. In this study, a wax gourd mutant hfc12 with light-color pericarp was obtained through ethane methylsulfonate (EMS) mutagenesis. Integrative analysis of the metabolome and transcriptome identified 31 differentially accumulated flavonoids (DAFs; flavonoids or flavonoid glycosides) and 828 differentially expressed genes (DEGs) between the hfc12 mutant and wild-type ‘BWT’. Furthermore, BSA-seq and kompetitive allele specific PCR (KASP) analysis suggested that the light-color pericarp and higher flavonoid content was controlled by a single gene BhiPRR6 (Bhi12M000742), a typical two-component system (TCS) pseudo-response regulator (PRR). Genetic analysis detected only one nonsynonymous mutation (C-T) in the second exon region of the BhiPRR6. Weighted correlation network analysis (WGCNA) identified the downstream target genes of BhiPRR6, probably regulated by light and were intermediated in the regulatory enzyme reaction of flavonoid biosynthetic pathway. Thus, these results speculated that the transcription factor BhiPRR6, interacting with multiple genes, regulates the absorption of light signals and thereby changes the pericarp color and synthesis of flavonoids in wax gourd.

show abstract

“…The allelic variations of the transcription factors were associated with the phenotype variation relative to anthocyanin biosynthesis. A lot of R2R3-MYB TFs had been identi ed as the key regulators of anthocyanin accumulation and the tissue coloration in some plants, such as MiMYB1 in mango [21], MdMYBA and MdMYB10 in apple [22,23],VvMYB114, VvMYB5b and VvMYBAs in grape [24][25][26], PcMYB114 in pear [27], PaMYB10 in sweet cherry [28], VcMYBA in blueberry [29] and PpMYB10.1 and PpMYB10.3 in peach [30].…”

Section: Introductionmentioning

confidence: 99%

Functional R2R3-MYB Transcription Factor NsMYB1, Regulating Anthocyanin Biosynthesis, Was Relative To The Fruit Color Differentiation in Nitraria Sibirica Pall

Xuemei

Zong

et al. 2021

Preprint

View full text Add to dashboard Cite

Background Nitraria sibirica Pall. is an economic plant with two kinds of fruit color, widely spreads in the Qinghai Tibet Plateau. The chemical analysis and pharmacological evaluation had been carried out for several tens of years, the mechanism behind the fruit color differentiation is still unclear. Results In this manuscript, the chemical analysis of the extractions showed that the chemical composition of fruit color was anthocyanin, and two kind of Nitraria sibirica Pall. were caused by the content differentiation with the same anthocyanin kinds. Cya-nidin-3-[2ʹ’-(6ʹ’’-coumaroyl)-glucosyl]-glucoside (C3G) was the major anthocyanin. Transcriptome analysis and the qRT-PCR revealed that the structural genes relative to anthocyanin biosynthesis except CHS, F3’5’H and ANS were up-regulated in BF compared with RF, which indicated that transcript factor should be the reason for the expression difference of the structure genes. In the unigenes of the transcript factor MYB and bHLH, relative to anthocyanin, only NsMYB1 (Clus-ter-8422.10600), was high-expression and up-expression in the BF. NsMYB1 encoded the same length protein with four amino acid differences in the RF and BF, and both contained the intact DNA, HTH-MYB and SANT domains. NsMYB1 was close to the AtMYB114, AtMYB113 and AtPAP1, regulating anthocyanin biosynthesis, in phylogenetic relationship. Both NsMYB1r and NsMYB1b could promote the transcript of the structural genes, and induced the anthocyanin accumulation in all tissues of transgenic tobacco. The insertion of ‘TATA’ in the promoter of NsMYB1r gave one more promoter region, and was the reason for higher transcripts in black fruit possibly. Conclusions NsMYB1 was a functional R2R3-MYB transcription factor, regulated the anthocyanin biosynthesis, and leaded to the fruit color differentiation in Nitraria sibirica Pall.

show abstract

The R2R3-MYB transcription factor MiMYB1 regulates light dependent red coloration of ‘Irwin’ mango fruit skin

Cited by 34 publications

References 70 publications

Metabolomic and Transcriptomic Analyses of Anthocyanin Biosynthesis Mechanisms in the Color Mutant Ziziphus jujuba cv. Tailihong

Metabolomic and Transcriptomic Analyses of Anthocyanin Biosynthesis Mechanisms in the Color Mutant Ziziphus jujuba cv. Tailihong

Integrated analysis of multi-omics and fine-mapping reveals a candidate gene regulating pericarp color and flavonoids accumulation in wax gourd (Benincasa hispida)

Functional R2R3-MYB Transcription Factor NsMYB1, Regulating Anthocyanin Biosynthesis, Was Relative To The Fruit Color Differentiation in Nitraria Sibirica Pall

Contact Info

Product

Resources

About