The Ratio of Linoleic to Oleic Acid in Sunflower Oil, and its Effects on Bacterial Growth

Abstract

This study aims to explore the extent to which varying the ratio of linoleic to oleic acid in processed sunflower affects the growth of S. epidermidis and E. coli. A modified Kirby-Bauer test was utilized to obtain quantitative results. Sunflower oil was diluted to various concentrations using Tween 80, then applied to bacteria inoculated in either pour-plate or spread-plate methods. All conditions tested were done in triplicate to conform to standards in antibacterial susceptibility testing.