The recent advances of high-throughput biosensors for rapid detection of foodborne pathogens

Zhao, Yibing; Shang, Yuting; Wang, Zhengzheng; Wang, Zhi; Xie, Jihang; Zhai, Huichan; Huang, Zhenying; Wang, Yantao; Wu, Qingping; Ding, Yu; Wang, Juan

doi:10.1016/j.trac.2024.117736

Cited by 3 publications

(2 citation statements)

References 199 publications

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“…63,64 Furthermore, the advantages of immunoassay in high-throughput detection mainly lie in high-throughput detection capability, high sensitivity and specificity, multi parameter detection, rapid response, automated operation, wide application range, and data analysis and interpretation. 65 However, since most marine biotoxin groups are composed of a large number of homologues, there may be large differences in binding affinity between homologues and antibodies. 66 If a homologue has high toxicity and little cross-reactivity, the results of the immunoassay may not truly reflect the toxicity of the samples.…”

Section: Immunoassay For Marine Biotoxinsmentioning

confidence: 99%

“…The immunoassay technique is based on the highly selective reaction between antibodies and antigens with high sensitivity, and it is amenable to high-throughput screening of samples in a short period, making it an effective tool for marine biotoxin detection. , Furthermore, the advantages of immunoassay in high-throughput detection mainly lie in high-throughput detection capability, high sensitivity and specificity, multi parameter detection, rapid response, automated operation, wide application range, and data analysis and interpretation . However, since most marine biotoxin groups are composed of a large number of homologues, there may be large differences in binding affinity between homologues and antibodies .…”

Section: Immunoassay For Marine Biotoxinsmentioning

confidence: 99%

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Toward Sensitive and Reliable Immunoassays of Marine Biotoxins: From Rational Design to Food Analysis

Ji,

Wang,

Zhao

2024

J. Agric. Food Chem.

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Marine biotoxins are metabolites produced by algae that can accumulate in shellfish or fish and enter organisms through the food chain, posing a serious threat to biological health. Therefore, accurate and rapid detection is an urgent requirement for food safety. Although various detection methods, including the mouse bioassay, liquid chromatography−mass spectrometry, and cell detection methods, and protein phosphatase inhibition assays have been developed in the past decades, the current detection methods cannot fully meet these demands. Among these methods, the outstanding immunoassay virtues of high sensitivity, reliability, and low cost are highly advantageous for marine biotoxin detection in complex samples. In this work, we review the recent 5-year progress in marine biotoxin immunodetection technologies such as optical immunoassays, electrochemical immunoassays, and piezoelectric immunoassays. With the assistance of immunoassays, the detection of food-related marine biotoxins can be implemented for ensuring public health and preventing food poisoning. In addition, the immunodetection technique platforms including lateral flow chips and microfluidic chips are also discussed. We carefully investigate the advantages and disadvantages for each immunoassay, which are compared to demonstrate the guidance for selecting appropriate immunoassays and platforms for the detection of marine biotoxins. It is expected that this review will provide insights for the further development of immunoassays and promote the rapid progress and successful translation of advanced immunoassays with food safety detection.

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Section: Immunoassay For Marine Biotoxinsmentioning

confidence: 99%

Section: Immunoassay For Marine Biotoxinsmentioning

confidence: 99%

Toward Sensitive and Reliable Immunoassays of Marine Biotoxins: From Rational Design to Food Analysis

Ji,

Wang,

Zhao

2024

J. Agric. Food Chem.

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Unsupervised Learning‐Assisted Acoustic‐Driven Nano‐Lens Holography for the Ultrasensitive and Amplification‐Free Detection of Viable Bacteria

Zhou,

Zhao,

Wen

et al. 2024

Advanced Science

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Bacterial infection is a crucial factor resulting in public health issues worldwide, often triggering epidemics and even fatalities. The accurate, rapid, and convenient detection of viable bacteria is an effective method for reducing infections and illness outbreaks. Here, an unsupervised learning–assisted and surface acoustic wave–interdigital transducer‐driven nano‐lens holography biosensing platform is developed for the ultrasensitive and amplification‐free detection of viable bacteria. The monitoring device integrated with the nano‐lens effect can achieve the holographic imaging of polystyrene microsphere probes in an ultra‐wide field of view (∽28.28 mm2), with a sensitivity limit of as low as 99 nm. A lightweight unsupervised learning hologram processing algorithm considerably reduces training time and computing hardware requirements, without requiring datasets with manual labels. By combining phage–mediated viable bacterial DNA extraction and enhanced CRISPR–Cas12a systems, this strategy successfully achieves the ultrasensitive detection of viable Salmonella in various real samples, demonstrating enhanced accuracy validated with the qPCR benchmark method. This approach has a low cost (∽$500) and is rapid (∽1 h) and highly sensitive (∽38 CFU mL−1), allowing for the amplification‐free detection of viable bacteria and emerging as a powerful tool for food safety inspection and clinical diagnosis.

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Progress in Separation and Detection of Foodborne Bacteria for Food Safety

Wang,

Jia,

et al. 2024

Current Opinion in Food Science

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The recent advances of high-throughput biosensors for rapid detection of foodborne pathogens

Cited by 3 publications

References 199 publications

Toward Sensitive and Reliable Immunoassays of Marine Biotoxins: From Rational Design to Food Analysis

Toward Sensitive and Reliable Immunoassays of Marine Biotoxins: From Rational Design to Food Analysis

Unsupervised Learning‐Assisted Acoustic‐Driven Nano‐Lens Holography for the Ultrasensitive and Amplification‐Free Detection of Viable Bacteria

Progress in Separation and Detection of Foodborne Bacteria for Food Safety

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