The reactions between glutaraldehyde and various proteins. An investigation of their kinetics

Hopwood, David A.; Allen, C. R.; McCabe, Michelle

doi:10.1007/bf01003541

Cited by 111 publications

(69 citation statements)

References 23 publications

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“…EDCs can alter or inhibit the function of the endocrine system by binding to estrogen receptors, which are part of the nuclear receptor superfamily [47][48][49]. Studies have shown that the nuclear receptor PXR binds to certain EDCs with different affinities [50,51]. Figure 4 shows the nanomechanical response magnitude of PXR functionalized MCs on exposure to 1.0 M EDCs, phthalic acid, nonylphenol, and bisphenol A (in 10 mM PBS, pH 7.0).…”

Section: Detection Of Known Pxr Activators Andmentioning

confidence: 99%

Microcantilever-based Nanomechanical Studies of the Orphan Nuclear Receptor Pregnane X Receptor-Ligand Interactions

Hill¹,

Dutta²,

Zhang³

2011

JBNB

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Human pregnane X receptor (PXR) is of vital importance in pharmaceutical and exogenous compound metabolism within the body. This provides strong motivation for investigating this orphan receptor's activation by various pharmaceuticals, xenobiotics, and endocrine disrupting chemicals (EDCs). A nanomechanical transducer is developed to study xenobiotic and EDC interactions with the bioreceptor PXR's ligand binding domain (LBD). The combination of immobilized LBD PXR with a nanostructured microcantilever (MC) platform allows for the sensitive, label-free study of ligand interaction with the receptor. PXR shows real-time, reversible responses when exposed to a specific pharmaceutical, EDCs, and xenobiotic ligands. Three EDCs binding interactions are tested, which include phthalic acid, nonylphenol, and bisphenol A, with PXR. PXR LBD was exposed to rifampicin, a potent PXR activator, with various injection and recovery times to study their interaction. A two protein array of PXR and estrogen receptor  (ER-) directlycompares the nanomechanical responses of these receptors with rifampicin on a single platform.

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Section: Detection Of Known Pxr Activators Andmentioning

confidence: 99%

Microcantilever-based Nanomechanical Studies of the Orphan Nuclear Receptor Pregnane X Receptor-Ligand Interactions

Hill¹,

Dutta²,

Zhang³

2011

JBNB

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“…When comparing GTA virucidal activity against isolates and reference strains of poliovirus 1 and echovirus 25, we also observed significant intertypic and intratypic differences (6,7). Numerous reports investigating the process of binding between GTA and various proteins and enzymes have shown that only the ε-amino group of lysine reacts to any significant extent with GTA (8,16,17,20). In light of these findings, we made the assumption that the differences previously observed between enterovirus strains were related to variations in the locations of lysine residues in the capsid proteins, in particular in externalized regions of the VP1 protein.…”

mentioning

confidence: 96%

Virucidal Efficacy of Glutaraldehyde against Enteroviruses Is Related to the Location of Lysine Residues in Exposed Structures of the VP1 Capsid Protein

Chambon

Archimbaud

Bailly

et al. 2004

Appl Environ Microbiol

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Glutaraldehyde (GTA) is a potent virucidal disinfectant whose exact mode of action against enteroviruses is not understood. Earlier reports showed that GTA reacts preferentially with the VP1 capsid protein of echovirus 25 and poliovirus 1 and that GTA has affinity for exposed lysine residues on proteins. To investigate further the inactivation of enteroviruses by GTA, seven strains were selected on the basis of differences in their overall number and the positions of lysine residues in the amino acid sequences of the VP1 polypeptide. Inactivation kinetics experiments were performed with 0.10% GTA. The viruses grouped into three clusters and exhibited significantly different levels of sensitivity to GTA. The results were analyzed in the light of current knowledge of the three-dimensional structure of enteroviruses and the viral life cycle. The differences observed in sensitivity to GTA were related to the number of lysine residues and their locations in the VP1 protein. The overall findings suggest that the BC and DE loops, which cluster at the fivefold axis of symmetry and are the most exposed on the outer surface of the virions, are primary reactive sites for GTA.

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“…Also for other proteins gluteraldehyde has been reported as the most efficient cross-linking agent yielding thermally and chemically stable cross-links (Bowes & Cater, 1968;Nimni et al, 1987). Primary amino groups have been reported to act as prime target to initiate the aldehydeinduced cross-linking reaction (Quiocho & Richards, 1966), next to aromatic amino acids (Hopwood et al, 1970). Depending on environmental conditions, gluteraldehyde can bring about cross-linking through a wide variety of reaction mechanisms (reviewed in Migneault et al, 2004).…”

Section: Cross-linkingmentioning

confidence: 99%

Application Potential of Food Protein Modification

Jongh¹,

Broersen²

2012

Advances in Chemical Engineering

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The reactions between glutaraldehyde and various proteins. An investigation of their kinetics

Cited by 111 publications

References 23 publications

Microcantilever-based Nanomechanical Studies of the Orphan Nuclear Receptor Pregnane X Receptor-Ligand Interactions

Microcantilever-based Nanomechanical Studies of the Orphan Nuclear Receptor Pregnane X Receptor-Ligand Interactions

Virucidal Efficacy of Glutaraldehyde against Enteroviruses Is Related to the Location of Lysine Residues in Exposed Structures of the VP1 Capsid Protein

Application Potential of Food Protein Modification

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