The Rapid Identification of Enteric Organisms by Microtechnique

Bicknell, Alice K.; Butt, Fahad Fayyaz; Mattman, Lida H.

doi:10.2105/ajph.42.4.437

Cited by 6 publications

(4 citation statements)

References 0 publications

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“…In 1952 Bicknell et al (1) reported on a semisolid medium which allowed screening for Sahnonella and Shigella spp. to be carried out in three and a half hours.…”

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confidence: 99%

Presumptive identification ofSalmonella enterica using two rapid tests

Ruiz

Varela

Sempere

et al. 1991

Eur. J. Clin. Microbiol. Infect. Dis.

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Two rapid test for early detection of Salmonella enterica, the cytochrome oxidase test and a fluorescence test, were evaluated in 1,200 colonies which had been isolated from human feces and cultured on MacConkey and Salmonella-Shigella media. Using the fluorescence test there were no false negative results (sensitivity 100%) and of 205 positive cases 62 did not correspond biochemically to salmonella (specificity 94.1%); 44 of these 62 were positive in the cytochrome oxidase test, raising the specificity to 98.2%. In conclusion, the combination of the two tests could be very useful due to the ease of performance, low cost and excellent results obtained.

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“…In 1952 Bicknell et al (1) reported on a semisolid medium which allowed screening for Sahnonella and Shigella spp. to be carried out in three and a half hours.…”

mentioning

confidence: 99%

Presumptive identification ofSalmonella enterica using two rapid tests

Ruiz

Varela

Sempere

et al. 1991

Eur. J. Clin. Microbiol. Infect. Dis.

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“…Various methods have been described for the rapid detection or screening of enteric pathogens, or both (1,12). To our knowledge the API Z system is the only one to be commercially available.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a rapid method to exclude the presence of certain enteric pathogens in stool specimens

Teti¹,

Burdash²,

Zamboni³

et al. 1984

J Clin Microbiol

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A new commercial method intended to exclude the presence of Salmonella spp., Shigella spp., and Yersinia enterocolitica and to presumptively identify Salmonella isolates within 2 h after primary isolation from stool specimens was evaluated. This system is marketed in Europe as API Z and in the United States as Rapid SST. The strip consists of five pairs of cupules for the screening of five lactose-negative colonies. The first cupule of each pair detects the presence of five enzymatic activities, whereas the second serves to maintain the strain for additional testing if necessary. A total of 197 fresh isolates from stool specimens and 217 stock cultures of Salmonella spp., Shigella spp., and Yersinia enterocolitica were tested, with the API 20E system as a reference method. In the stool specimens, 77.3% of the bacteria could be excluded from further workup for the presence of these organisms within 2 h. Over 97% of the stock strains and each of three fresh Salmonella isolates tested produced a reaction pattern corresponding to a correct presumptive identification. This reaction pattern was not produced by any isolate other than the Salmonella isolates. The API Z system can be used as a screen for the presence of Salmonella and Shigella spp. and can provide an accurate presumptive identification of Salmonella isolates within 2 h after primary isolation.

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“…Acetoin (acetylmethylcarbinol) test made directly 5. Transferring of 0.5 ml to a tube of semisolid Simmons citrate medium and incubation in a water bath at 35°C; the tube is 45 X 5 mm and contains approximately 1.5 ml of medium; rapid results are assured (3).…”

Section: Methods and Proceduresmentioning

confidence: 99%