The regulation of TNF receptor mRNA synthesis, membrane expression, and release by PMA- and LPS-stimulated human monocytic THP-1 cells in vitro

Gatanaga, Tetsuya; Hwang, Chanseok; Gatanaga, Maki; Cappuccini, Fabio; Yamamoto, Robert S.; Granger, Gale A.

doi:10.1016/0008-8749(91)90127-w

Cited by 29 publications

(18 citation statements)

References 33 publications

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“…The plasma membrane levels of TNF-R1 were found to be significantly lower on diseased thyrocytes when compared to normal thyrocyte TNF-R1 surface levels. Soluble TNF-a receptor production has been shown to be accompanied by decreases in plasma membrane receptor levels (10,32,35). Therefore, soluble receptor production by diseased and normal thyrocytes was examined.…”

Section: Discussionmentioning

confidence: 99%

“…Generally, the production of soluble receptors for any particular cytokine will be produced by one of these two known mechanisms (22,23). Soluble TNF-a receptors have been reported to be produced by shedding of the cell-surface receptors (10,(32)(33)(34)(35). In vitro, TNF-a receptor shedding can be stimulated by activation of protein kinase C (pKC) (32,35).…”

Section: Introductionmentioning

confidence: 99%

“…Elevated levels of the soluble forms of TNF-R1 and TNF-R2 have been shown to inhibit TNF-a activity both in vivo and in vitro (25,(27)(28)(29)(30)(31). Furthermore, soluble TNF-R2 can act as a carrier of TNF-a when administered in vivo at relatively low concentrations, which may reflect the basal role for this soluble cytokine receptor (32).…”

Section: Introductionmentioning

confidence: 99%

“…Soluble TNF-a receptors have been reported to be produced by shedding of the cell-surface receptors (10,(32)(33)(34)(35). In vitro, TNF-a receptor shedding can be stimulated by activation of protein kinase C (pKC) (32,35).…”

Section: Introductionmentioning

confidence: 99%

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Thyrocytes Isolated from Autoimmune-Diseased Thyroids Secrete Soluble Tumor Necrosis Factor-R1 That Is Related to Their Elevated Protein Kinase C Activity

LaBue

Colburn²,

Green³

2004

Thyroid

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Soluble tumor necrosis factor (TNF)-alpha receptors have the potential to modulate TNF-alpha activity during autoimmune thyroiditis. In this study we examined cell-surface TNF-alpha receptors and soluble TNF-alpha receptor production by thyrocytes from normal and MRL-lpr(-/-) (diseased) mice, which spontaneously develop autoimmune thyroiditis. We found that murine thyrocytes possess the 55-kd receptor (TNF-R1). Examination of soluble TNF-R1 production revealed that diseased thyrocytes produced sevenfold more soluble TNF-R1 than normal thyrocytes. Furthermore, basal protein kinase C (pKC) activity in diseased thyrocytes was 67% higher than that found in normal murine thyrocytes. The elevated basal pKC activity in diseased thyrocytes was related to their enhanced production of soluble TNF-R1 because inhibition of pKC activity with calphostin C caused soluble TNF-R1 production to decrease significantly. Additionally, soluble TNF-R1 production by murine thyrocytes was not a result of cell-surface receptor shedding but through secretion of a truncated version of TNF-R1. This was evident when cell-surface TNF-R1 levels were unchanged after treatment of diseased thyrocytes with calphostin C. Also, the 28-kd form of TNF-R1, which corresponds to the soluble receptor, was present in the intracellular membranes of the diseased thyrocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Thyrocytes Isolated from Autoimmune-Diseased Thyroids Secrete Soluble Tumor Necrosis Factor-R1 That Is Related to Their Elevated Protein Kinase C Activity

LaBue

Colburn²,

Green³

2004

Thyroid

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“…Clones from the up-regulated fraction included 68 known genes, 35 expressed sequence tags, and nine novel sequences, whereas clones from the down-regulated fraction included 209 known genes, 111 expressed sequence tags, and five novel sequences. In the up-regulated fraction there were many known PMA-induced genes such as IL-8, tumor necrosis factor ␣, macrophage inflammatory protein 1, and superoxide dismutase 2 (11)(12)(13)(14). There was very little overlap between the up-and down-regulated sets of genes, except for the presence of a small number of microbeads bearing up-regulated B94 (M92357) cDNAs in the down-regulated fraction and some microbeads bearing downregulated 23 kDa basic protein transcript (X56932) in the up-regulated fraction.…”

Section: Analysis Of Differential Gene Expressionmentioning

confidence: 99%

In vitro cloning of complex mixtures of DNA on microbeads: Physical separation of differentially expressed cDNAs

Brenner¹,

Williams²,

Vermaas³

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

215

140

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We describe a method for cloning nucleic acid molecules onto the surfaces of 5-m microbeads rather than in biological hosts. A unique tag sequence is attached to each molecule, and the tagged library is amplified. Unique tagging of the molecules is achieved by sampling a small fraction (1%) of a very large repertoire of tag sequences. The resulting library is hybridized to microbeads that each carry Ϸ10 6 strands complementary to one of the tags. About 10 5 copies of each molecule are collected on each microbead. Because such clones are segregated on microbeads, they can be operated on simultaneously and then assayed separately. To demonstrate the utility of this approach, we show how to label and extract microbeads bearing clones differentially expressed between two libraries by using a fluorescence-activated cell sorter (FACS). Because no prior information about the cloned molecules is required, this process is obviously useful where sequence databases are incomplete or nonexistent. More importantly, the process also permits the isolation of clones that are expressed only in given tissues or that are differentially expressed between normal and diseased states. Such clones then may be spotted on much more cost-effective, tissue-or disease-directed, low-density planar microarrays.DNA analysis ͉ gene expression ͉ parallel cloning ͉ fluid microarray

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Association between serum levels of soluble tumor necrosis factor receptors/CA 125 and disease progression in patients with epithelial ovarian malignancy

Burger

Darcy

DiSaia

et al. 2004

Cancer

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BACKGROUND A prospective study was undertaken within the Gynecologic Oncology Group to determine whether serum levels of soluble tumor necrosis factor receptors I (sTNFR‐I) and II (sTNFR‐II), alone or in combination with CA 125, were associated with clinicopathologic characteristics or outcome in patients with epithelial ovarian malignancies. METHODS Quantitative immunoassays were performed on valid pretreatment serum specimens obtained from patients with epithelial ovarian malignancies to assess levels of sTNFR‐I, sTNFR‐II, and CA 125. The authors then analyzed the results of these immunoassays for potential correlations with clinicopathologic characteristics and outcome. RESULTS The median age of the 139 women evaluated was 59 years. Seventy‐eight percent had Stage III or IV disease, and 58% had serous carcinomas. sTNFR‐II was associated with age (P = 0.013), and CA 125 was associated with histologic subtype (P = 0.0009). In addition, sTNFR‐I (P = 0.037) and CA 125 (P < 0.0001) were associated with extent of disease. After adjusting for patient age, histologic subtype, and extent of disease, all three biomarkers were predictive of progression‐free survival, but not overall survival, when the combination was included in the model. The authors observed a 51% reduction (hazard ratio [HR], 0.49; 95% confidence interval [CI], 0.24–0.99), a 2.9‐fold increase (HR, 2.87; 95% CI, 1.15–7.20), and a 22% increase (HR, 1.22; 95% CI, 0.99–1.51) in the risk of progression for each unit increase in the log‐transformed levels of sTNFR‐I, sTNFR‐II, and CA 125, respectively. CONCLUSIONS The observations made in the current study—that among patients with low or high CA 125 levels, those with high sTNFR‐I levels and low sTNFR‐II levels had the lowest risk, that patients with low‐low or high‐high sTNFR‐I and sTNFR‐II levels, respectively, had an intermediate risk, and that patients with low sTNFR‐I levels and high sTNFR‐II levels had the highest risk of progression—suggested the potential value of simultaneous assessment of all three biomarkers in patients with epithelial ovarian malignancies. Cancer 2004. © 2004 American Cancer Society.

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The regulation of TNF receptor mRNA synthesis, membrane expression, and release by PMA- and LPS-stimulated human monocytic THP-1 cells in vitro

Cited by 29 publications

References 33 publications

Thyrocytes Isolated from Autoimmune-Diseased Thyroids Secrete Soluble Tumor Necrosis Factor-R1 That Is Related to Their Elevated Protein Kinase C Activity

Thyrocytes Isolated from Autoimmune-Diseased Thyroids Secrete Soluble Tumor Necrosis Factor-R1 That Is Related to Their Elevated Protein Kinase C Activity

In vitro cloning of complex mixtures of DNA on microbeads: Physical separation of differentially expressed cDNAs

Association between serum levels of soluble tumor necrosis factor receptors/CA 125 and disease progression in patients with epithelial ovarian malignancy

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