The Radiation Chemistry of Cytochrome c

Abstract

The literature on the reaction of cytochrome c with the radiolytically generated radicals \documentclass{article}\pagestyle{empty}\begin{document}$ {\rm e}_{{\rm eq}}^ -,^. {\rm OH,}^{\rm .} {\rm H,CO}_2^ -,{\rm O}_{\rm 2}^ -,{\rm Br}_{\rm 2}^ - $\end{document} and various organic radicals is reviewed. It would appear that negatively charged radicals, aided by the electric field of cytochrome c, react at the exposed haem edge. Uncharged organic radicals also react at this site. \documentclass{article}\pagestyle{empty}\begin{document}$ ^. {\rm H} $\end{document} and \documentclass{article}\pagestyle{empty}\begin{document}$ ^. {\rm OH} $\end{document} are likely to reduce the prosthetic group indirectly by a tunnelling mechanism.

Synthesis, X-ray crystal structure, antimicrobial activity and photodynamic effects of some thiabendazole complexes

“…The light bluish powder was filtered off, washed with cold methanol and dried in vacuo over silica gel. Elemental analyses were consistent with the stoichiometry C20 H 16 CuN 8 S 2 O 7 (yield 74.5%). Found C, 39.44; H, 2.67; N, 18.49; Cu, 10.39; S, 10.65%.…”

“…For measurement of O ÀÅ 2 on photogeneration, the metal complexes were photolysed in the presence of ferricytochrome c (50 lM) in 50 mM phosphate buffer (pH ¼ 7.4) and the reduction was monitored spectrophotometrically at 550 nm, where e max for ferricytochrome c is 0.99 Â 10 4 M À1 cm À1 and e max for ferrocytochrome c is 2.99 Â 10 4 M À1 cm À1 . Hence De 550 ¼ 20; 000 M À1 cm À1 was used for reduced -oxidised cytochrome c [20][21][22]. To study enzymatic production of superoxide, similar experiments were carried out with cytochrome c reductase in the dark.…”

β-Carbolines That Accumulate in Human Tissues May Serve a Protective Role against Oxidative Stress

“…, and the samples containing 20 M ferricytochrome c in 20 mM phosphate buffer, pH 7.4, were illuminated at 330 nm in the absence and presence of test compounds, in the instrument and under the conditions described above. The reduction of ferricytochrome c was monitored spectrophotometrically at 550 nm, using ⑀ 500 ϭ 20,000 M Ϫ1 cm Ϫ1 for the reduced-oxidized cytochrome c (28).…”

Protein‐associated pigments that accumulate in the brunescent eye lens

“…The superoxide anion (O;-) was detected using the cytochrome c reduction method [24]. Solutions of photosensitizers were illuminated in the presence of ferricytochrome c in 50 mM phosphate buffer, pH 7.4, and the reduction monitored spectrophotometrically at 550 nm, using sSOO = 20,000 M-' 'cm-' for the reduced-oxidized cytochrome c [25]. A concentration of 20 PM was used throughout.…”