The regulation of cytosolic epoxide hydrolase in mice

Pichare, M. M.; Gill, Sarjeet S.

doi:10.1016/0006-291x(85)91866-2

Cited by 15 publications

(2 citation statements)

References 18 publications

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“…This suggested that in EPXH2, the Sp1 site immediately upstream of the putative transcriptional start site is essential for expression. Because Sp1 expression is generally ubiquitous [64] such a promoter system could explain the ubiquitous expression of sEH in numerous tissues in mammals [17,20,21,23,[25][26][27][28][29][30][65][66][67]. However, it does not explain the regulation of EPHX2 by PPAR-alpha agonists and testosterone as predicted from in vivo induction studies in rodents.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional regulation of the human soluble epoxide hydrolase gene EPHX2

Tanaka

Kamita

Wolf

et al. 2008

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanism

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Soluble epoxide hydrolase (sEH) is a multifunctional protein encoded by the EPHX2 gene. The biological functions and enzyme kinetics of sEH have been extensively investigated, however, little is known about its transcriptional regulation and mechanisms of tissue specific expression. Here, a luciferase gene based reporter assay was used to identify the minimal promoter and cis regulatory elements of EPHX2. The minimal promoter was identified as a GC-rich region between nts −374 to +28 with respect to the putative transcriptional start site. A reporter plasmid carrying this minimal promoter showed higher or similar activities in comparison to a reporter plasmid carrying nts −5,974 to +28 of EPHX2 in 9 human cell lines that were tested. Sp1 binding sites that are involved in augmenting the minimal promoter activity of EPHX2 were identified by nested deletion analysis, site-specific mutation, electrophoretic mobility shift assay, and chromatin immunoprecipitation assay.

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Section: Discussionmentioning

confidence: 99%

Transcriptional regulation of the human soluble epoxide hydrolase gene EPHX2

Tanaka

Kamita

Wolf

et al. 2008

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanism

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“…Another reasonable consideration is that the two immunorelated forms of sEH are the products of two genes. Administration of the hypolipidemic drug clofibrate in the diets of rodents causes an increase in cytosolic sEH activity in liver cells, whereas peroxisomal sEH activity appears to be unaltered [33–36]. Hollinshead and Meijer [9], on the other hand, showed that clofibrate treatment did not lead to an increase in the cytosolic form of sEH.…”

Section: Discussionmentioning

confidence: 99%

Differential subcellular localization of endogenous and transfected soluble epoxide hydrolase in mammalian cells: evidence for isozyme variants

Mullen¹,

Trelease²,

Duerk³

et al. 1999

FEBS Letters

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Endogenous, constitutive soluble epoxide hydrolase in mice 3T3 cells was localized via immunofluorescence microscopy exclusively in peroxisomes, whereas transiently expressed mouse soluble epoxide hydrolase (from clofibrate-treated liver) accumulated only in the cytosol of 3T3 and HeLa cells. When the C-terminal Ile of mouse soluble epoxide hydrolase was mutated to generate a prototypic putative type 1 PTS (-SKI to -SKL), the enzyme targeted to peroxisomes. The possibility that soluble epoxide hydrolase-SKI was sorted slowly to peroxiosmes from the cytosol was examined by stably expressing rat soluble epoxide hydrolase-SKI appended to the green fluorescent protein. Green fluorescent protein soluble epoxide hydrolase-SKI was strictly cytosolic, indicating that -SKI was not a temporally inefficient putative type 1 PTS. Import of soluble epoxide hydrolase-SKI into peroxisomes in plant cells revealed that the context of -SKI on soluble epoxide hydrolase was targeting permissible. These results show that the C-terminal -SKI is a non-functional putative type 1 PTS on soluble epoxide hydrolase and suggest the existence of distinct cytosolic and peroxisomal targeting variants of soluble epoxide hydrolase in mouse and rat.z 1999 Federation of European Biochemical Societies.

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Immunoaffinity Purification and Comparison of Epoxide Hydrolases from Liver Cytosol and Peroxisomes of Untreated and Clofibrate-Treated Mice

Meijer

DePierre

1988

Archives of Toxicology

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The regulation of cytosolic epoxide hydrolase in mice

Cited by 15 publications

References 18 publications

Transcriptional regulation of the human soluble epoxide hydrolase gene EPHX2

Transcriptional regulation of the human soluble epoxide hydrolase gene EPHX2

Differential subcellular localization of endogenous and transfected soluble epoxide hydrolase in mammalian cells: evidence for isozyme variants

Immunoaffinity Purification and Comparison of Epoxide Hydrolases from Liver Cytosol and Peroxisomes of Untreated and Clofibrate-Treated Mice

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