The regulation of rabbit oviduct fluid formation

Abstract

The control of the formation of rabbit oviduct fluid and its relationship to the secretion of chloride ions has been studied using an in vitro vascularly perfused preparation. Fluid was produced at a rate of 43.41 microliters h-1 in oviducts from rabbits in oestrus. The rate was increased by isoprenaline and tetraethylammonium, decreased by dibutyryl cAMP, dihydro4,4'diisothiocyanatostilbene-2,2'-disulfonic acid (H2DIDS), and propranolol, while amiloride had no effect. H2DIDS induced a small decrease and isoprenaline a small increase in vascular to lumen Cl- flux but propranolol and dibutyryl cAMP had no effect. Oviducts from pseudopregnant animals treated with hCG three days before the experiment produced significantly less fluid than did those from rabbits in oestrus, but there was no difference in vascular to lumen Cl- flux. The concentration of K+ in oviduct fluid formed in vitro was more than three times higher than in the vascular perfusate. The ability of adrenergic agents to influence the formation of rabbit oviduct fluid could have clinical implications in the prevention or treatment of female infertility due to blockage of the Fallopian tubes and might also be useful in enhancing the secretory activity of oviduct cells maintained in co-culture with early embryos.

Physiology: Human tubal fluid: formation and composition during vascular perfusion of the Fallopian tube

“…The rate of fluid production was similar to that found in the rabbit oviduct (Dickens and Leese, 1994), and the concentrations of metabolites in the tubal fluid were close to those reported for other species (Holmdahl and Mastroianni, 1965;Leese, 1988), although the lactate concentration was a little higher than expected. This may have been due to lactate formation during the short anoxic interval which occurred while the tube was being cannulated.…”

“…These criteria are satisfied by the technique of vascular perfusion, which has been applied to the rabbit oviduct (Leese and Gray, 1985;Gott et al, 1988;Dickens and Leese, 1994). We now report the application of this method to the human Fallopian tube, together with an analysis of the tubal fluid formed for three nutrients, glucose, pyruvate and lactate, which are important in early human embryo metabolism .…”

Ovarian stimulation with human chorionic gonadotropin and equine chorionic gonadotropin affects prostacyclin and its receptor expression in the porcine oviduct

“…Many factors determine fluid secretion, like steroid hormones, VEGF, or PGs [37,38]. The increased capacity to synthesize PGI 2 , occurring on days 2 to 3 in mice [13] and porcine oviducts (present study), may indicate an important role of PGI 2 in tubal fluid production and release.…”

Expression of adrenergic receptors in mouse preimplantation embryos and ovulated oocytes

“…Epinephrine and norepinephrine present in oviductal fluid could influence the oviduct epithelium via adrenergic receptors which have been shown in oviduct epithelial cells of several species (Tolszczuk & Pelletier 1988, Dickens et al 1993, Einspanier et al 1999. Increased fluid formation, effects on Cl K ion transport and electrical potential differences have been shown after treatment of rabbit oviductal cells with agonists of adrenergic receptors (Dickens et al 1993, Dickens & Leese 1994. Besides, the effects on the oviduct epithelium, adrenoceptor agonists can also influence the functioning of spermatozoa in the oviduct, including capacitation and acrosome reaction (Way & Killian 2002, Adeoya-Osiguwa et al 2006.…”