The recombination mediated by double-strand breaks in extrachromosomal DNA substrate carrying mouse immunoglobulin switch regions Sμ and Sγ2b

Kameyama, Kohzoh; Sakaguchi, Gaku; Haga, Hisanori; Shibata, Naoko; Uryu, Masahide; Sugimoto, Kazunori

doi:10.1016/0167-4781(96)00124-8

Cited by 2 publications

(2 citation statements)

References 30 publications

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“…S-sequence-mediated recombination was recently documented in fibroblast cell lines on extrachromosomally replicating substrates (45). This finding might have been interpreted in light of the different fate of episomal and integrated substrates.…”

Section: Discussionmentioning

confidence: 96%

Sensitive analysis of recombination activity using integrated cell surface reporter substrates

Christine

Siebenkotten²,

Radbruch

1999

Cytometry

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Section: Discussionmentioning

confidence: 96%

Sensitive analysis of recombination activity using integrated cell surface reporter substrates

Christine

Siebenkotten²,

Radbruch

1999

Cytometry

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“…This recombination occurs between regions of repetitive DNA sequences, switch regions (S), which are located 5Ј of each heavy chain constant region gene, except ␦. The Ab isotype is not utilized randomly and specific stimuli induce B cell responses with characteristic isotype profiles (7)(8)(9)(10)(11).…”

mentioning

confidence: 99%

Regulation of Germline Promoters by the Two Human Ig Heavy Chain 3′ α Enhancers

Pan

Pardali

et al. 2000

The Journal of Immunology

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The human IgH 3′ enhancers, located downstream of each of the two Cα genes, modulate germline (GL) transcription of the IgH genes by influencing the activity of promoter-enhancer complexes upstream of the switch and intervening (I) regions. The regulation of GL α1 and α2 promoters by different human 3′ enhancer fragments was investigated in cell lines representing various developmental stages. Both α1HS1,2 and α2HS1,2 fragments show equally strong enhancer activity on the GL α1 and α2 promoters in both orientations when transiently transfected into a number of mature B cell line (DG75, CL-01, and HS Sultan). However, there is no activity in a human pre-B cell line (NALM-6) nor a human T cell line (Jurkat). HS3 shows no enhancer activity by itself in any of the cell lines, whereas a modest effect is noted using HS4 in the three mature B cell lines. However, the combination of the α2HS3-HS1,2-HS4 fragments, which together form a potential locus control region, displays a markedly stronger enhancer activity than the individual fragments with a differential effect on the α1 and α2 promoters as compared with the γ3 promoter. Our results suggest that the human GL α promoter may be regulated by two independent pathways. One pathway is induced by TGF-β1 which directs IgA isotype switch through activation of the GL α promoter and no TGF-β1-responsive elements are present in the different 3′ enhancer fragments. The other route is through the human 3′ enhancer regions that cis-up-regulate the GL α promoter activity in mature B cells.

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The recombination mediated by double-strand breaks in extrachromosomal DNA substrate carrying mouse immunoglobulin switch regions Sμ and Sγ2b

Cited by 2 publications

References 30 publications

Sensitive analysis of recombination activity using integrated cell surface reporter substrates

Sensitive analysis of recombination activity using integrated cell surface reporter substrates

Regulation of Germline Promoters by the Two Human Ig Heavy Chain 3′ α Enhancers

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