The Receptor Binding Domain of the New Middle East Respiratory Syndrome Coronavirus Maps to a 231-Residue Region in the Spike Protein That Efficiently Elicits Neutralizing Antibodies

Mou, Huihui; Raj, V. Stalin; Kuppeveld, Frank J. M. van; Rottier, Peter J. M.; Haagmans, Bart L.; Bosch, Berend Jan

doi:10.1128/jvi.01277-13

Cited by 221 publications

(227 citation statements)

References 25 publications

Supporting

Mentioning

209

Contrasting

Unclassified

Order By: Relevance

“…4E and F). This last observation is consistent with the fact that besides the RBD, other parts of the spike protein are able to induce neutralizing antibodies (17).…”

supporting

confidence: 76%

Middle East Respiratory Syndrome Coronavirus Spike Protein Delivered by Modified Vaccinia Virus Ankara Efficiently Induces Virus-Neutralizing Antibodies

Song¹,

Fux²,

Provacia

et al. 2013

J Virol

Self Cite

135

153

View full text Add to dashboard Cite

“…4E and F). This last observation is consistent with the fact that besides the RBD, other parts of the spike protein are able to induce neutralizing antibodies (17).…”

supporting

confidence: 76%

Middle East Respiratory Syndrome Coronavirus Spike Protein Delivered by Modified Vaccinia Virus Ankara Efficiently Induces Virus-Neutralizing Antibodies

Song¹,

Fux²,

Provacia

et al. 2013

J Virol

Self Cite

135

153

View full text Add to dashboard Cite

“…During the submission and review of the present study, two other studies independently mapped the MERS-CoV RBD fragments (residues 358 to 588 and 377 to 662), both of which are similar to the one identified in the present study (residues 367 to 588) (42,43). These studies also showed that the RBD efficiently elicits neutralizing antibodies, confirming that the crystal structure of MERS-CoV can be useful in structure-based vaccine design.…”

Section: Addendumsupporting

confidence: 57%

Crystal Structure of the Receptor-Binding Domain from Newly Emerged Middle East Respiratory Syndrome Coronavirus

Chen

Rajashankar

Yang

et al. 2013

J Virol

118

123

View full text Add to dashboard Cite

The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) has infected at least 77 people, with a fatality rate of more than 50%. Alarmingly, the virus demonstrates the capability of human-to-human transmission, raising the possibility of global spread and endangering world health and economy. Here we have identified the receptor-binding domain (RBD) from the MERS-CoV spike protein and determined its crystal structure. This study also presents a structural comparison of MERS-CoV RBD with other coronavirus RBDs, successfully positioning MERS-CoV on the landscape of coronavirus evolution and providing insights into receptor binding by MERS-CoV. Furthermore, we found that MERS-CoV RBD functions as an effective entry inhibitor of MERS-CoV. The identified MERS-CoV RBD may also serve as a potential candidate for MERS-CoV subunit vaccines. Overall, this study enhances our understanding of the evolution of coronavirus RBDs, provides insights into receptor recognition by MERS-CoV, and may help control the transmission of MERS-CoV in humans. Since the summer of 2012, a novel coronavirus, Middle East respiratory syndrome coronavirus (MERS-CoV), has emerged from the Middle East and spread to parts of Europe. MERS-CoV infection often leads to acute pneumonia and renal failure, and the human fatality rate is more than 50% (1, 2). To date, MERS-CoV has infected at least 77 people and was able to be transmitted from human to human. The genomic sequence of MERS-CoV is closely related to the sequences of certain bat coronaviruses (3-5), raising concerns over persistent bat-to-human cross-species transmission of the virus. The clinical signs and epidemic patterns of MERS-CoV are reminiscent of the severe acute respiratory syndrome coronavirus (SARS-CoV), the etiological agent of the worldwide SARS epidemic in 2002-2003 that infected more than 8,000 people with a ϳ10% fatality rate (6, 7). MERS-CoV poses a significant threat to global health and economy.Coronaviruses are enveloped and positive-stranded RNA viruses and can be divided into three major genera, ␣, ␤, and ␥ (8). They mainly cause respiratory, gastrointestinal, and central nervous system diseases in mammals and birds. Coronaviruses recognize a variety of host receptors. Human NL63 respiratory coronavirus (HCoV-NL63) from ␣-genus and SARS-CoV from ␤-genus both recognize angiotensin-converting enzyme 2 (ACE2) as their host receptor (9, 10). Porcine respiratory coronavirus (PRCV) and some other coronaviruses from ␣-genus recognize aminopeptidase N (APN) (11, 12). Mouse hepatitis coronavirus (MHV) from ␤-genus recognizes carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) (13, 14), although certain MHV strains also recognize heparan sulfate (15, 16). Some coronaviruses from each of the three genera recognize sugars (17-20). MERS-CoV belongs to the ␤-genus and uses human dipeptidyl peptidase 4 (DPP4) as its host receptor (21). Receptor recognition is a major determinant of coronavirus host range and tropism.An envelope-anchored trimeric spike p...

show abstract

“…MERS-CoV S protein 377-662 rezidülerindeki reseptör bağ-lanma/kritik nötralizan bölgenin tanımlanması bu yeni enfeksiyon için aşı geliştirilmesinde etkili olabilir (27)(28)(29).…”

Section: Tedavi̇unclassified

Ortadoğu Solunum Yetmezliği Sendromu Koronavirüsü

Nemli¹,

Demırdal²

2016

Kocatepe Tıp Dergisi

View full text Add to dashboard Cite

ÖZCoronavirüsler zarflı, tek zincirli pozitif RNA virüsleri olup, genellikle üst solunum yolu enfeksiyonlarına sebep olurlar. 2012 yılıda Arap Yarımadası'nda ciddi akut solunum yetmezliği ile seyreden hastalarda yeni bir coronavirüs türü izole edilmiştir. Middle East Respiratory Syndrome-coronavirus (MERS-CoV) olarak adlandırıl-mıştır. Asemptomatik hastalıktan, ağır pnömoni, çoklu organ yetmezliği ve ölüme kadar ilerleyebilen çeşitli klinik şekillere sebep olabilir. Patojene özgül bir tedavi bulunmamaktadır. MERS-CoV ile enfekte olan hastalarda enfeksiyon kontrol önlemlerinin uygulanması virüsün yayılımının önlenmesi açısından önemli bir yer tutar. Anahtar Kelimeler: Koronavirus, MERS, enfeksiyon ABSTRACTCoronaviruses are enveloped, positive-strand RNA viruses and usually cause upper respiratory tract infection in humans. In 2012, a novel coronavirus was found to cause cases of severe acute respiratory infection in Arabian Peninsula. This novel virüs has been defined as Middle East Respiratory Syndrome-coronavirus (MERS-CoV). Clinical presentation of MERS-CoV infection ranges from asymptomatic to severe pneumonia, multi-organ failure resulting in death. There's no pathogen specific antiviral therapy for MERS-CoV. Therapy strategy is based on supportive approach and prevention of complications. It is very important to implement infection prevention and control procedures to decrease the the risk of spread of virus.

show abstract

The Receptor Binding Domain of the New Middle East Respiratory Syndrome Coronavirus Maps to a 231-Residue Region in the Spike Protein That Efficiently Elicits Neutralizing Antibodies

Cited by 221 publications

References 25 publications

Middle East Respiratory Syndrome Coronavirus Spike Protein Delivered by Modified Vaccinia Virus Ankara Efficiently Induces Virus-Neutralizing Antibodies

Middle East Respiratory Syndrome Coronavirus Spike Protein Delivered by Modified Vaccinia Virus Ankara Efficiently Induces Virus-Neutralizing Antibodies

Crystal Structure of the Receptor-Binding Domain from Newly Emerged Middle East Respiratory Syndrome Coronavirus

Ortadoğu Solunum Yetmezliği Sendromu Koronavirüsü

Contact Info

Product

Resources

About