The regulation of renal ammoniagenesis in the rat by extracellular factors. I. The combined effects of acidosis and physiologic fuels

Preuss, Harry G.; Eastman, Sarah T.; Vavatsi-Manos, O.; Baird, Kathleen L.; Roxe, David M.

doi:10.1016/0026-0495(78)90285-8

Cited by 19 publications

(5 citation statements)

References 30 publications

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“…L-glutamine is both a major ammoniagenic and ATP precursor for the renal proximal tubular cells. If an alternative metabolic substrate, such as free fatty acids, is provided to the proximal renal tubular cell, the result is a reduction in glutamine usage and ammoniagenesis [25][26][27][28][29][30]. It is of interest that insulin resistance is often associated with excessive plasma free fatty acid circulation [31].…”

Section: Discussionmentioning

confidence: 99%

The metabolic syndrome and uric acid nephrolithiasis: Novel features of renal manifestation of insulin resistance

Abate

Chandalia

Cabo-Chan

et al. 2004

Kidney International

488

307

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Section: Discussionmentioning

confidence: 99%

The metabolic syndrome and uric acid nephrolithiasis: Novel features of renal manifestation of insulin resistance

Abate

Chandalia

Cabo-Chan

et al. 2004

Kidney International

488

307

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“…(11) are is that lactate inhibits glutaminase activity, since Preuss et al 33 observed that lactate inhibited glutaminase activity in rat kidney slices.…”

Section: Ammonium Productionmentioning

confidence: 96%

Mathematical descriptions of hybridoma culture kinetics: I. Initial metabolic rates

Glacken

Adema

Sinskey

1988

Biotech & Bioengineering

182

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The lack of quantitative descriptions of mammalian culture kinetics limits the ability to optimally design and control cell culture bioreactors. This limitation is ad dressed by developing mathematical equations relating the initial growth rate and the antibody productivity of the hybridoma cell line, CRL-1606, to its environmental state. This initial rate approach, in contrast with steady state analysis of chemostat cultures, approximates steady state behavior, since the rates were measured over brief time intervals and at low cell concentrations (< 50,000 cells/mL). The advantage of this approach is that it is much faster than the chemostat approach.An equation for the growth rate was developed that superimposed Monod equations in serum and glutamine with "noncompetitive" -type inhibition constants were inversely proportional to the lactate and ammonium concentrations. The Monod constant is critical for relating initial, low cell level culture states to other states.Lactate was found to be the only environmental parameter that significantly inhibited antifibronectin monoclonal antibody production by the CRL-1606 hybridomas. Volumetric productivity was strictly related to culture viability, which was observed to decline at growth rates below 0.02 h(-1). Lactate was also found to significantly inhibit ammonium production.

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“…Flasks were kept on ice for an additional 15 min. Ammonia production over this incu bation time frame is fairly linear [18]. After the slices were removed, the excesses of perchlorate were precip itated by the addition of potassium hydroxide-phos phate buffer to make the final pH around 7.0.…”

Section: Methodsmentioning

confidence: 99%

Effects of 2-Oxoglutarate Concentrations on Canine Slice Ammoniagenesis

Risquez¹,

Gaydos²,

Preuss³

1983

Kidney Blood Press Res

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The concentration of renal 2-oxoglutarate has been proposed as an important regulator of ammoniagenesis in dog kidneys. In the present study, canine kidney slices produced less ammonia from glutamine and glutamate when 2-oxoglutarate was present in the incubation medium. However, the addition of arsenite, a metabolic blocker known to block 2-oxoglutarate metabolism and lead to its accumulation, overcame 2-oxoglutarate inhibition of ammoniagenesis when glutamine and glutamate were the ammonia precursors. Therefore, metabolism of 2-oxoglutarate, rather than its concentration, governed ammonia production from glutamine and glutamate in incubating dog renal tissue. In contrast to the results with 2-oxoglutarate, inhibition of glutamine ammoniagenesis by glutamate was not overcome by arsenite. The results suggest that renal ammonia adaptation in acidotic dogs cannot be ascribed to a theory based upon 2-oxoglutarate concentrations controlling the direction of the glutamate dehydrogenase pathway (GDH), decreasing glutamine transport, or directly inhibiting GDH enzyme activity.

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The regulation of renal ammoniagenesis in the rat by extracellular factors. I. The combined effects of acidosis and physiologic fuels

Cited by 19 publications

References 30 publications

The metabolic syndrome and uric acid nephrolithiasis: Novel features of renal manifestation of insulin resistance

The metabolic syndrome and uric acid nephrolithiasis: Novel features of renal manifestation of insulin resistance

Mathematical descriptions of hybridoma culture kinetics: I. Initial metabolic rates

Effects of 2-Oxoglutarate Concentrations on Canine Slice Ammoniagenesis

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