The rapid discovery and identification of physalins in the calyx of Physalis alkekengi L.var.franchetii (Mast.) Makino using ultra-high performance liquid chromatography–quadrupole time of flight tandem mass spectrometry together with a novel three-step data mining strategy

Huang, Cai Ping; Xu, Qiong; Chen, Chang; Song, Chengwu; Xu, Yong; Xiang, Yang; Feng, Yulin; Ouyang, Hui; Zhang, Yang; Jiang, Hongliang

doi:10.1016/j.chroma.2014.08.004

Cited by 42 publications

(33 citation statements)

References 16 publications

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“…Among them, physalin A, physalin B, physalin D, physalin D′, physalin F, physalin L, physalin N, physalin O, 3 α ‐hydroxy‐2,3‐dihydro‐4,7‐didehydroneophysalin B, 4,7‐didehydroneophysalin B and 7 β ‐hydroxyphysalin A were identified by comparison with reference standards. All of the detected physalins shared similar fragmentation pathways which have been well documented (Huang et al, ). The basic skeleton of physalins could be designated as two portions: moieties A and B.…”

Section: Resultssupporting

confidence: 72%

Identification of absorbed constituents and in vivo metabolites in rats after oral administration of Physalis alkekengi var. franchetii by ultra‐high‐pressure liquid chromatography quadrupole time‐of‐flight mass spectrometry

Feng

Huo

Liu

et al. 2017

Biomedical Chromatography

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The calyces of Physalis alkekengi var. franchetii (Chinese Lantern, JDL) are well-known as traditional Chinese medicine owing to its various therapeutic effects. However, the bioactive constituents responsible for the pharmacological effects of JDL and their metabolites in vivo are still unclear to date. In this paper, an ultra-high-pressure liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS/MS) method was established to identify absorbed constituents and in vivo metabolites in rat biological fluids after oral administration of JDL. Based on the proposed strategy, 33 compounds were observed in dosed rat biosamples. Twelve of 33 compounds were indicated as prototype components of JDL, and 21 compounds were predicted to be metabolites of JDL. Finally, the metabolic pathways were proposed, which were glucuronidation, sulfation, methylation and dehydroxylation for flavonoid constituents and sulfonation and hydroxylation for physalin consitituents. This is the first systematic study on the absorbed constituents and metabolic profiling of JDL and will provide a useful template for screening and characterizing the ingredients and metabolites of traditional Chinese medicine.

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Section: Resultssupporting

confidence: 72%

Identification of absorbed constituents and in vivo metabolites in rats after oral administration of Physalis alkekengi var. franchetii by ultra‐high‐pressure liquid chromatography quadrupole time‐of‐flight mass spectrometry

Feng

Huo

Liu

et al. 2017

Biomedical Chromatography

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“…To this effect, it is essential to know the chemical composition of Physalis peruviana calyx residues, as well as the properties of these compounds. In this sense, flavonoids, terpenoids and glycosides have been identified in an extract obtained from calyces of Physalis peruviana and in addition, a withanolide (28‐hydroxywithanolide E) was isolate from the calyces of Physalis peruviana and physalins have also been identified in the calyx of the genus Physalis . Currently, there are some reports that inform about the anti‐inflammatory activity of Physalis peruviana calyces by using in vitro and in vivo models and this plant organ may be a promising source of new therapeutic agents for the treatment of specific diseases.…”

Section: Introductionmentioning

confidence: 99%

Potential of Physalis peruviana calyces as a low‐cost valuable resource of phytoprostanes and phenolic compounds

et al. 2018

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BACKGROUND: In Colombia, agro-industrial residues represent an enormous economic and environmental problem, which could be reduced if different techniques for the addition of value to such residues were implemented by this industrial sector. One of the fruits with the highest export rates is Physalis peruviana (goldenberry); however, this fruit is generally marketed without its calyx, generating a large amount of residues. To develop a strategy to add value to these residues, it is essential to know their chemical composition. RESULTS: In the present work, phytoprostanes (PhytoPs) -new active oxylipins -have been detected for the first timein Physalis peruviana calyces by ultra-high performance liquid chromatography triple quadrupole tandem mass spectrometry (UHPLC-QqQ-MS/MS), F 1t -phytoprostanes and D 1t -phytoprostanes being the predominant and minor classes, respectively. In addition, we were able to characterize the phenolic compounds profile of this matrix using LC-IT-DAD-MS/MS, describing six phenolic derivatives for the first time therein. CONCLUSIONS: This study increases our knowledge of the chemical composition of the calyces of this fruit and thereby supports the recycling of this class of residue. Consequently, goldenberry calyces could be used as phytotherapeutic, nutraceutic, or cosmetic ingredients for the development of diverse natural products. ABBREVIATIONS Bis-TrisBis-(2-hydroxyethyl)-amino-tris(hydroxymethyl) -methane BHA tert-butylhydroxyanisole LC-MS liquid chromatography-mass spectrometry EXPERIMENTAL Chemicals and materials Ten PhytoP standards ( Fig. 1: 9-F 1t -PhytoP, 9-epi-9-F 1t -PhytoP, ent-16-F 1t -PhytoP, ent-16-epi-16-F 1t -PhytoP, 9-D 1t -PhytoP, Figure 1. Chemical structures of the PhytoPs analyzed in Physalis peruviana calyces.

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“…The compounds with the same or similar parent nuclei can be cleaved from the same characteristic fragments under the same MS conditions. According to this characteristic fragment, different structures in TCM can be classified and identified . NL means that the compounds of the same or similar structure lose the same neutral fragment under the same mass conditions.…”

Section: Introductionmentioning

confidence: 99%

“…According to this characteristic fragment, different structures in TCM can be classified and identified. 13 NL means that the compounds of the same or similar structure lose the same neutral fragment under the same mass conditions. NLs are essential for screening substances.…”

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confidence: 99%

Establishing a rapid classification and identification method for the major triterpenoids of Alisma orientale

Yang

Dong

et al. 2019

Phytochemical Analysis

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Introduction Alismatis Rhizoma (AR) has been widely used to treat various diseases. Its complex chemical composition has caused certain difficulties in the analysis of this traditional Chinese medicine. Therefore, it is necessary to establish a method for the rapid classification and identification of the chemical constituents of AR. Objective This article describes a method for the rapid classification and identification of major triterpenoids in AR. Methodology The samples were analysed by ultra‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry (UPLC‐Q‐TOF‐MS). The assay was performed on a Waters ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with 0.1% formic acid in water (A), and acetonitrile (B) as mobile phase by gradient elution at a flow rate of 0.3 mL/min. In the positive ion mode, the fragment information was obtained and compared with the characteristic fragments and neutral losses described in the literature. Then, the rapid classification and identification of the chemical components from AR were achieved. Results Finally, 25 triterpene compounds of AR were identified. Conclusions The method established in this study achieved the rapid classification and identification of chemical components in AR, which promotes the development of research methods to study the constituents of traditional Chinese medicine.

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The rapid discovery and identification of physalins in the calyx of Physalis alkekengi L.var.franchetii (Mast.) Makino using ultra-high performance liquid chromatography–quadrupole time of flight tandem mass spectrometry together with a novel three-step data mining strategy

Cited by 42 publications

References 16 publications

Identification of absorbed constituents and in vivo metabolites in rats after oral administration of Physalis alkekengi var. franchetii by ultra‐high‐pressure liquid chromatography quadrupole time‐of‐flight mass spectrometry

Identification of absorbed constituents and in vivo metabolites in rats after oral administration of Physalis alkekengi var. franchetii by ultra‐high‐pressure liquid chromatography quadrupole time‐of‐flight mass spectrometry

Potential of Physalis peruviana calyces as a low‐cost valuable resource of phytoprostanes and phenolic compounds

Establishing a rapid classification and identification method for the major triterpenoids of Alisma orientale

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