The Regional Distribution and Relative Frequency of Gastrointestinal Endocrine Cells in SHK‐1 Hairless Mice: An Immunohistochemical Study

Abstract

The regional distributions and relative frequencies of some gastrointestinal endocrine cells in the eight portions (fundus, pylorus, duodenum, jejunum, ileum, caecum, colon and rectum) of the gastrointestinal tract of SKH-1 hairless mice were investigated using immunohistochemical methods and seven types of specific antisera against somatostatin, serotonin, glucagon, cholecystokinin (CCK)-8, secretin, pancreatic polypeptide (PP) and gastrin. In this study, somatostatin-, serotonin-, glucagon-, CCK-8-, secretin- and gastrin-immunoreactive (IR) cells were identified. Most of these IR cells in the intestinal portion were generally spherical or spindle-shaped (open-type cell) while cells that were round in shape (close-type cell) were occasionally found in the stomach regions. Their relative frequencies were varied according to each portion of gastrointestinal tract. Somatostatin-IR cells were found throughout the gastrointestinal tract except for the large intestine. Serotonin-IR cells were detected throughout the whole gastrointestinal tract and were the most predominant endocrine cell types in this species of mouse. Glucagon-IR cells were restricted to the fundus, occurring rarely. CCK-8-IR cells were observed in the pylorus, duodenum and jejunum with frequencies that were numerous, moderate and few, respectively. Peculiarly, secretin-IR cells were demonstrated in the whole intestinal tract with either few or rare frequencies. Gastrin-IR cells were restricted to the pylorus and were numerous. However, no PP-IR cells were found in this study. In conclusion, some peculiar distributional patterns of gastrointestinal endocrine cells were found in SKH-1 hairless mouse.

Combined administration of secretin and oxytocin inhibits chronic colitis and associated activation of forebrain neurons

“…Secretin is produced in the duodenum by the ‘S’ subtype of enteroendocrine cell, but these cells are rare 58 or absent 57 in adult colon. Colonic S cells are more common during development.…”

An Immunohistochemical Study of Gastrointestinal Endocrine Cells in the BALB/c Mouse

“…In addition, these IR cells were detected in the whole alimentary tract including the oesophagus of low vertebrates (Ku et al., 2000). Serotonin‐IR cells were detected in the whole GIT of the gerbil (Lee et al., 2000), common tree shrew (Yamada et al., 1999), Philippine carabao (Baltazar et al., 1998), the Manchurian chipmunk (Lee et al., 1998), rat (Inokuchi et al., 1982) and mouse (Sandstrom and El‐Salhy, 2000; Ku et al., 2002). In the present study, serotonin‐IR cells were detected throughout the whole GIT and showed the highest frequencies in the fundus.…”

“…In addition, the changes of regional distribution and relative frequency of some gastrointestinal endocrine cells in mice with ageing were also reported (Sandstrom et al., 1998, 1999; Sandstrom and El‐Salhy, 2000). Ku et al. (2002) reported strain‐dependent differences in the distribution and frequency of gastrointestinal endocrine cells in the hairless mouse compared with other mouse strains.…”

“…Many studies have elucidated the regional distribution and relative frequency of different endocrine cells in the gastrointestinal tract (GIT) of various vertebrates including various species of rodents. In rodents, the distribution and relative frequency of endocrine cells in the GIT of the Manchurian chipmunk (Lee et al., 1997a, 1998), gerbil (Lee et al., 2000) and hairless mouse (Ku et al., 2002) were demonstrated, and the distribution of endocrine cells in the intestinal tract was also detected in the Korean tree squirrel (Lee et al., 1991, 1997b). In addition, Spangeus and El‐Salhy (1998) and Spangeus et al.…”

IMUNOLOCALIZAÇÃO DE ENTEROGLUCAGON EM CÉLULAS ENDÓCRINAS PRESENTES NO ESTÔMAGO DO MUÇUÃ <italic>Kinosternon scorpioides</italic> (REPTILIA, CHELONIA, KINOSTERNIDAE)

“…Células imunorreativas ao glucagon foram detectadas ao longo do estomago do G. japonicus, para o S. indicus foi encontrada atividade no íleo e no reto e para a espécie F. chinesis no íleo, no reto e raramente na fúndica (17) (Tabela 1). Ku et al (18) estudaram a distribuição regional e frequência relativa de células endócrinas no trato alimentar de Trachemys scripta elegans, por meio de métodos imuno-histoquímicos usando 10 anti-soros. Essas células imunorreativas estavam localizadas na porção basal do intestino, no esôfago e nas glândulas gástricas do estômago.…”