The regulatory effects of whey retentate from Bifidobacteria fermented milk on the microbiota of the Simulator of the Human Intestinal Microbial Ecosystem (SHIME)

Abstract

Aims: To investigate the effects of whey retentate from Bi®dobacteria fermented milk. Methods and Results: The simulator of the human intestinal microbial ecosystem (SHIME) was used. The composition of the microbiota and its metabolic activities were analysed. Changes in the microbial composition became apparent within 15 days of the treatment in the vessels representing the ileum and the large intestine. The whey retentate favoured the growth of endogenous bi®dobacteria and induced a decrease in Bacteroides fragilis and in sul®pho-reducing clostridia, especially Clostridium perfringens. After the administration was stopped, these populations tended to revert to their original levels, except for the streptococci and the staphylococci populations. The treatment also led to an increase in acetic acid, CH 4 and CO 2 production, suggesting overgrowth of some anaerobic bacteria. Ammonium, generally considered as undesirable, declined. Conclusions: The whey retentate clearly altered the microbial community in the SHIME. Signi®cance and Impact of the Study: Whey retentate appears to exert a bene®cial effect on the in vitro gastrointestinal system; these ®ndings warrant con®rmation by in vivo studies.

Beneficial effectiveness of probiotic-low-fat ice cream containing Krueo Ma Noy (Cissampelos pareira L.) gum on colon microbiome under a dynamic gut model

“…Accumulation was also greater in the distal colon vessel of the gut reactor, regardless of treatment conditions, resulting from prolonged fermentation similarly observed in the study of Chaikham and Rattanasena (2017). On the other hand, Tir Touil Meddah et al (2001) observed an overall decline in ammonium concentration during SHIME treatment with Bifidobacterium longum whey retentate. The findings of demonstrated an increase in tyramine and cadaverine concentrations following semi-continuous colon model supplementation of some specific oligosaccharides, Mäkeläinen, Ottman, Forssten et al (2010) showed that probiotic B. lactis Bi-07 supplementation alone, or in combination with polydextrose, significantly increased the production of cadaverine and spermine, while treatments of galacto-oligosaccharide showed complete suppression and reduction in the levels of specific BA in the colon simulations.…”

Arabinoxylan‐oligosaccharides (AXOS) affect the protein/carbohydrate fermentation balance and microbial population dynamics of the Simulator of Human Intestinal Microbial Ecosystem

“…For this purpose, the Simulator of Human Intestinal Microbial Ecosystem (SHIME) was used. This in vitro system has already been used for several nutrition studies (De Boever et al ., 2000; Meddah et al ., 2001; Van de Wiele et al ., 2004; 2007; Decroos et al ., 2006; Ranganathan et al ., 2006) and resulted to be a useful tool for predicting in vivo events, in terms of intestinal microbial composition and activity (Molly et al ., 1993). For instance, the SHIME results of a synbiotic treatment, using hop and Eubacterium limosum , were recently validated by Possemiers and colleagues (2006; 2008) and Bolca and colleagues (2007) using human microbiota‐associated rats and human trials.…”

Evaluation of the passage of Lactobacillus gasseri K7 and bifidobacteria from the stomach to intestines using a single reactor model

“…This is a more realistic replication of the conditions in the intestine [4]. Another system, the Simulator of the Human Intestinal Microbial Ecosystem (SHIME), consists of 5 to 6 serially connected pH controlled bioreactors [5-7]. The setup is quite complex and demands absolute anaerobic conditions.…”