The regulation of the pentose phosphate pathway: Remember Krebs

Ramos-Martı́nez, Juan Ignacio

doi:10.1016/j.abb.2016.12.012

Cited by 58 publications

(30 citation statements)

References 18 publications

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“…To assess the potential of our methodological concept, we first selected five metabolic enzymes that catalyze key reactions of cellular primary carbohydrate metabolism, including glucose-6-phosphate dehydrogenase (G6PD; PPP), glyceraldehyde 3-phosphate dehydrogenase (GAPDH; glycolysis), lactate dehydrogenase (LDH; fermentation), and mitochondrial isocitrate dehydrogenase 3 (IDH3; TCA cycle) as well as succinate dehydrogenase (SDH; TCA cycle) ( Figure 1A). G6PD activity is a rate-limiting factor for glucose entering the oxidative PPP and therefore involved in nucleotide biosynthesis and in tuning cellular redox states (Ramos-Martinez, 2017;Stanton, 2012). GAPDH activity represents a central hub in glycolysis, can act as a rate-limiting step in aerobic glycolysis, and bridges meta-bolism and cell function (Chang et al, 2013;Colell et al, 2009;Schuppe-Koistinen et al, 1994;Shestov et al, 2014;Tristan et al, 2011).…”

Section: Enzyme Histochemistry Is a Valid Tool To Assess Enzymatic Acmentioning

confidence: 99%

Exploring Metabolic Configurations of Single Cells within Complex Tissue Microenvironments

et al. 2017

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Over the past years, plenty of evidence has emerged illustrating how metabolism supports many aspects of cellular function and how metabolic reprogramming can drive cell differentiation and fate. Here, we present a method to assess the metabolic configuration of single cells within their native tissue microenvironment via the visualization and quantification of multiple enzymatic activities measured at saturating substrate conditions combined with subsequent cell type identification. After careful validation of the approach and to demonstrate its potential, we assessed the intracellular metabolic configuration of different human immune cell populations in healthy and tumor colon tissue. Additionally, we analyzed the intercellular metabolic relationship between cancer cells and cancer-associated fibroblasts in a breast cancer tissue array. This study demonstrates that the determination of metabolic configurations in single cells could be a powerful complementary tool for every researcher interested to study metabolic networks in situ.

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Section: Enzyme Histochemistry Is a Valid Tool To Assess Enzymatic Acmentioning

confidence: 99%

Exploring Metabolic Configurations of Single Cells within Complex Tissue Microenvironments

et al. 2017

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“…The pentose phosphate pathway (PPP), also known as the phosphogluconate pathway or the hexose monophosphate shunt, is a metabolic pathway parallel to glycolysis, and represents the first committed step of glucose metabolism (6). The PPP serves a pivotal role in supporting cancer cell survival and growth by generating pentose phosphate for nucleic acid synthesis and providing nicotinamide-adenine dinucleotide phosphate (NADPH), which is needed for fatty acid synthesis and cell survival under stress conditions (7).…”

Section: Introductionmentioning

confidence: 99%

Crucial role of the pentose phosphate pathway in malignant tumors (Review)

2019

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Interest in cancer metabolism has increased in recent years. The pentose phosphate pathway (PPP) is a major glucose catabolism pathway that directs glucose flux to its oxidative branch and leads to the production of a reduced form of nicotinamide adenine dinucleotide phosphate and nucleic acid. The PPP serves a vital role in regulating cancer cell growth and involves many enzymes. The aim of the present review was to describe the recent discoveries associated with the deregulatory mechanisms of the PPP and glycolysis in malignant tumors, particularly in hepatocellular carcinoma, breast and lung cancer. Contents 1. Introduction 2. Glucose in the PPP 3. Glucose breakdown through glycolysis in the PPP 4. PPP in malignant tumors 5. Perspectives

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“…In particular, G6PD is a rate-limiting enzyme to regulate PPP flux and to switch glucose metabolism in hexose monophosphate shunt which goes in parallel to glycolysis pathway. [21] Taking into account that TIGAR can reprogram the glucose metabolism pathway from anaerobic oxidative phosphorylation to aerobic glycolysis, TIGAR could recognized as a key trigger of the balance normal-malignant metabolism. Enhanced expression of TIGAR can ameliorate autophagy impairment and the decrease in G6PD in the neurons exposed to high glucose.…”

Section: Discussionmentioning

confidence: 99%

The Effect of TIGAR Knockdown on Apoptotic and Epithelial‐Mesenchymal Markers Expression in Doxorubicin‐Resistant Non‐Small Cell Lung Cancer A549 Cell Lines

Ağca

Kırıcı

Nedzvetsky

et al. 2020

Chemistry & Biodiversity

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Resistance to chemotherapeutic drugs is a critical problem in cancer therapy, but the underlying mechanism has not been fully elucidated. TP53‐induced glycolysis regulatory phosphatase (TIGAR), an important glycolysis and apoptosis regulator, plays a crucial role in cancer cell survival by protecting cells against oxidative stress‐induced apoptosis. In the present study, we investigated whether TIGAR is involved in epithelial‐mesenchymal transition (EMT) in doxorubicin (DOX)‐resistant human non‐small cell lung cancer (NSCLC), A549/DOX cells. We found that the expression of TIGAR was significantly higher in A549/DOX cells than in the parent A549 cell lines. siRNA‐mediated TIGAR knockdown reduced migration, viability and colony survival of doxorubicin‐resistant lung cancer cells. Also, TIGAR knockdown decreased pro‐survival protein Bcl‐2 and increased pro‐apoptotic Bax and cleaved poly (ADP‐ribose) polymerase (PARP). Moreover, TIGAR depletion significantly up‐regulated both caspase‐3 and caspase‐9 expression. Furthermore, TIGAR depletion up‐regulated the expression of E‐cadherin and down‐regulated the expression of vimentin. These results indicate that TIGAR knockdown may inhibit EMT in doxorubicin (DOX)‐resistant human NSCLC and may represent a therapeutic target for a non‐small lung cancer cells chemoresistance.

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The regulation of the pentose phosphate pathway: Remember Krebs

Cited by 58 publications

References 18 publications

Exploring Metabolic Configurations of Single Cells within Complex Tissue Microenvironments

Exploring Metabolic Configurations of Single Cells within Complex Tissue Microenvironments

Crucial role of the pentose phosphate pathway in malignant tumors (Review)

The Effect of TIGAR Knockdown on Apoptotic and Epithelial‐Mesenchymal Markers Expression in Doxorubicin‐Resistant Non‐Small Cell Lung Cancer A549 Cell Lines

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