The random amplification of polymorphic DNA for fingerprinting plants.

Munthali, M; Ford‐Lloyd, B. V.; Newbury, H. J.

doi:10.1101/gr.1.4.274

Cited by 67 publications

(36 citation statements)

References 10 publications

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“…19) The optimized conditions were as follows: 200 ng of DNA, 200 mm dNTP, 1.5 mm MgCl 2 , 2.5 U of Taq polymerase, 35cycles, 55°C of annealing temperature (data not shown).…”

Section: Initial Screening Of Polymorphic Rapd Primers On Four Ginsenmentioning

confidence: 99%

Molecular Authentication of Panax ginseng Species by RAPD Analysis and PCR-RFLP.

Chung

Kim

et al. 2001

Biological & Pharmaceutical Bulletin

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In order to develop convenient and reproducible methods for the identification of ginseng drugs at a DNA level, randomly amplified polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) analyses were applied within Panax species. To authenticate Panax ginseng among ginseng populations, RAPD analysis was carried out using a 20 mer-random primer. The similarity coefficients among the DNA of ginseng plants analyzed were low, ranging from 0.197 to 0.491. In addition, by using PCR-RFLP analysis, very different fingerprints were obtained within Korean ginseng plants. These results suggest that these methods are able to authenticate the concerned Panax species. Broader application of this approach to authenticate other morphologically similar medicinal materials is rationalized.

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“…19) The optimized conditions were as follows: 200 ng of DNA, 200 mm dNTP, 1.5 mm MgCl 2 , 2.5 U of Taq polymerase, 35cycles, 55°C of annealing temperature (data not shown).…”

Section: Initial Screening Of Polymorphic Rapd Primers On Four Ginsenmentioning

confidence: 99%

Molecular Authentication of Panax ginseng Species by RAPD Analysis and PCR-RFLP.

Chung

Kim

et al. 2001

Biological & Pharmaceutical Bulletin

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“…In recent years a number of molecular markers such as random amplified polymorphic DNA (RAPD) (Hu and Quiros, 1991;Munthali et al, 1992), amplified fragment length polymorphism (AFLP) (Vos et al, 1995), simple sequence repeats (SSR) (Zietkiewicz et al, 1994) and intersimple sequence repeats (ISSR) (Salimath et al, 1995;Wolfe and Randle, 2001) have been widely used to detect genetic diversity in plants (Karp et al, 1996;Wolfe and Liston, 1998;Nan et al, 2003;Tang et al, 2003). In particular, ISSR markers can be highly variable within a species and have the advantage over RAPD markers that they use longer primers that allow more stringent annealing temperatures and reveal many more polymorphic fragments.…”

Section: Introductionmentioning

confidence: 99%

Determination of genetic variation in Rhodiola crenulata from the Hengduan Mountains Region, China using inter-simple sequence repeats

et al. 2006

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The plant Rhodiola crenulata is a perennial herbaceous species distributed in the plateau region of southwestern China, especially the Hengduan Mountains region. It has been one of the most important traditional herbal remedies in Tibet for more than one thousand years, but the accelerated and uncontrolled collection of this plant since the 1980s has lead to deforestation. We used inter-simple sequence repeats (ISSR) to assess levels of genetic variation in R. crenulata from nine diverse natural populations in eastern Tibet and northern Yunnan, the first time such a study has been carried out. The 12 primers we used were able to detect 184 polymorphic loc. Analysis of molecular variance (AMOVA) indicated that species level genetic diversity was relatively high (p = 97.83%, and H o = 0.464) and analysis using Shannon's index showed that the within and between genetic diversity of R. crenulata are approximately equal. Nei's genetic distance and unweighted pair-group method with arithmetic averages (UPGMA) cluster analysis showed that the three populations from Tibet and the six populations from Yunnan form two major clusters. The Yunnan populations from three locations were further divided into three corresponding groups, indicating that genetic differentiation was correlated to geographic distribution. Understanding the genetic structure of R. crenulata provides insight for the conservation and management of this endangered species.

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“…Target DNA was randomly amplified by PCR using these arbitrary primers (DNA amplification fingerprinting No prior knowledge of the DNA sequence for the targeted gene is required, as the primers will bind somewhere in the sequence, but it is not certain exactly where. There are some disadvantages with these markers-detection of polymorphism is limited, dominant markers (difficult to distinguish between homo and heterozygotes); very sensitive to PCR conditions (Munthali et al, 1992;Lowe et al, 1996) and this may lead to poor reproducibility.…”

Section: Randomly Amplified Polymorphic Dna (Rapd)mentioning

confidence: 99%

Molecular Markers- Characteristics and Applications in Animal Breeding

Athe¹,

Naha²,

Neerasa³

et al. 2018

Int. J. Livest. Res.

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The random amplification of polymorphic DNA for fingerprinting plants.

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Cited by 67 publications

References 10 publications

Molecular Authentication of Panax ginseng Species by RAPD Analysis and PCR-RFLP.

Molecular Authentication of Panax ginseng Species by RAPD Analysis and PCR-RFLP.

Determination of genetic variation in Rhodiola crenulata from the Hengduan Mountains Region, China using inter-simple sequence repeats

Molecular Markers- Characteristics and Applications in Animal Breeding

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