The regulatory role of nerve growth factor and its receptor system in fibroblast‐like synovial cells

Abstract

Upregulation of NGF/TrkA in proinflammatory cytokine-activated FLS, the mitogenic effect of NGF on FLS, and the increased NGF level in SF of inflammatory arthritis suggest that there is cross-talk between NGF/NGF-R and FLS. These results also suggest that dysregulated production of NGF may lead to synovial cell proliferation and thus could influence the inflammatory and proliferative cascades of inflammatory arthritis.

Synovial TRPV1 is upregulated by 17-β-estradiol and involved in allodynia of inflamed temporomandibular joints in female rats

“…19,28 NGF receptor (NGF-R) (TrkA and p75) were expressed in synoviocytes (Fig. 2C), in line with the previous study, 29,30 NGF and NGF-R were unregulated in synoviocytes of patients with rheumatoid arthritis or spondyloarthritis.…”

Functional significance of nerve growth factor and its receptor (TrkA) in inflammatory arthritis

“…Furthermore, they reported that ST-derived fibroblast-like synoviocytes (FLSs) did not produce NGF in vitro . In a recent publication, we observed similarly that NGF levels in SF were significantly higher in patients with psoriatic arthritis (PsA) (365.5 ± 85.2 pg/mL) or RA (120 ± 35 pg/mL) than in patients with osteoarthritis (OA) (30 ± 6 pg/mL) [2]. However, in regard to the source of NGF, we observed that FLSs produced a significant amount of NGF.…”

Targeting bromodomain-containing protein 4 (BRD4) benefits rheumatoid arthritis

“…The overall cell growth was determined using the colorimetric MTT (3-[4,5-dimethylthiazol-2yl] diphenyltetrazolium bromide) assay [14]. Exponential phase cells were seeded in 96-well plates (5 × 10 4 cells/well) and allowed to attach overnight at 37 • C in a humidified atmosphere containing 5% CO 2 .…”