The rectal mucosa-associated microflora in patients with ulcerative colitis

Hartley, M. Gillian; Hudson, Michael J.; Swarbrick, E T; Hill, Michael J.; Gent, A. E.; Hellier, M. D.; Grace, R H

doi:10.1099/00222615-36-2-96

Cited by 76 publications

(41 citation statements)

References 34 publications

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“…Several lines of evidence support the hypothesis that intestinal bacteria play a role in the pathogenesis of adult IBD and pediatric IBD (6,12,18,32,36,37,42). Increased numbers of mucosa-associated Escherichia coli isolates were observed in both CD and UC (17,25).…”

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confidence: 68%

Microevolution in fimH Gene of Mucosa-Associated Escherichia coli Strains Isolated from Pediatric Patients with Inflammatory Bowel Disease

et al. 2012

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ABSTRACTSeveral studies reported increased numbers of mucosa-associatedEscherichia colistrains in patients with inflammatory bowel disease (IBD), encompassing Crohn's disease (CD) and ulcerative colitis (UC). The majority ofE. colistrains possess type 1 fimbriae, whose tip fibrillum protein, FimH, naturally undergoes amino acid replacements, an important process in the adaptation of commensalE. colistrains to environmental changes, like those observed in IBD and urinary tract infections. In this study, we analyzed mutational patterns in thefimHgene of 52 mucosa-associatedE. colistrains isolated from IBD and non-IBD pediatric patients, in order to investigate microevolution of this genetic trait. FimH-positive strains were also phylogenetically typed and tested for their adhesive ability on Caco-2 cells. Specific FimH alleles for each grouping feature were found. Mutations G66S and V27A were related to CD, while mutations A242V, V163A, and T74I were attributed to UC. Otherwise, the G66S, N70S, and S78N mutations were specifically attributed to B2/D phylogroups. The N70S and A119V mutations were related to adhesiveE. colistrains. Phylogroup B2, adhesive, and IBDE. colistrains showed a higher site substitution rate (SSR) in thefimHgene, together with a higher number of mutations. The degree of naïve mucosal inflammation was related to specific FimH alleles. Moreover, we could suggest that the V27A mutation is pathoadaptive for the CD intestinal habitat, while we could also suggest that both the N70S and S78N mutations are related to the more virulentE. coliB2 phylogroup. In conclusion, we found some FimH variants that seem to be more involved than others in the evolution of IBD pathogenesis.

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confidence: 68%

Microevolution in fimH Gene of Mucosa-Associated Escherichia coli Strains Isolated from Pediatric Patients with Inflammatory Bowel Disease

et al. 2012

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“…Another explanation could be the detection limit of these bacteria in the biopsy specimens. Since we are focusing on a subpopulation in a community which contains approximately 10 6 bacteria, a small difference in the number of organisms of a certain species might have a large impact on its detection. Remarkably, one amplicon with the highest sequence similarity (99%) to L. gasseri was found in descending colon biopsy samples of 9 of the 10 individuals.…”

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confidence: 99%

“…Most of these analyses with contents from sudden-death victims (15) or with biopsy samples from living individuals involved a culturing approach and focused on the attachment of certain probiotic strains (1,2,12), the presence of sulfate reducers (5,27), and/or on bacterial population levels in diseased persons (6). Since biopsy samples are very small in size and therefore more easily exposed to oxygen during sampling, the number of viable strict anaerobes might be reduced easily.…”

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confidence: 99%

Mucosa-Associated Bacteria in the Human Gastrointestinal Tract Are Uniformly Distributed along the Colon and Differ from the Community Recovered from Feces

Zoetendal¹,

Vilpponen-Salmela²,

Ben-Amor³

et al. 2002

Appl Environ Microbiol

733

556

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The human gastrointestinal (GI) tract harbors a complex community of bacterial cells in the mucosa, lumen, and feces. Since most attention has been focused on bacteria present in feces, knowledge about the mucosaassociated bacterial communities in different parts of the colon is limited. In this study, the bacterial communities in feces and biopsy samples from the ascending, transverse, and descending colons of 10 individuals were analyzed by using a 16S rRNA approach. Flow cytometric analysis indicated that 10 5 to 10 6 bacteria were present in the biopsy samples. To visualize the diversity of the predominant and the Lactobacillus group community, denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons was performed. DGGE analysis and similarity index comparisons demonstrated that the predominant mucosaassociated bacterial community was host specific and uniformly distributed along the colon but significantly different from the fecal community (P < 0.01). The Lactobacillus group-specific profiles were less complex than the profiles reflecting the predominant community. For 6 of the 10 individuals the community of Lactobacilluslike bacteria in the biopsy samples was similar to that in the feces. Amplicons having 99% sequence similarity to the 16S ribosomal DNA of Lactobacillus gasseri were detected in the biopsy samples of nine individuals. No significant differences were observed between healthy and diseased individuals. The observed host-specific DGGE profiles of the mucosa-associated bacterial community in the colon support the hypothesis that host-related factors are involved in the determination of the GI tract microbial community.

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“…Fecal levels of lactobacilli and bifidobacteria have been shown to be reduced in patients with inflammatory bowel diseases (IBD) such as Crohn's disease (CD) and ulcerative colitis (UC) (6,8,12) as well as in allergy (16), thus suggesting a role for these bacteria in the prevention of these diseases. For a number of gastrointestinal diseases, specific LAB strains have been used successfully.…”

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confidence: 99%

Disease-Dependent Adhesion of Lactic Acid Bacteria to the Human Intestinal Mucosa

Ouwehand

Salminen

Roberts

et al. 2003

Clin Vaccine Immunol

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Their adhesion to the intestinal mucosa is considered one of the main reasons for the beneficial health effects of specific lactic acid bacteria (LAB). However, the influence of disease on the mucosal adhesion is largely unknown. Adhesion of selected LAB to resected colonic tissue and mucus was determined in patients with three major intestinal diseases (i.e., diverticulitis, rectal carcinoma, and inflammatory bowel disease) and compared to healthy control tissue. All strains were observed to adhere better to immobilized mucus than to whole intestinal tissue. Two strains (Lactobacillus rhamnosus strain GG and L. reuteri) were found to exhibit diseasespecific adhesion to intestinal tissue. All tested strains, with the exception of L. rhamnosus strain GG, displayed disease-specific adhesion to intestinal mucus. These results suggest that strains with optimal binding characteristics for a particular intestinal disease can be selected.

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The rectal mucosa-associated microflora in patients with ulcerative colitis

Cited by 76 publications

References 34 publications

Microevolution in fimH Gene of Mucosa-Associated Escherichia coli Strains Isolated from Pediatric Patients with Inflammatory Bowel Disease

Microevolution in fimH Gene of Mucosa-Associated Escherichia coli Strains Isolated from Pediatric Patients with Inflammatory Bowel Disease

Mucosa-Associated Bacteria in the Human Gastrointestinal Tract Are Uniformly Distributed along the Colon and Differ from the Community Recovered from Feces

Disease-Dependent Adhesion of Lactic Acid Bacteria to the Human Intestinal Mucosa

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