The Rapid Manual
            <i>Para</i>
            Sight™-F Test for Diagnosing
            <i>Plasmodium Falciparum</i>
            Malaria in Saudi Arabia

Omar, Mohamed S.; Malik, Ghada; Al-Amari, Omar M.; Abdalla, Salah E.; Moosa, Riyad A.

doi:10.5144/0256-4947.1999.159

Cited by 7 publications

(4 citation statements)

References 9 publications

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“…Though the significant progress achieved by malaria control program in Saudi Arabia the disease remains a serious health issue, particularly in endemic south-western regions where P. falciparum is the most predominant species accounting for over 90% of autochthonous cases, and patients with clinically suspected malaria signs are frequently encountered (Omar et al, 1999;Al-Harthi and Jamjoom, 2008). Also, an important number of imported malaria cases is being registered annually both, in endemic and non-endemic areas, among visitors coming for work or Ummrah and Hajj (Alkhalife, 2003;Al-Tawfiq, 2006).…”

Section: Discussionmentioning

confidence: 99%

Comparison of a Genus-Specific Conventional PCR and a Species-Specific Nested-PCR for Malaria Diagnosis Using FTA Collected Samples from Kingdom of Saudi Arabia

Al-Harthi

2015

JESP

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Molecular tools are increasingly accepted as the most sensitive and reliable techniques for malaria diagnosis and epidemiological surveys. Also, collection of finger prick blood spots onto filter papers is the most simple and affordable method for samples preservation and posterior molecular analysis, especially in rural endemic regions where malaria remains a major health problem. Two malaria molecular diagnostic tests, a Plasmodium genus-specific conventional PCR and a Plasmodium species-specific Nested PCR, were evaluated using DNA templates prepared from Whatman-FTA ®cards' dry blood spots using both, Methanol-fixation/Heat-extraction and FTA® commercial purification kit. A total of 121 blood samples were collected from six Saudi southwestern endemic districts both, as thick and thin films for routine microscopic screening and onto FTA®cards for molecular studies. Out of the 121 samples, 75 were P. falciparum positive by at least one technique. No other species of Plasmodium were detected. P. falciparum parasites were identified in 69/75 (92%) samples by microscopic screening in health care centers. P. genus-specific PCR was able to amplify P. falciparum DNA in 41/75 (55%) and 59/75 (79%) samples using Methanol-fixation/Heat-extraction and FTA ® purification kit, respectively. P. species-specific Nested PCR revealed 68/75 (91%) and 75/75 (100%) positive samples using DNA templates were isolated by Methanol-fixation/Heatextraction and FTA ® purification methods, respectively. The species-specific Nested PCR applied to Whatman-FTA ® preserved and processed blood samples represents the best alternative to classical microscopy for malaria diagnosis, particularly in epidemiological screening.

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Section: Discussionmentioning

confidence: 99%

Comparison of a Genus-Specific Conventional PCR and a Species-Specific Nested-PCR for Malaria Diagnosis Using FTA Collected Samples from Kingdom of Saudi Arabia

Al-Harthi

2015

JESP

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“…[1][2][3][4][5][6] In Saudi Arabia, malaria transmission is confined to southwestern regions (the Aseer and Jazan provinces), where P. falciparum is the prevailing species and Anopheles arabiensis is the main vector. [7][8][9][10][11] However, malaria cases are still reported in different parts of the country, mostly brought by travelers from endemic sites in the south or expatriates from outside the country. 1,12 Chloroquine was the drug of choice for the treatment of uncomplicated malaria cases for many years.…”

Section: Introductionmentioning

confidence: 99%

Distribution of Drug Resistance Genotypes in Plasmodium falciparum in an Area of Limited Parasite Diversity in Saudi Arabia

Dajem

Al-Farsi

Al-Hashami

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…10 Omar di Saudi Arabia memperoleh nilai sensitivitas 95-100% dan spesifisitas 100%, nilai prediksi positif 100% dan nilai prediksi negatif 91%. 11 Genton dkk tahun 1995 meneliti diagnosis pada infeksi P.falciparum dengan memakai uji ParaSight  dalam darah dan dalam urin pada anak-anak Papua New Guinea didapatkan hasil sensitivitas dalam darah 84% dari spesifisitas 77% dibandingkan dalam urin nilai sensitivitas sebesar 81% dan spesifisitas hanya 26%. Oleh karena spesifisitas dalam urin rendah sehingga tidak direkomendasikan uji ParaSight  dalam urin.…”

Section: Rapid Manual Testunclassified

Rapid Manual Test sebagai Alat Diagnostik Malaria falciparum

Desrinawati

2016

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Malaria adalah penyakit infeksi akut maupun kronis yang disebabkan olehDiagnosis malaria dapat ditegakkan dengan uji mikroskopis dan non mikroskopis. Uji mikroskopis dapat dilihat secara langsung di bawah mikroskop, seperti darah tepi, dan quantitative buffy coat (QBC). Sedangkan pada uji non mikroskopis, identifikasi antigen parasit atau antibodi antiplasmodial atau produksi metabolik parasit, salah satu contoh kitnya adalah rapid manual test. 5Tujuan tulisan ini adalah untuk mengetahui berbagai metode rapid manual test untuk diagnosis malaria P.falciparum secara cepat dan mudah. Rapid Manual TestRapid manual test merupakan uji yang lebih cepat, mudah dilakukan dan tidak memerlukan laboratorium khusus, seperti peralatan centrifuge dan miskroskop. Uji ini lebih praktis digunakan di lapangan. Diagnosis yang cepat dan akurat adalah kunci penanganan yang efektif untuk mengatasi penyakit malaria. Uji ini berdasarkan pada deteksi antigen yang dikeluarkan oleh parasit malaria yang dapat melisiskan darah, menggunakan alaria adalah penyakit infeksi akut maupun kronis yang disebabkan oleh Plasmodium malariae dengan gejala demam rekuren, anemia, dan hepatosplenomegali. 1Terdapat bukti bahwa penyakit ini mempunyai tingkat endemisitas yang tinggi di beberapa kawasan pemukiman di daerah tropis dan subtropis sejak masa prasejarah. Hingga akhir dari pertengahan abad kedua puluh, masih terjadi 250 juta kasus setiap tahun di seluruh dunia, dan sekitar 2,5 juta manusia meninggal oleh penyakit ini setiap tahunnya.2 Khususnya pada bayi dan anak, angka kematian dan kesakitan pada umur di bawah 5 tahun adalah 6%, dan 11 %, di Afrika 10% angka kematian pada anak disebabkan oleh malaria. 3,4 Di Indonesia, sampai saat ini penyakit malaria masih merupakan masalah kesehatan masyarakat.

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The Rapid Manual Para Sight™-F Test for Diagnosing Plasmodium Falciparum Malaria in Saudi Arabia

Cited by 7 publications

References 9 publications

Comparison of a Genus-Specific Conventional PCR and a Species-Specific Nested-PCR for Malaria Diagnosis Using FTA Collected Samples from Kingdom of Saudi Arabia

Comparison of a Genus-Specific Conventional PCR and a Species-Specific Nested-PCR for Malaria Diagnosis Using FTA Collected Samples from Kingdom of Saudi Arabia

Distribution of Drug Resistance Genotypes in Plasmodium falciparum in an Area of Limited Parasite Diversity in Saudi Arabia

Rapid Manual Test sebagai Alat Diagnostik Malaria falciparum

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