The Rebinding Properties of Bovine Serum Albumin Imprinted Calcium Alginate/Phosphate Hybrid Microspheres Via the Adjustment of pH Values and Salt Concentration

Abstract

Summary: Bovine serum albumin imprinted calcium phosphate/alginate hydrogel microspheres were prepared with sodium alginate (SA), (NH4)2HPO4, and using CaCl2 as gelling agent, bovine serum albumin (BSA) as template in inverse suspension. The optimized rebinding properties of BSA imprinted hydrogel microspheres were investigated by controlling pH value and ionic concentration from the viewpoint of adjusting the process of gelling, removing template and rebinding. The optimized pH values for the imprinting of BSA in gelling, removing template and rebinding process was 4.1, 8.3 and 4.8, respectively. The effect of NaCl concentration on the BSA rebinding was also determined. We provided a strategy to get the optimized imprinting efficiency by altering pH value and ionic concentration in a weakly ionic cross‐linked hydrogel system on the process of protein's imprinting.

Protein‐imprinted polyurethane‐grafted calcium alginate hydrogel microspheres

“…and Herrero and coworkers prepared bovine serum albumin (BSA) imprinted calcium alginate (CaA) hydrogel microspheres under mild circumstances. Zhao et al . prepared BSA‐imprinted CaA/phosphate hybrid microspheres.…”

Molecularly Imprinted Polymers with Stimuli-Responsive Affinity: Progress and Perspectives

“…Similarly, Oral et al reported pH-responsive and recognitive polymeric networks based on PEG star polymers to distinguish D-glucose from similar sugars by using methylacryl acid (MAA) as the ionizable comonomer [104]. In addition, equilibrium swelling and rebinding properties of BSA imprinted hybrid hydrogel microspheres based on calcium alginate and phosphate were also evaluated in different pH values and even ionic concentrations [105]. Recently, this type of pH-responsive imprinted hydrogels was applied as horseradish peroxidase mimetic enzymes.…”

“…imprinted hybrid hydrogel microspheres based on calcium alginate and phosphate were also evaluated in different pH values and even ionic concentrations [105]. Recently, this type of pH-responsive imprinted hydrogels was applied as horseradish peroxidase mimetic enzymes.…”