The rate of osmotic influx of water by flexible and inflexible erythrocytes

Sirs, John A.

doi:10.1113/jphysiol.1969.sp008957

Cited by 9 publications

(3 citation statements)

References 12 publications

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“…A comparison of the time dependences of the normalized conductance measured for different hypotonic solutions (figure 3(d)) indicates that the initial rate of swelling (dV/dt| t → 0 ∼ −(G max ) −1 dG/dt| t → 0 ) correlates negatively with the tonicity of a hypotonic solution. Qualitatively, these results are in line with the expression suggested by Sirs (1969):…”

Section: Resultssupporting

confidence: 90%

Erythrocyte swelling and membrane hole formation in hypotonic media as studied by conductometry

Pribush¹,

Meyerstein²,

Hatskelzon³

et al. 2013

Physiol. Meas.

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Hypoosmotic swelling of erythrocytes and the formation of membrane holes were studied by measuring the dc conductance (G). In accordance with the theoretical predictions, these processes are manifested by a decrease in G followed by its increase. Thus, unlike the conventional osmotic fragility test, the proposed methodological approach allows investigations of both the kinetics of swelling and the erythrocyte fragility. It is shown that the initial rate of swelling and the equilibrium size of the cells are affected by the tonicity of a hypotonic solution and the membrane rheological properties. Because the rupture of biological membranes is a stochastic process, a time-dependent increase in the conductance follows an integral distribution function of the membrane lifetime. The main conclusion which stems from reported results is that information about rheological properties of red blood cell (RBC) membranes and the resistivity of RBCs to a certain osmotic shock may be extracted from conductance signals.

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Section: Resultssupporting

confidence: 90%

Erythrocyte swelling and membrane hole formation in hypotonic media as studied by conductometry

Pribush¹,

Meyerstein²,

Hatskelzon³

et al. 2013

Physiol. Meas.

View full text Add to dashboard Cite

show abstract

“…Despite extensive changes of the membrane protein structure, the protein fixation did not change the diffusion permeability . A similar treatment with formaldehyde did not change osmotic water permeability (Sirs, 1969) . It is also unlikely that the anion transport pathway of the integral membrane proteins concomitantly offers a nonspecific leak pathway to water and small nonelectrolytes, as suggested by Brown et al (1975), because the specific anion transport inhibitor DIDS and the nonspecific inhibitor of facilitated transport processes phloretin both inhibit anion transport >99%, whereas water permeability remained unaffected .…”

Section: Inhibition Of Diffusional Water Permeabilitymentioning

confidence: 83%

“…As discussed below, this could be caused by two opposing effects : inhibition ofwater transport through proteins, and an increase of permeability through the lipids by a liquefying effect . An increase of water permeability through the lipids of the membrane could not, however, be produced by aliphatic alcohols at concentrations that increase alcohol permeability in red cells (Brahm, 1982), and water and electrolyte permeability in artificial membrane systems (Jain et al ., 1973;Hochster and Prestegard, 1977; Gut- (Sirs, 1969) in medium B containing 2 mM CaC12.…”

Section: Dependence On Osmolarity and Cell Volumementioning

confidence: 99%