The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria

Nordmann, Patrice; Cuzon, Gaëlle; Naas, Thierry

doi:10.1016/s1473-3099(09)70054-4

Cited by 1,392 publications

(1,268 citation statements)

References 76 publications

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“…The utility of the boronic acid compounds have recently been established, standing out as efficient inhibitors that can be used in disk potentiation tests, to differentiate KPC producers from those producing extended-spectrum beta lactamase [10,22]. However, it is important to note that there are different boronic acid compounds and different concentrations that are used.…”

Section: Discussionmentioning

confidence: 99%

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The combined-disk boronic acid test as an accurate strategy for the detection of KPC carbapenemase in Central America

Zúñiga

Cruz

Pérez

et al. 2016

J Infect Dev Ctries

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Introduction: Carbapenemase-producing Klebsiella pneumoniae (KPC) outbreaks may cause a huge economical burden on developing countries. Furthermore, KPC can be challenging to detect. We describe the laboratory strategy for the detection of KPC from 2011 to 2013 in a tertiary care hospital in Central America with approximately 1,000 beds. Methodology: A retrospective analysis of a clinical laboratory database was done to determine the pragmatic application of the combined-disk boronic acid test during a KPC outbreak in Panama. A total of 1,026 Klebsiella pneumoniae isolates were found, of which 133 were positive for KPC. The strategy during two phases was described according to the test employed as a confirmatory test for KPC. After the K. pneumoniae isolates were detected by the VITEK 2 system, blaKPC polymerase chain reaction (PCR) and the combined-disk boronic acid test were employed as a confirmatory test during phase one. The combined-disk boronic acid test was employed as a confirmatory test for KPC during phase two. Results: The sensitivity, specificity, positive predictive value, and negative predictive value of the boronic acid test were 100%, 97%, 91%, and 100%, respectively, when blaKPC PCR was employed as a confirmatory test during the start of the outbreak. Afterwards, modified VITEK 2 system parameters resulted in 116 suspicious KPC samples and the boronic acid test confirmed 102 isolates. Conclusions: The use of an automated bacterial identification system and the boronic acid test for the detection of KPC was an effective and low-cost strategy for a clinical laboratory in Panama during an outbreak.

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Section: Discussionmentioning

confidence: 99%

“…The initial detection through disk diffusion agar remains difficult, and automated bacterial identification systems have low diagnostic accuracy [10,11]. Several tests have been employed to detect KPC, including the Hodge test, the modified Hodge test, and the boronic acid test [9,12].…”

Section: Introductionmentioning

confidence: 99%

The combined-disk boronic acid test as an accurate strategy for the detection of KPC carbapenemase in Central America

Zúñiga

Cruz

Pérez

et al. 2016

J Infect Dev Ctries

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“…Routine antibiotic susceptibility tests are not sufficient to detect KPC-producing bacteria. Therefore, infection control policies for controlling the spread of these pathogens usually fail (26). Different types of metallo-β-lactamases dissemination in Iran are presented in Table 1.…”

Section: Klebsiella Pneumoniae Carbapenemasementioning

confidence: 99%

Laboratory Detection of Carbapenemases in Gram-Negative Bacteria

Goudarzi

Taherpour

Fallah

et al. 2016

Arch Clin Infect Dis

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The nosocomial infections, caused by multi-drug resistant bacteria, are the most important cause of mortality throughout the world. One important mechanism against beta-lactam antibiotics is the production of beta-lactamases. Enterobacteriaceae and nonfermentative bacteria, like Pseudomonas aeruginosa and Acinetobacter baumannnii may produce these enzymes. Currently, there is no vaccine to prevent the infections caused by β-lactamase-producing bacteria. Consequently, it is necessary to identify β-lactamaseproducing bacteria by phenotypic and molecular methods.

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“…Desde su primera detección en Klebsiella pneumoniae en 1996 (16), las KPC se han reportado como causantes de epidemias dentro y fuera de Estados Unidos (17)(18)(19)(20)(21)(22)(23). En Colombia, el primer reporte de una KPC se hizo en K. pneumoniae en 2006 (14) y en 2007 se reportó por primera vez en el mundo en Pseudomonas aeruginosa (17).…”

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Prevalencia de bacterias Gram negativas portadoras del gen blaKPC en hospitales de Colombia

et al. 2013

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34(Supl.1):81-90 Prevalencia de bacterias portadoras de blaKPC en Colombia Contribución de los autores:Robinson Pacheco: realización del estudio, recolección de la información clínica y epidemiológica, realización de los análisis estadísticos y redacción del manuscrito. Lyda Osorio: participación en el diseño del estudio, supervisión de la recolección de información y del análisis estadístico. Adriana M. Correa: coordinación de las actividades de laboratorio, desarrollo de la técnica molecular de PFGE. María Virginia Villegas: participación en el diseño y coordinación general del estudio. Todos los autores participaron en la revisión y corrección del manuscrito. ARTÍCULO ORIGINAL Biomédica 2014;34(Supl.

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The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria

Cited by 1,392 publications

References 76 publications

The combined-disk boronic acid test as an accurate strategy for the detection of KPC carbapenemase in Central America

The combined-disk boronic acid test as an accurate strategy for the detection of KPC carbapenemase in Central America

Laboratory Detection of Carbapenemases in Gram-Negative Bacteria

Prevalencia de bacterias Gram negativas portadoras del gen blaKPC en hospitales de Colombia

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