The radioimmunoassay of aldosterone in serum and urine: Theoretical and practical aspects

Ekins, Roger; Newman, G. B.; Piyasena, R. D.; Banks, P.; Slater, J. D. H.

doi:10.1016/0022-4731(72)90076-3

Cited by 58 publications

(24 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The current mainstay for measuring aldosterone is by antibody-based methods. These methods can be direct or require initial extraction of plasma or serum using liquid-liquid extraction, solid-phase extraction, or chromatographic methods (2)(3)(4)(5)(6). Overall, immunoassays have problems with varying selectivity and poor interlaboratory reproducibility, requiring each laboratory to establish its own reference range for the diagnosis of PAL (7 ).…”

mentioning

confidence: 99%

Measurement of Aldosterone in Human Plasma by Semiautomated HPLC–Tandem Mass Spectrometry

et al. 2009

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

Measurement of Aldosterone in Human Plasma by Semiautomated HPLC–Tandem Mass Spectrometry

et al. 2009

View full text Add to dashboard Cite

show abstract

“…The use of precision profiles in assays dates back to Ekins et al (1972) and Ekins (1978Ekins ( , 1983. On occasion, they have also been referred to as imprecision profiles (e.g.…”

Section: Introductionmentioning

confidence: 99%

A Bayesian precision profile for measuring the quality of immunoassay experiments

O’Malley¹

2008

Phil. Trans. R. Soc. A.

View full text Add to dashboard Cite

A precision profile, the relationship between the concentration of a substance and its measured precision, is a convenient way of conveying the ability of an immunoassay to accurately measure the concentration of a substance in blood serum. A precision profile is characterized by the definition of precision. Historically, precision has been evaluated as the standard error of an estimator of the concentration in a sample conditional on the true concentration. In this paper, Bayesian predictive inference is used to develop a new measure of precision based on the accuracy with which an assay could infer the concentration in a hypothetical new sample. This leads to a natural procedure for evaluating a precision profile that avoids using approximations such as those inherent in traditional methods.

show abstract

“…Some of these procedures include paper (2,7,10,15,25) and thin-layer chromatography (22) or chromatography on Sephadex LH-20 (3,5,10,29). Some direct methods without any purification of the extract have recently been reported (12, 14.…”

Section: H Adlercreutz Dept Of Clinical Chemistry University Of Hmentioning

confidence: 99%

Evaluation of Radioimmunological Methods for Assay of Plasma and Urinary Aldosterone

Pakarinen

Koskinen

Adlercreutz

1976

Scandinavian Journal of Clinical and Laboratory Investigation

View full text Add to dashboard Cite

Two radioimmunological methods for assay of plasma and urinary aldosterone were carefully evaluated. In the plasma method a radioimmunoassay is preceded by chromatography on a Sephadex LH-20 column. The method for urine includes a preextraction, hydrolysis of the acid-labile conjugates of aldosterone, and a radioimmunoassay. Both methods fulfill the criteria of reliability and are suitable for both routine and demanding research assays. The plasma method, using columns of double length, is also applicable to analysis of aldosterone in plasma of newborn children, and pregnant females and in cord plasma. The concentration of plasma aldosterone in healthy subjects on an ad lib salt diet was 162 +/- 93 (S.D.) pmol/1 in the supine position and 312 +/- 217 (S.D.) pmol/1 upright. The urinary excretion of aldosterone in healthy subjects was 28.3 +/- 16.7 (S.D.) nmol/24 h.

show abstract

The radioimmunoassay of aldosterone in serum and urine: Theoretical and practical aspects

Cited by 58 publications

References 5 publications

Measurement of Aldosterone in Human Plasma by Semiautomated HPLC–Tandem Mass Spectrometry

Measurement of Aldosterone in Human Plasma by Semiautomated HPLC–Tandem Mass Spectrometry

A Bayesian precision profile for measuring the quality of immunoassay experiments

Evaluation of Radioimmunological Methods for Assay of Plasma and Urinary Aldosterone

Contact Info

Product

Resources

About