The Rab GTPase Ypt7 is linked to retromer-mediated receptor recycling and fusion at the yeast late endosome

Balderhaar, Henning J. kleine; Arlt, Henning; Ostrowicz, Clemens W.; Bröcker, Cornelia; Sündermann, Frederik; Brandt, Roland; Babst, Markus; Ungermann, Christian

doi:10.1242/jcs.071977

Cited by 102 publications

(118 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is now known that the yeast Mon1-Ccz1 complex is the GEF of Rab7-like Ypt7 protein , which was confirmed recently with the mammalian complex (Gerondopoulos et al, 2012). On late endosomes, activated Rab7-GTP can bind the membrane-remodeling retromer complex and might thus support the recycling of receptors from late endosomes (Balderhaar et al, 2010;Liu et al, 2012;Rojas et al, 2008;Seaman et al, 2009). In addition, and probably later on, Rab7 also promotes fusion with the lysosome, although the exact order has not been clarified (Balderhaar et al, 2010;Huotari and Helenius, 2011;Liu et al, 2012).…”

Section: Introductionmentioning

confidence: 82%

CORVET and HOPS tethering complexes–coordinators of endosome and lysosome fusion

Balderhaar

Ungermann

2013

Journal of Cell Science

Self Cite

470

View full text Add to dashboard Cite

SummaryProtein and lipid transport along the endolysosomal system of eukaryotic cells depends on multiple fusion and fission events. Over the past few years, the molecular constituents of both fission and fusion machineries have been identified. Here, we focus on the mechanism of membrane fusion at endosomes, vacuoles and lysosomes, and in particular on the role of the two homologous tethering complexes called CORVET and HOPS. Both complexes are heterohexamers; they share four subunits, interact with Rab GTPases and soluble NSF attachment protein receptors (SNAREs) and can tether membranes. Owing to the presence of specific subunits, CORVET is a Rab5 effector complex, whereas HOPS can bind efficiently to late endosomes and lysosomes through Rab7. Based on the recently described overall structure of the HOPS complex and a number of in vivo and in vitro analyses, important insights into their function have been obtained. Here, we discuss the general function of both complexes in yeast and in metazoan cells in the context of endosomal biogenesis and maturation.

show abstract

Section: Introductionmentioning

confidence: 82%

CORVET and HOPS tethering complexes–coordinators of endosome and lysosome fusion

Balderhaar

Ungermann

2013

Journal of Cell Science

Self Cite

470

View full text Add to dashboard Cite

show abstract

“…Retromer recruitment and tubule biogenesis may be coordinated by RAB5-to-RAB7 conversion (Rojas et al 2008;Seaman et al 2009;Balderhaar et al 2010;Liu et al 2012;van Weering et al 2012), and recent lines of evidence indicate that this process may also involve the actin cytoskeleton (Seaman 2012). During ILV formation, the sorting of ubiquitinated cargo proteins is achieved by ESCRT complexes, which may also be involved in the membrane deformation and scission processes (Wollert and Hurley 2010;Henne et al 2012).…”

Section: Lipid-mediated Endosomal Dynamicsmentioning

confidence: 99%

Lipid Sorting and Multivesicular Endosome Biogenesis

Bissig

Grüenberg

2013

Cold Spring Harbor Perspectives in Biology

133

107

View full text Add to dashboard Cite

Intracellular organelles, including endosomes, show differences not only in protein but also in lipid composition. It is becoming clear from the work of many laboratories that the mechanisms necessary to achieve such lipid segregation can operate at very different levels, including the membrane biophysical properties, the interactions with other lipids and proteins, and the turnover rates or distribution of metabolic enzymes. In turn, lipids can directly influence the organelle membrane properties by changing biophysical parameters and by recruiting partner effector proteins involved in protein sorting and membrane dynamics. In this review, we will discuss how lipids are sorted in endosomal membranes and how they impact on endosome functions.

show abstract

“…Two studies support this idea. First, overexpressed yeast Rab7 (Ypt7) causes a premature fusion with vacuoles and delivery of cargo receptors to the vacuole surface (Balderhaar et al , 2010 ). Second, mutants in retromer that bind efficiently to Rab7 interfere with vacuole morphology, likely due to a prolonged interaction of retromer with Rab7 (Liu et al , 2012 ).…”

Section: Even Though the Exact Organization Of Such Zonesmentioning

confidence: 99%

Cellular microcompartments constitute general suborganellar functional units in cells

Holthuis

Ungermann

2013

Biological Chemistry

Self Cite

View full text Add to dashboard Cite

All cells are compartmentalized to facilitate enzymatic reactions or cellular dynamics. In eukaryotic cells, organelles differ in their protein/lipid repertoire, luminal ion composition, pH, and redox status. In addition, organelles contain specialized subcompartments even within the same membrane or within its lumen. Moreover, the bacterial plasma membrane reveals a remarkable degree of organization, which is recapitulated in eukaryotic cells and often linked to cell signaling. Finally, protein-based compartments are also known in the bacterial and eukaryotic cytosol. As the organizing principle of such cellular subcompartments is likely similar, previous definitions like rafts, microdomains, and all kinds of ' -somes ' fall short as a general denominator to describe such suborganellar structures. Within this review, we will introduce the term cellular microcompartment as a general suborganellar functional unit and discuss its relevance to understand subcellular organization and function.

show abstract

The Rab GTPase Ypt7 is linked to retromer-mediated receptor recycling and fusion at the yeast late endosome

Cited by 102 publications

References 43 publications

CORVET and HOPS tethering complexes–coordinators of endosome and lysosome fusion

CORVET and HOPS tethering complexes–coordinators of endosome and lysosome fusion

Lipid Sorting and Multivesicular Endosome Biogenesis

Cellular microcompartments constitute general suborganellar functional units in cells

Contact Info

Product

Resources

About