The ratio of urinary TREM-1/TREM-2 mRNA expression in chronic kidney disease and renal fibrosis

Cao, Yuhan; Wang, Yuwei; Peng, Nana; Xiao, Jie; Wang, Sufen; Fu, Cong

doi:10.1080/07853890.2021.1912384

Cited by 7 publications

(8 citation statements)

References 24 publications

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“…firstly established a non-invasive approach to diagnosing acute renal rejection of allografts by isolating and quantifying RNA of specific genes in urine cells [ 4 ]. Urinary sediment had attracted researchers' focus and had become an attractive resource for detecting the biomarker for kidney diseases [ 11 , 14–17 ]. However, the current urine cell mRNA detection technology still had some limitations.…”

Section: Discussionmentioning

confidence: 99%

“…The degree of renal fibrosis for CKD patients was evaluated and the Score of TIF and glomerular sclerosis were calculated according to the previous study described [ 11 ]. Two experienced pathologists who were blinded to the studies subjectively scored the severity of renal fibrosis.…”

Section: Methodsmentioning

confidence: 99%

“…SPSS 17.0 was used for data analysis. The method of calculating the relative expression of hsa_circ_0036649 was described in a previous study [ 11 ]. Statistical comparison of different types of data, calculate the correlation coefficient, logistic regression and receiver operating characteristic (ROC) curves were calculated according to the previous study described [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

“…The method of calculating the relative expression of hsa_circ_0036649 was described in a previous study [ 11 ]. Statistical comparison of different types of data, calculate the correlation coefficient, logistic regression and receiver operating characteristic (ROC) curves were calculated according to the previous study described [ 11 ]. The P value of the deviation of linearity was calculated to confirm the linear relationship between expression of hsa_circ_0003649 and scr, BUN, cycstatin C, eGFR, 24 h proteinuria, a score of TIF and score of glomerular sclerosis ( p > .05 indicated the linear relationship).…”

Section: Methodsmentioning

confidence: 99%

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Urinary exosomes derived circRNAs as biomarkers for chronic renal fibrosis

Cao

Shi²,

Yang³

et al. 2022

Annals of Medicine

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Background Chronic renal disease (CKD) is a common and irreversible loss of renal function. Renal fibrosis reflected the degree of renal dysfunction. However, the current biomarkers only characterize the renal function instead of indicating the fibrosis degree. The potential diagnostic value of urinary exosomes derived circRNAs for renal fibrosis needs to be further studied. Methods Urine exosomes from 3 chronic kidney disease (CKD) patients without renal fibrosis and 3 renal fibrotic patients were collected and human circRNAs microarray analysis were performed to detect the circRNAs expression profile. 110 biopsy-proven CKD patients and 54 healthy controls were enrolled and urine exosomes derived RNA was isolated. The expression of hsa_circ_0036649 was measured and the correlation with renal function parameter and pathological indicators was performed. The receiver operating characteristic (ROC) curve for the diagnosis of renal fibrosis was calculated. Results Human circRNAs microarray showed 365 circRNAs up expressed and 195 circRNAs down expressed in renal fibrotic patients compared to none fibrosis CKD patients. The expression of hsa_circ_0036649 was decreased in renal fibrotic patients according to RT-PCR and correlated with serum creatinine, blood urea nitrogen (BUN), estimated glomerular filtration rate and cystatin c. Further, the expression of hsa_circ_0036649 was correlated with the score of tubulointerstitial fibrosis (TIF) and the score of glomerular sclerosis. The ROC curve showed that hsa_circ_0036649 may predict renal fibrosis at a cut-off value of 0.597 with a sensitivity of 45.5% and specificity of 87.9%. Conclusion Expression of urinary exosomes derived hsa_circ_0036649 associated with the degree of renal fibrosis. Its potential role as a biomarker in CKD remained to be supported by further follow-up studies. Key Messages circRNAs profile in urine exosomes in renal fibrosis patients was revealed. The expression of urine exosomes derived hsa_circ_0036649 was correlated to renal function and fibrosis degree. circRNAs derived from urinary exosomes may become a new research direction for biomarkers of renal fibrosis.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Urinary exosomes derived circRNAs as biomarkers for chronic renal fibrosis

Cao

Shi²,

Yang³

et al. 2022

Annals of Medicine

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“…A 2018 review summarised literature investigating transcriptomic analysis in the context of kidney disease (23), with a number of additional investigations published in recent years (146,(148)(149)(150)(151)(152)(153)(154)(155)(156)(157)(158)(159). An interesting study by Fan et al carried out RNA-seq analysis of kidney biopsies from early DKD, advanced DKD, or control patients, to reveal gene expression changes from healthy to disease states (158).…”

Section: Emerging Insights From Transcriptomic Analysis Of Ckdmentioning

confidence: 99%

Harnessing the Full Potential of Multi-Omic Analyses to Advance the Study and Treatment of Chronic Kidney Disease

Hill¹,

Ávila-Palència²,

Maxwell³

et al. 2022

Front. Nephrol.

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Chronic kidney disease (CKD) was the 12th leading cause of death globally in 2017 with the prevalence of CKD estimated at ~9%. Early detection and intervention for CKD may improve patient outcomes, but standard testing approaches even in developed countries do not facilitate identification of patients at high risk of developing CKD, nor those progressing to end-stage kidney disease (ESKD). Recent advances in CKD research are moving towards a more personalised approach for CKD. Heritability for CKD ranges from 30% to 75%, yet identified genetic risk factors account for only a small proportion of the inherited contribution to CKD. More in depth analysis of genomic sequencing data in large cohorts is revealing new genetic risk factors for common diagnoses of CKD and providing novel diagnoses for rare forms of CKD. Multi-omic approaches are now being harnessed to improve our understanding of CKD and explain some of the so-called ‘missing heritability’. The most common omic analyses employed for CKD are genomics, epigenomics, transcriptomics, metabolomics, proteomics and phenomics. While each of these omics have been reviewed individually, considering integrated multi-omic analysis offers considerable scope to improve our understanding and treatment of CKD. This narrative review summarises current understanding of multi-omic research alongside recent experimental and analytical approaches, discusses current challenges and future perspectives, and offers new insights for CKD.

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Urinary single-cell sequence analysis of the urinary macrophage in different outcomes of membranous nephropathy

Zhao

Niu

et al. 2023

Clinical Kidney Journal

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Background Great progresses have been made in diagnosis and treatment of membranous nephropathy (MN). However, a significant number of patients do not respond to immunosuppressive therapy and eventually progress to ESRD. To investigate the mechanism of different outcome of MN, we performed single-cell sequencing to analysis the urine cells of patients with and without complete remission of MN. Methods Urine single-cell RNA sequencing was performed on 12 healthy controls (HC) and 15 patients with MN. The patients were divided into complete remission group (CR, n = 9) and no remission group (NR, n = 6). Results 1) Macrophages were the largest group in urine cells, they were 48.02%, 68.96% and 20.95% in the HC, CR and NR group, respectively. 2) Urinary macrophages expressing FIColin-1 and S100 calcium binding protein A8 were mainly in HC group and CR group, indicating that they were derived from bone marrow and peripheral blood, while the urinary macrophages expressing the regulator of G-protein signaling 1 and HLA-DPA1, mainly in the NR group, were derived from renal resident macrophages. 3) In healthy adults, urine macrophages expressed the metallothionein family, indicating that they can regulate anti-inflammatory and proinflammatory functions bidirectionally. In the CR group, the urine macrophages showed strong proinflammatory properties. In the NR group, the urinary macrophages mainly associated with the level of proteinuria and the impaired renal function. Conclusions Our study firstly delineated the differences of urinary cell maps between healthy individuals and the MN patients with CR or NR outcomes. Not only in origin but also in function of urine macrophages were different in the HC, CR and NR group.

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The ratio of urinary TREM-1/TREM-2 mRNA expression in chronic kidney disease and renal fibrosis

Cited by 7 publications

References 24 publications

Urinary exosomes derived circRNAs as biomarkers for chronic renal fibrosis

Urinary exosomes derived circRNAs as biomarkers for chronic renal fibrosis

Harnessing the Full Potential of Multi-Omic Analyses to Advance the Study and Treatment of Chronic Kidney Disease

Urinary single-cell sequence analysis of the urinary macrophage in different outcomes of membranous nephropathy

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