The Rate of Transcapillary Exchange of Sodium in Normal and Shocked Dogs

Gellhorn, Alfred; Merrell, Margaret; Rankin, Robert M.

doi:10.1097/00005053-194505000-00016

Cited by 5 publications

(6 citation statements)

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“…This conclusion was based on the virtual lack of change in the "plateau" reading of Figure 1 when the counter was moved in a circle with the liver as center (Curves D, E, and F of Figure 1). (29,30,32). Figure 2 indicates that much of the radiomanganese initially in the blood is cleared within a minute of virtual mixing, with very little remaining by 45 minutes.…”

Section: A the Dynamic Metabolic State Of Manganesementioning

confidence: 99%

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Manganese Metabolism in Man: Rapid Exchange of MN56 with Tissue as Demonstrated by Blood Clearance and Liver Uptake1

Borg¹,

Cotzias²

1958

J. Clin. Invest.

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Despite the well-known physiological importance of manganese (1-7) and its implication in some disease states (2,(8)(9)(10), the exact nature of its metabolic role remains undefined (11,12).In mammals manganese concentrates in organs rich in mitochondria, notably the liver (2-7, 11, 13, 14); and previous work from this group (11) has shown these organelles to be the major pool of radiomanganese turnover shortly after injection into animals. In vitro traces of the element have widespread biochemical activity (2,3,(15)(16)(17)(18)(19), especially in relationship to mitochondria (20) and those of their enzyme systems that carry out oxidative phosphorylation (16,(21)(22)(23)(24)(25)(26).The present study of blood clearance and of radiation measured at the body surface following Mn" injection in man is an attempt to describe and to determine the kinetics of this turnover, which appears to correlate with mitochondrial function. From the analysis of these data in relation to the work of others it is also possible to gain insight into the mechanisms of transport directly from the blood. Our results further suggest that most of the normal body manganese is readily exchangeable and is not bound in metalloproteins or other highly stable configurations. EXPERIMENTAL Radioisotopic tracer. Mn" (half-life: 2.58 hr.) 2 was generated in the Brookhaven research reactor. One ml. aliquots containing 11.1 ,g. of MnSOgH2O (3.6 ,ug. of Mn' 3 were injected into patients. The radiation decay of other aliquots was followed for 48 to 72 hours with a well-type NaI(Ti) scintillating crystal and scaling circuit. The resultant activity curves indicated the only contaminating radioelement to be a small amount of Na' (about 0.5 per cent of the Mn' activity at injection time), which was ignored in the subsequent treatment of the data.Route of administration and withdrawal of blood samples. Following an overnight fast, patients received Mn" tracer intravenously. Thereafter serial venous blood samples of approximately 3 ml. volume were withdrawn from the contralateral arm. Samples were promptly assayed for radioactivity and weighed. Corrections for radioactive decay were then made. Frequent samples were obtained during the first few minutes after injection, but later sampling frequency was decreased gradually to intervals of approximately five minutes. Surface counting technique. Concurrently with thewithdrawal of blood samples, radiation was monitored over the body surface with collimated detectors whose outputs were transcribed automatically.4 As anticipated from animal data (2, 4-7, 11), preliminary scanning following Mn' injection revealed localization of the greatest radioactivity in the region of the liver. Accordingly, one scanner was positioned on the skin surface over the liver area, usually about 3 cm. above the costal margin in the right midclavicular line ("liver area" counter). A second counter was located over the right femur about 18 cm. above the patella ("thigh" counter). This was taken to be a "reference area" that would not...

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Section: A the Dynamic Metabolic State Of Manganesementioning

confidence: 99%

“…with a. equal to the sum of a, (29,32). After substitution from Equation 1, R becomes about 69 per cent.…”

Section: A the Dynamic Metabolic State Of Manganesementioning

confidence: 99%

Manganese Metabolism in Man: Rapid Exchange of MN56 with Tissue as Demonstrated by Blood Clearance and Liver Uptake1

Borg¹,

Cotzias²

1958

J. Clin. Invest.

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“…3 and demonstrate the points made in this section. A double exponential can result from a parallel arrangement of two compartments of tissue potassium (Gellhorn, Merrell & Rankin, 1944). Provided that potassium exchange was complete at the beginning of efflux, then a and b are the sizes of the potassium fractions exchanging with rate constants k1 and k2, respectively.…”

Section: Value Of a And Bmentioning

confidence: 99%

A comparison of methods for measuring efflux of labelled potassium from contracting rabbit atria

Persoff¹

1960

The Journal of Physiology

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“…In the dog, Gellhorn et al (12) were unable to obtain reliable results by the use of this method, a conclusion supported by the preliminary experiments in man of Sweet, Solomon, and Selverstone (15). This may be due to incomplete knowledge about the early part of the curve.…”

Section: Case 3-closed Two-compartment Systemmentioning

confidence: 93%