The redox state of the ascorbate-dehydroascorbate pair as a specific sensor of cell division in tobacco BY-2 cells

Pinto, Maria Concetta de; Francis, Dennis; Gara, Laura De

doi:10.1007/bf01415704

Cited by 218 publications

(124 citation statements)

References 31 publications

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“…Similarly to our study, one of the antioxidative enzyme-L-ascorbate peroxidase 1 (spot no 9) was accumulated after ME-inducing treatment in maize and rapeseed (Joosen et al 2007;Cordewener et al 2009;Uváčková et al 2012). Its role can be more complex as in various plant cell culture systems high ascorbate levels stimulate cell division (de Pinto et al 1999).…”

Section: Discussionsupporting

confidence: 80%

Identification of proteins related to microspore embryogenesis responsiveness in anther cultures of winter triticale (×Triticosecale Wittm.)

et al. 2017

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For a better understanding of the physiological background of microspore embryogenesis (ME), the protein profile was analyzed in four winter triticale DH lines, which show extremely different embryogenic potential. The analysis were conducted with anthers at the phase of development optimal for ME induction and then after low temperature (LT, 3 weeks at 4°C) MEinducing tillers treatment. The sub-proteome of anthers was mapped by two-dimensional gel electrophoresis (2-DE). The protein species significantly more abundant (at least 2-fold) in responsive DH lines after LT treatment were chosen for identification by MALDI-TOF/TOF analysis. In total, 31 protein species were successfully identified as involved in the determination of microspore competence, stress response and in the regulation of ME induction. Microspore competence required sufficient energy supply and efficient system of cell protection that determine survival under prolonged LT stress treatment. LT stress was associated with increased accumulation of proteins typical for cell defence against oxidative stress (e.g., L-ascorbate peroxidase), chaperons (e.g., HSP70) and other enzymes/factors ensuring protein biosynthesis, stability and active cell divisions. Also here, effective cell defence required undisturbed energy supply. Among proteins that accumulated differentially in accordance with microspore embryogenic potential again the most important role seems to be played by the enzymes ensuring energy production and determining ability of plant stress adaptation. Two protein species (enolase, 12S storage protein), proposed earlier as candidates for markers of embryogenesis in other in vitro plant culture systems confirmed their utility for triticale anther cultures.

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Section: Discussionsupporting

confidence: 80%

Identification of proteins related to microspore embryogenesis responsiveness in anther cultures of winter triticale (×Triticosecale Wittm.)

et al. 2017

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“…The material was ground in liquid nitrogen and homogenized with 1 mL of cold 5% meta-phosphoric acid at 4°C in a porcelain mortar. The homogenate was centrifuged at 20,000 g for 15 min at 4°C, and the supernatant was collected for analysis of glutathione and ascorbate (or its analogues) according to de Pinto et al (1999).…”

Section: Extraction and Analysis Of Ascorbate And Glutathionementioning

confidence: 99%

Gigaspora margarita with and without its endobacterium shows adaptive responses to oxidative stress

et al. 2017

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Arbuscular mycorrhizal (AM) fungi experience oxidative stress during the plant-fungal interaction, due to endogenous reactive oxygen species (ROS) produced by fungal metabolism and exogenous ROS produced by plant cells. Here, we examine the responses to H2O2 in Gigaspora margarita, an AM fungus containing the endobacterial symbiont Candidatus Glomeribacter gigasporarum (CaGg). Previous studies revealed that G. margarita with its endobacterium produces more ATP and has higher respiratory activity compared to a cured line that lacks the endobacterium. This higher bioenergetic potential leads to higher production of ROS, and to a higher ROS-detoxifying capacity, suggesting a direct or indirect role of the endobacterium in modulating fungal anti-oxidant responses. To test the hypothesis that the fungal-endobacterial symbiosis may enhance the fitness of the AM fungus in the presence of oxidative stress, we treated the fungus with a sublethal concentration of H2O2 and performed RNA-seq analysis. Our results demonstrate that: i) irrespective of the endobacterium presence, G. margarita faces oxidative stress by activating multiple metabolic processes (methionine oxidation, sulfur uptake, the pentose phosphate pathway, activation of ROS-scavenger genes), ii) in the presence of its endobacterium, G. margarita upregulates some metabolic pathways, like chromatin status modifications and iron metabolism; iii) contrary to our hypothesis, the cured line responds to H2O2 by activating the transcription of specific ROS scavengers. We confirmed the RNA-seq findings by measuring the glutathione and ascorbate content, which was the same in both lines after H2O2 treatment. We conclude that both fungal lines

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“…The AsA and DHA contents were determined according to Kampfenkel et al (1995) with modifications by de Pinto et al (1999).…”

Section: Determination Of CD and Ni Contentsmentioning

confidence: 99%

Metal uptake, antioxidant status and membrane potential in maize roots exposed to cadmium and nickel

et al. 2014

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Root growth of the seedlings of maize cultivars Premia and Blitz exposed to 2 µM cadmium (Cd), nickel (Ni) or both metals acting simultaneously (Cd + Ni) for 72 h was significantly reduced but not ceased. The effect was more pronounced in the seedlings of the cv. Blitz. The heavy metals (HMs) contents increased significantly in the roots. Simultaneous application of metals had an antagonistic effect on either Cd or Ni uptake in Premia but not in Blitz. In control roots the contents of ascorbic acid (AsA) and dehydroascorbic acid (DHA) were lower and gluthatione (GSH) content was higher in Premia than in Blitz. A decrease of AsA content was induced by all metal treatments in Premia but only by Cd + Ni in Blitz while an increase was induced by single metals in this cultivar. All metal treatments increased DHA contents in both cultivars. GSH content decreased significantly in Premia treated with Cd or Cd + Ni, and in Blitz treated with Ni. Unlike the contents of AsA, DHA and GSH, the increased metal concentrations in root cells did not affect the membrane potential (EM). The changes in antioxidant contents depended on both, maize genotypes and HMs treatments. Nevertheless, the results indicated a role of antioxidative system in minimizing the effects of oxidative stress and protecting cell membranes in both maize cultivars.

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The redox state of the ascorbate-dehydroascorbate pair as a specific sensor of cell division in tobacco BY-2 cells

Cited by 218 publications

References 31 publications

Identification of proteins related to microspore embryogenesis responsiveness in anther cultures of winter triticale (×Triticosecale Wittm.)

Identification of proteins related to microspore embryogenesis responsiveness in anther cultures of winter triticale (×Triticosecale Wittm.)

Gigaspora margarita with and without its endobacterium shows adaptive responses to oxidative stress

Metal uptake, antioxidant status and membrane potential in maize roots exposed to cadmium and nickel

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