The Reed-Sternberg cell/lymphocyte rosette. I. Properties of rosettes formed between Hodgkin's cell lines and allogeneic lymphocytes

Abstract

Summary The properties of rosettes formed between the Hodgkin's cell lines, L428 and L591, and allogeneic peripheral blood mononuclear cell populations have been investigated. Immunocytochemical analysis showed that the majority of adherent cells were T-cells of both the CD4 and CD8 subsets. Only relatively few B-cells and monocytes were seen to adhere. However, when peripheral blood mononuclear cell populations were fractionated, it was found that monocytes were as good as T-cells at forming rosettes with both L428 and L591, though B-cells were shown to be poor at forming such associations. Treatment of both L428 and L591 with neuraminidase resulted in a significant reduction (P <0.01) in the mean number of adherent lymphocytes and in the numbers of Hodgkin's tumour cells which formed rosettes. Smaller, less significant effects were observed for Cytochalasin B and trypsin. EDTA (10-2 M) at pH7.2 had no significant effect on rosetting for L428 or L591. Adherence of allogeneic lymphocytes to L428 or L591 was pH dependent but did not appear to correlate with cell surface charge. Treatment of L428 cells with Fab fragments prepared from the IgG fraction of a hyperimmune rabbit anti-L428 antiserum, significantly (P<0.05) inhibited the adherence of allogeneic lymphocytes, but only when used at high concentration. The binding requirements of the Hodgkin's cell lines with allogeneic peripheral blood lymphocytes, as described in this study, appear to be quite different from those described for freshly isolated Hodgkin's tumour cells with autologous intratumoral lymphocytes. This suggests that the two phenomena may be unrelated. There would appear to be an absolute requirement for cell surface sialic acid for allogeneic lymphocyte attachment to the HD cell lines. This might suggest that the receptor-ligand system involved contains sialic acid as an integral part of the cell surface receptor structure involved in recognition of the appropriate ligand.The adherence of T-lymphocytes to Hodgkin's mononuclear (HM) and Reed-Sternberg (RS) cells has been described both in fresh imprints of Hodgkin's disease tissue (Abdulaziz et al., 1984;Poppema et al., 1982) and for single cell suspensions prepared from involved lymph nodes and spleen (Sundeen et al., 1987;Stuart et al., 1977;Payne et al., 1980;Kadin et al., 1974). The significance of this association is unknown, although limited evidence for a host mediated cytotoxic attack on the tumour cell population has been claimed (Archibald & Frenster, 1973;Kay, 1976) but never substantiated. The survival of Hodgkin cell-lymphocyte rosettes for up to five weeks in vitro (Payne et al., 1977) and the observation that T-cells binding to the RS/HM cell are predominantly of the T helper (CD4+) subset (Morris & Stuart, 1984) strongly suggests that the interaction is not a cytotoxic attack on the tumour cell population. It has been further observed by Payne et al. (1980) that the binding of T-cells to the RS/HM cell is characteristically quite different from that observed for T-cell/target cel...

Lymphocyte predominant cells of nodular lymphocyte predominant Hodgkin lymphoma interact with rosetting T cells in an immunological synapse

“…Rosetting T cells in HL have been known for many years. 25,26 However, their nature was mostly enigmatic. Both the possibility of antigen presentation from Hodgkin-Reed-Sternberg cells to the T cells, 27,28 as well as a host-anti-lymphoma response have been discussed.…”

“…Rosetting T cells in HL have been known for many years 25,26 . However, their nature was mostly enigmatic.…”

Spindle-shaped CD163+ rosetting macrophages replace CD4+ T-cells in HIV-related classical Hodgkin lymphoma

“…We also found in cell culture experiments that monocytes are equally good at forming clusters and rosettes with Hodgkin cell lines, in line with a previous study. 19 Macrophage rosetting is, therefore, likely to occur in the absence of CD4 þ T-cells, but is not hampered by the presence of T-cells.…”

Rosetting T cells in Hodgkin lymphoma are activated by immunological synapse components HLA class II and CD58

“…This approach with unmatched PBMCs is consistent with most previous studies, but has the disadvantage that T-cell responses are largely attributable to alloreactivity. 6,11 We used HLA-II-matched PBMCs to more closely resemble the in vivo situation (supplemental Tables 1 and 2). HL cell lines were cocultured with PBMCs at a ratio of 1:20.…”