The recombinant rabbit hemorrhagic disease virus VP60 protein obtained from Pichia pastoris induces a strong humoral and cell-mediated immune response following intranasal immunization in mice

Farnós, Omar; Rodrı́guez, Manuel; Chiong, Maylin; Parra, Francisco; Boué, Oscar; Lorenzo, N. Alcorta; Colás, Manuel; Lleonart, Ricardo

doi:10.1016/j.vetmic.2005.11.062

Cited by 13 publications

(6 citation statements)

References 45 publications

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“…In addition, this would need to be performed systematically since induced-immunity lasts no longer than 1 year [222] and efficacy has been shown to be dependent on several physiological parameters of the individuals [223]. Therefore, alternative approaches are being explored to overcome these limitations such as the development of vaccines with the capacity for horizontal transmission to ensure appropriate immunization of a relevant portion of the population [219], vaccines that may be administered by the oral or nasal routes [105,208,213,217,219,224,225] or the construction of bivalent vaccines [216,226]. Nevertheless, as yet, none of these vaccines has been registered or is commercially available.…”

Section: Prevention Control and Vaccinationmentioning

confidence: 99%

Rabbit haemorrhagic disease (RHD) and rabbit haemorrhagic disease virus (RHDV): a review

Abrantes

Loo

Pendu

et al. 2012

Vet Res

344

452

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Rabbit haemorrhagic disease virus (RHDV) is a calicivirus of the genus Lagovirus that causes rabbit haemorrhagic disease (RHD) in adult European rabbits (Oryctolagus cuniculus). First described in China in 1984, the virus rapidly spread worldwide and is nowadays considered as endemic in several countries. In Australia and New Zealand where rabbits are pests, RHDV was purposely introduced for rabbit biocontrol. Factors that may have precipitated RHD emergence remain unclear, but non-pathogenic strains seem to pre-date the appearance of the pathogenic strains suggesting a key role for the comprehension of the virus origins. All pathogenic strains are classified within one single serotype, but two subtypes are recognised, RHDV and RHDVa. RHD causes high mortality in both domestic and wild adult animals, with individuals succumbing between 48-72 h post-infection. No other species has been reported to be fatally susceptible to RHD. The disease is characterised by acute necrotising hepatitis, but haemorrhages may also be found in other organs, in particular the lungs, heart, and kidneys due to disseminated intravascular coagulation. Resistance to the disease might be explained in part by genetically determined absence or weak expression of attachment factors, but humoral immunity is also important. Disease control in rabbitries relies mainly on vaccination and biosecurity measures. Such measures are difficult to be implemented in wild populations. More recent research has indicated that RHDV might be used as a molecular tool for therapeutic applications. Although the study of RHDV and RHD has been hampered by the lack of an appropriate cell culture system for the virus, several aspects of the replication, epizootology, epidemiology and evolution have been disclosed. This review provides a broad coverage and description of the current knowledge on the disease and the virus.

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Section: Prevention Control and Vaccinationmentioning

confidence: 99%

Rabbit haemorrhagic disease (RHD) and rabbit haemorrhagic disease virus (RHDV): a review

Abrantes

Loo

Pendu

et al. 2012

Vet Res

344

452

View full text Add to dashboard Cite

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“…The virion contains a 7.4Kb single-stranded positive-sense RNA genome. The capsid consists of a major protein component of 60 kDa (Vp60) [3] . Myxoma virus (MV), whose genome composes of 163kb nucleotides, belongs to the Leporipoxvirus genus of the Poxviridae family.…”

Section: Introductionmentioning

confidence: 99%

Notice of Retraction: Construction of Recombinant Rabbit Hemorrhagic Disease Virus (RHDV) Vaccine Using Myxoma Virus (MV) as a Vector

Guo

Wang

et al. 2011

2011 5th International Conference on Bioinformatics and Biomedical Engineering

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Rabbit hemorrhagic disease virus (RHDV) and myxoma virus (MV) are the two main viruses that cause serious virus diseases in rabbit population. MV, a pox virus, has been proved to be a good recombinant vaccine vector. In this study we have developed a recombinant virus using MV as a vector against both the myxomatosis and rabbit hemorrhagic disease (RHD). We used the nonessential gene MST3N of MV as the insertion site. The recombinant viruses expressed the RHDV major capsid protein (VP60) and the selectable marker GFP. The pure recombinant viruses were achieved after several rounds of plaque screening. Replication of MST3N-knockout recombinant virus MV-VP60 in Rabbit kidney cell (RK13) was unimpaired, compared with wild type virus MV. Keywords-Rabbit hemorrhagic disease virus (RHDV), myxoma virus (MV), non essential MST3N gene, vector.

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“…The prepared RHDV vaccines may be given for animals either orally (Bertagnoli et al, 1996a;Pla-na-Duran et al, 1996;Martin-Alonso et al, 2003;Farnos et al, 2005) or intra-nasal (Farnos et al, 2006). Moreover, bivalent vaccines against myxomatosis (Bertagnoli et al, 1996b;Barcena et al, 2000) and pasteurellosis (Peshev and Christova, 2003) have been also used.…”

Section: Prevention and Controlmentioning

confidence: 99%

A Review on Rabbit Hemorrhagic Disease with a Special Reference to Egyptian Situation

El-Ghany

2020

J Hellenic Vet Med Soc

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Rabbit hemorrhagic disease (RHD) is considered as one of the most important viral diseases that affects and threatens rabbit’s flocks. This disease has affected rabbits since mid-1980. Two epidemics of RHD had been discovered; the first was in mid-1980 and known as classical RHD virus (RHDV), while the second was in 2011 and described as variant virus (RHDVb/RHDV2). Domestic and wild rabbits are susceptible to RHD. All ages can be affected, but adults are more susceptible to young kitten. RHD is presented in three forms; per-acute, acute and subacute or chronic form. Mortality rate is usually high especially in per-acute and acute stages and it is associated with disseminated intravascular coagulopathy and necrotic hepatitis. The main lesions have been observed in the liver, lungs and spleen. Diagnosis of RHD is based on the clinical picture and detection of RHDV or specific antibodies. The prevention and control strategies depend mainly on using of preventive inactivated vaccine together with adoptionof hygienic measures. However, there is no specific treatment of RHDV infection. So, this review article puts a spot light on RHD regarding the epidemiology, the clinical and laboratory diagnosis as well as the prevention and control strategies with a special reference to Egyptian situation.

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The recombinant rabbit hemorrhagic disease virus VP60 protein obtained from Pichia pastoris induces a strong humoral and cell-mediated immune response following intranasal immunization in mice

Cited by 13 publications

References 45 publications

Rabbit haemorrhagic disease (RHD) and rabbit haemorrhagic disease virus (RHDV): a review

Rabbit haemorrhagic disease (RHD) and rabbit haemorrhagic disease virus (RHDV): a review

Notice of Retraction: Construction of Recombinant Rabbit Hemorrhagic Disease Virus (RHDV) Vaccine Using Myxoma Virus (MV) as a Vector

A Review on Rabbit Hemorrhagic Disease with a Special Reference to Egyptian Situation

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