The regulation of antibiotic production in Streptomyces coelicolor A3(2)

Bibb, Mervyn J.

doi:10.1099/13500872-142-6-1335

Cited by 194 publications

(172 citation statements)

References 101 publications

(156 reference statements)

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“…11,21 We showed that scandium and lanthanum are both effective not only for enhancement of actinorhodin production but also for activation of silent or poorly expressed genes in S. coelicolor. In particular, scandium appears to be a feasible reagent for gene activation because it showed a rather wide range of effective concentrations.…”

Section: Resultsmentioning

confidence: 97%

Rare earth elements activate the secondary metabolite–biosynthetic gene clusters in Streptomyces coelicolor A3(2)

2010

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Genome sequencing projects have revealed many biosynthesis gene clusters for the production of as-yet unknown secondary metabolites, especially in actinomycetes. Here, we report that the rare earth elements, scandium and/or lanthanum, markedly activate, ranging from 2.5-to 12-fold, the expression of nine genes belonging to nine secondary metabolite-biosynthetic gene clusters of Streptomyces coelicolor A3(2) when added to the medium at low concentrations. HPLC analysis of ethyl acetateextractable metabolites indicated the detectability of several compounds only in the rare earth-treated cultures. This approach should facilitate discovery of new biologically active compounds and the study of secondary metabolite production.

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Section: Resultsmentioning

confidence: 97%

Rare earth elements activate the secondary metabolite–biosynthetic gene clusters in Streptomyces coelicolor A3(2)

2010

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“…Since actinorhodin is produced later in development than undecylprodigiosin (2), this phenotype may be the result of an arrest in development at a time where actinorhodin production has not yet been initiated.…”

Section: Discussionmentioning

confidence: 99%

ssgA Is Essential for Sporulation of Streptomyces coelicolor A3(2) and Affects Hyphal Development by Stimulating Septum Formation

et al. 2000

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The role of ssgA in cell division and development of streptomycetes was analyzed. An ssgA null mutant of Streptomyces coelicolor produced aerial hyphae but failed to sporulate, and ssgA can therefore be regarded as a novel whi gene. In addition to the morphological changes, antibiotic production was also disturbed, with strongly reduced actinorhodin production. These defects could be complemented by plasmid-borne ssgA. In the wild-type strain, transcription of ssgA was induced by nutritional shift-down and was shown to be linked to that of the upstream-located gene ssgR, which belongs to the family of iclR-type transcriptional regulator genes. Analysis of mycelium harvested from liquid-grown cultures by transmission electron microscopy showed that septum formation had strongly increased in ssgA-overexpressing strains in comparison to wild-type S. coelicolor and that spore-like compartments were produced at high frequency. Furthermore, the hyphae were significantly wider and contained irregular and often extremely thick septa. These data underline the important role for ssgA in Streptomyces cell division.

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“…A particular feature of this strain is its production of four antibiotics, all of different chemical classes, allowing analysis of the regulation of antibiotic biosynthesis at different levels. These include pathwayspecific regulation, pleiotropic control of more than one antibiotic, and co-regulation of antibiotic production and morphological development (Bibb, 1996;Chater & Bibb, 1997;.…”

Section: Introductionmentioning

confidence: 99%

Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2)

et al. 1998

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Putative peptide-synthetase-encoding DNA fragments were isolated from the Streptomyces coelicolor A3(2) chromosome using a PCR-based approach and mapped to a single -35 kb segment. In integrative transformation experiments, DNA fragments from this region disrupted production of the calcium-dependent antibiotic (CDA) and had sequences characteristic of nonribosomal peptide synthetases, thus proving that the cda locus had been cloned.

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The regulation of antibiotic production in Streptomyces coelicolor A3(2)

Abstract: The regulation of antibiotic production in Streptomyces coelicolor A3(2)

Cited by 194 publications

References 101 publications

Rare earth elements activate the secondary metabolite–biosynthetic gene clusters in Streptomyces coelicolor A3(2)

Rare earth elements activate the secondary metabolite–biosynthetic gene clusters in Streptomyces coelicolor A3(2)

ssgA Is Essential for Sporulation of Streptomyces coelicolor A3(2) and Affects Hyphal Development by Stimulating Septum Formation

Physical identification of a chromosomal locus encoding biosynthetic genes for the lipopeptide calcium-dependent antibiotic (CDA) of Streptomyces coelicolor A3(2)

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