The Reduction of Triphenyltetrazolium Chloride by Dehydrogenases of Corn Embryos

“…Fresh material was treated with 2,3,5-triphenyl-tetrazolium chloride (TTC) 0.5 % for 24 hours, to allow its reduction by dehydrogenases if available, then sectioned and examined under the microscope for appearance of the red precipitate (Lakon, 1942;Roberts, 1950;Jensen, Sacks and Baldauski, 1951;Parker, 1951Parker, , 1952Parker, , 1953Fahn and Arnon, 1962).…”

Section: Introductionmentioning

confidence: 99%

Wood Fibres With Living Protoplasts

Fahn

Leshem

1963

New Phytologist

View full text Add to dashboard Cite

Summary It has been observed that in many woody plants the libriform fibres, and in some the fibre tracheids, retain living protoplasts in the entire sapwood. It is suggested, therefore, that the definition of fibres usually given in botanical textbooks, should be changed. From the preliminary study it appears that living protoplasts persist mainly in shrubs and subshrubs. As these life‐forms generally represent the woody vegetation of unfavourable habitats, it may be possible that living fibres are adaptive characters.

show abstract

“…According to some authors (Chaomei & Yanlin 1993;Volin et al 2000;Dubinov et al 2000;Henselová et al 2011) the plasma induces not only changes on the seed surface but also in some biochemical parameters. The quality and vigour of seeds are very often based on the estimation of the viability of seeds with the tetrazolium test using dehydrogenase systems (Jensen et al 1951;Smith 1952). Generally, the higher DHO activity was determined in embryo, than in root, what might depend on the respiratory or enzymatic activity at an early stage of the germination process.…”

Section: Growth Parametersmentioning

confidence: 99%

Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

et al. 2012

View full text Add to dashboard Cite

Abstract:The seeds of Zea mays L. cv. KWS were exposed to low-temperature plasma (LTP) by using Diffuse Coplanar Surface Barrier Discharge (DCSBD) for 60 and 120 seconds respectively. Growth parameters, anatomy of roots and activity of some enzymes (CAT, G-POX, SOD and DHO) isolated from roots grown from the seeds treated by LTP were evaluated. Our results indicate that LTP treatment of maize seeds affects post-germination growth of seedlings and this effect depends on the duration of LTP treatment. LTP treatment in duration of 60 seconds significantly increased the length, fresh and dry weight of the roots. However, the increase in time of LTP treatment to 120 seconds had inhibitive effect on the studied growth parameters. The activities of all studied antioxidant enzymes significantly increased with the age of maize seedlings in control conditions. On the other hand the application of LTP resulted in small, mostly non significant changes in the activity of antioxidant enzymes. Significant decrease in CAT activity was observed both in 3 and 6-day old maize roots and G-POX activity in 3-day old maize roots grown from seeds exposed to LTP for 60 seconds. A small, significant increase was detected only in SOD activity in 3-day old maize roots grown from seeds treated with LTP for 120 seconds and in 6-day old maize roots treated with LTP for 60 seconds. Significantly higher DHO activity was determined in embryos isolated from seeds treated with LTP for 60 seconds. On the contrary, in roots the DHO activity decreased with the time of LTP treatment. LTP treatment of seeds did not affect the anatomy of maize roots and caused only minor changes in the isoenzyme composition of G-POX and SOD.

show abstract

The Reduction of Triphenyltetrazolium Chloride by Dehydrogenases of Corn Embryos

Cited by 44 publications

References 8 publications

Biological Oxidations

Biological Oxidations

Wood Fibres With Living Protoplasts

Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

Contact Info

Product

Resources

About