The radiation resistance of long-lived lymphocytes and plasma cells in mouse and rat lymph nodes

Studies of Non-Migrating, Long-Lived Lymphocytes in Rats and Mice

“…The failure of the anti-lymphocyte sera to change significantly the proportion of long-lived lymphocytes in lymph nodes is different from what might have been expected from 2 previously reported lines of work: (1) the resistance of long-lived sessile lymphocytes to immunolytic agents [9,10], and (2) the specific sensitivity of circulating, long-lived lymphocytes to anti-lymphocyte sera [16]. The absence of a significant change in proportion of the long-lived cells in the current experiments means that they are neither specifically sensitive nor specifically resist ant to the anti-lymphocyte sera.…”

“…Three batches of heterologous anti-lymphocyte sera were prepared, all by the method of Levey and Medawar [9]. These were: (1) anti-mouse lymph node cell serum prepared against a mixture of cells from C3H and CBA mice, (2) anti-rat lymph node cell serum and (3) an ti-rat thymus cell serum.…”

“…The sessile, long-lived lymphocytes react to antigens in exactly this way [13]. On the other hand, if they are only a randomly trapped population of the circulating pool of thymus-derived cells, it is not easy to explain their resistance to immunolytic agents other than anti-lymphocyte serum [9,10]. Furthermore, preliminary data suggest that these cells are rela tively increased in neonatally thymectomized rats.…”

Replication of scrapie in spleens of SCID mice follows reconstitution with wild-type mouse bone marrow

“…The presence of cells with the morphology and location of FDCs in the spleen and lymph nodes of mice, which immunostain with anti-PrP and entrap immune complexes supports this suggestion (McBride et aI., 1992). However, a radiation-resistant population of lymphocytes is recognized (Miller & Cole, 1967). A new opportunity to resolve this uncertainty and to further define the role of the immune system in the peripheral pathogenesis of scrapie has arisen from the availability of the severely combined immunodeficient (SCID) mouse, which shows a resistance to peripheral infection with a murine strain of Creutzfeldt-Jakob disease (Kitamoto et al, 1991).…”

The Effect of Phytohemagglutinin in Rabbits

“…It has been demonstrated that lymphocytes [22][23][24][25][26] and plasma cells [22] can survive for long periods of time in the autologous host without undergoing morpho logical alterations. Whether 'free' cells can survive in homologous hosts without being rejected remains to be proven.…”