The regenerating liver: A site of erythropoiesis in the adult long‐evans rat

Naughton, Brian A.; Kolks, Gail A.; Arce, Janet M.; Liu, Philip L.‐F.; Gamba‐Vitalo, Christina; Piliero, Sam J.; Gordon, Albert S.

doi:10.1002/aja.1001560117

Cited by 19 publications

(14 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results also implicate previous reports that several dedifferentiated hepatoma cell lines express hematopoietic cytokines (23,24). Moreover, regenerating liver was shown to express hematopoietic cytokines (25,26). Thus, expression of hematopoietic cytokines in the liver appears closely related to the state of liver differentiation, and OSM may be involved in regulation of hematopoietic cytokines in the adult liver as well.…”

Section: Discussionsupporting

confidence: 91%

Hepatic differentiation induced by oncostatin M attenuates fetal liver hematopoiesis

Kinoshita

Sekiguchi

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

163

121

View full text Add to dashboard Cite

Embryonic liver is a transient site for definitive hematopoiesis. Along with maturation of the bone marrow and spleen, hematopoietic cells relocate from the liver to their final destinations while the liver starts organizing its own structure and develops numerous metabolic functions toward adult. Recently, it was demonstrated that the signal exerted by oncostatin M (OSM) through gp130 plays a pivotal role in the maturation process of the liver both in vitro and in vivo. However, the molecular basis underlying the termination of embryonic hematopoiesis remains unknown. In this study, we report that primary culture of fetal hepatic cells from embryonic day 14.5 murine embryos supported expansion of blood cells from Lin ؊ Sca-1 ؉ c-Kit ؉ cells, giving rise to myeloid, lymphoid, and erythroid lineages. Of interest, promotion of hepatic development by OSM and glucocorticoid strongly suppressed in vitro hematopoiesis. Consistent with these results, hepatic culture from the embryonic day 18.5 liver no longer supported hematopoiesis. These data together with the previous observations suggest that the signals exerted by OSM and glucocorticoid induce hepatic differentiation, which in turn terminate embryonic hematopoiesis and promote relocation of hematopoietic cells.

show abstract

Section: Discussionsupporting

confidence: 91%

Hepatic differentiation induced by oncostatin M attenuates fetal liver hematopoiesis

Kinoshita

Sekiguchi

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

163

121

View full text Add to dashboard Cite

show abstract

“…x 6,700 tion in the fetus. It is interesting that cases of liver hematopoiesis in the adult, as we have shown in the fetal rat, are sometimes characterized by nucleated red cells entering the peripheral circulation (Gunz, 1977;Naughton et al, 1979). Perhaps this indicates that the endothelial cell both in the fetal and the adult liver is incapable of differentiating between the maturational stages of the red cell with respect to diapedesis.…”

Section: Kupffer Cellsmentioning

confidence: 89%

The development of the sinusoids of fetal rat liver: Morphology of endothelial cells, Kupffer cells, and the transmural migration of blood cells into the sinusoids

Bankston

Pino

1980

Am. J. Anat.

View full text Add to dashboard Cite

The fine structural development of rat fetal liver sinusoids from 10 to 22 days gestation was studied. Colloidal carbon (Pelikan ink) was injected into 14-22 day gestation fetuses via the umbilical vein to assess the continuity of the sinusoidal lining and the phagocytic ability of the developing lining cells. Endothelial cells, devoid of an underlying basal lamina, form the bulk of the vascular lining at all gestational ages. These cells possess typical intercellular junctions and fenestrae with diaphragms before 17 days gestation. Transendothelial open fenestrations, typical of the adult liver, appear around 17 days gestation, increasing in number for the remainder of gestation. Although fenestrae possessing diaphragms are permeable to carbon before 16 days gestation, open fenestrations, first seen at 17 days gestation, allowed large amounts of carbon to reach the extravascular space. Endocytosis of carbon by endothelial cells was accomplished exclusively by large bristle-coated vesicles. Endothelial cells were also seen to be involved in transmural diapedesis of newly formed erythrocytes and megakaryocyte processes from the extravascular space by forming a temporary migration pore allowing these cells and processes to enter the circulation. At the end of gestation, blood-forming activity had nearly ceased, and only the space of Dissé separated the lining cells from the parenchymal cells. Kupffer cells were easily identified as early as 13 days gestation by their content of phagosomes and engulfed erythrocytes. The Kupffer cells are much more avid in the phagocytosis of carbon than are endothelial cells. Toward the end of gestation, some Kupffer cells develop a homogeneous "sticky coat" to carbon.

show abstract

“…We also reported that extramedullary hepatic hemaunder certain conditions. [1][2][3] In an in vivo experimental model topoiesis occurs only in close contact with LEC-1, suggesting of extramedullary hematopoiesis, we have reported the forthat these cells may provide the microenvironment necessary mation of hematopoietic colonies within the hepatic sinufor the maintenance and growth of hematopoietic cells. In the soids.…”

mentioning

confidence: 99%

Extramedullary hematopoiesis in the adult mouse liver is associated with specific hepatic sinusoidal endothelial cells

Cardier

Barberá‐Guillem

1997

Hepatology

View full text Add to dashboard Cite

The liver is an important hematopoietic organ during fetal In previous work, two anatomically distinct-liver sinusoid life. After birth, liver hematopoiesis ceases; however, the abilendothelial cells (LEC): LEC-1 and LEC-2, have been deity of the liver to support hematopoiesis can be reestablished scribed. We also reported that extramedullary hepatic hemaunder certain conditions. [1][2][3] In an in vivo experimental model topoiesis occurs only in close contact with LEC-1, suggesting of extramedullary hematopoiesis, we have reported the forthat these cells may provide the microenvironment necessary mation of hematopoietic colonies within the hepatic sinufor the maintenance and growth of hematopoietic cells. In the soids. 4,5 These results indicate that critical hematopoietic mipresent work, we studied the capacity of LEC-1 and LEC-2 croenvironment (HM) components are probably expressed to maintain in vitro hematopoiesis. LEC-1 and LEC-2 were within the liver sinusoids. We have also reported that the isolated and cloned from livers of adult mice. Bone marrow sinusoidal walls of the liver are composed of two kinds of cells (BM) enriched with primitive hematopoietic progenitors liver endothelial cells (LEC), type-1 and type-2 (LEC-1 and were isolated from day-2, post-5-FU-treated mice (5-FUBMC).LEC-2, respectively). 6,7 LEC-1 supported the maintenance and differentiation of he-The formation of hematopoietic foci during liver extramatopoietic progenitors for more than 6 weeks in vitro. In medullary hematopoiesis occurs only in the periportal docontrast, LEC-2 cells poorly supported the proliferation of mains of the liver, in close contact with LEC-1; 4,6,7 that locahematopoietic cells for only two weeks of the co-culture. LECtion suggests the possibility that these LECs may provide 1 and 5-FUBMC cocultures showed cobblestone-area formavarious factors necessary for the maintenance and growth of tion and the presence of hematopoietic progenitors that are the circulating hematopoietic stem cells. This evidence also able to form colonies (CFC) in the adhering fraction after six suggests that in vivo there is a differential capacity to support weeks of coculture. LEC-1 co-cultures treated with a cocktail hematopoiesis by LEC-1 and LEC-2 which are located in the of cytokines (stem cell factor, interleukin [IL] 1 a, IL-3, and hepatic sinusoids. The HM is a complex system composed, Epo) showed that megakaryocyte (CFU-Mk) and erythrocyte mainly, of stromal cells, such as endothelial cells, fibroblasts, progenitors (BFU-e) were present during the entire period of adipocytes, osteoclasts, and monocytes which secrete cytothe culture. Granulocyte-macrophage progenitors (CFU-GM) kines, produce extracellular matrix, and mediate direct celluwere present only during the first three weeks of the culture. lar contact that regulates in vivo and in vitro hematopoieThese results suggest that LEC-1, but not LEC-2, provide an sis. [8][9][10]

show abstract

The regenerating liver: A site of erythropoiesis in the adult long‐evans rat

Cited by 19 publications

References 13 publications

Hepatic differentiation induced by oncostatin M attenuates fetal liver hematopoiesis

Hepatic differentiation induced by oncostatin M attenuates fetal liver hematopoiesis

The development of the sinusoids of fetal rat liver: Morphology of endothelial cells, Kupffer cells, and the transmural migration of blood cells into the sinusoids

Extramedullary hematopoiesis in the adult mouse liver is associated with specific hepatic sinusoidal endothelial cells

Contact Info

Product

Resources

About