The Reitman-Frankel Colorimetric Transaminase Procedure in Suspected Myocardial Infarction

Crowley, Leonard V.

doi:10.1093/clinchem/13.6.482

Cited by 65 publications

(18 citation statements)

References 5 publications

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“…Biochemical Evaluation: Kidney serum biomarkers including creatine (Cr) mg/dL was analyzed with the Olympus 2700 analyzer (Olympus Diagnostica GmbH, Germany). In addition, blood urea nitrogen (BUN) mg/dL levels were evaluated according to Reitman-Frankel colorimetric transaminase procedure (Crowley, 1967).…”

Section: Animalsmentioning

confidence: 99%

Investigation of the effects of silymarin on valproic acid-induced kidney damage in rats

Aktaş¹,

Bayram²

2020

Harran Üniversitesi Veteriner Fakültesi Dergisi

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In this study; we aimed to investigate the protective effects of silymarin (SLY) against kidney damage on valproic acid (VPA)-induced rats using histological and biochemical evaluations. Experimental procedures were performed on 21 male Sprague Dawley rats. Rats were divided randomly into three groups: group 1, control; group 2, VPA; group 3, VPA+SLY. The control group was given 1 mL 0.9 % NaCl orally for 14 days. The VPA group was given 500 mg/kg/day VPA per os for 14 days. The VPA+SLY group was given 500 mg/kg/day VPA and 100 mg/kg/day SLY per os for 14 days. SLY treatment decreased the levels of creatinine (Cr) and blood urea nitrogen (BUN) levels significantly (p < 0.05). In addition, increased amount of thiobarbitüric acid reactive substance (TBARS) and decreased levels of glutathione (GSH) with VPA were significantly suppressed by SLY in kidney tissue (p < 0.05). Histologically, the extent of kidney damage was remarkably lower in the VPA+SLY group (p < 0.005). In addition, VPA+SLY group had decreased oxidative stress, increased antioxidant activity and decreased histopathological changes compared to VPA group. This study revealed that the kidney damage induced by VPA was attenuated with SLY administration. SLY can protect rat kidney against VPA induced damage via anti-oxidative effect and might be useful for reducing the severity of kidney injuries.

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Section: Animalsmentioning

confidence: 99%

Investigation of the effects of silymarin on valproic acid-induced kidney damage in rats

Aktaş¹,

Bayram²

2020

Harran Üniversitesi Veteriner Fakültesi Dergisi

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“…Blood samples obtained from the retro-orbital vein plexuses under ether anaesthesia were analyzed for alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), serum bilirubin, urea, creatinine, total proteins and glucose levels. ALT and AST activities in serum were measured according to Reitman-Frankel colorimetric transaminase procedure [30], whereas colorimetric determination of ALP activity was done according to the method of Belfield and Goldberg [31], using commercially available kits (BioMérieux, France).…”

Section: Biochemical Analysesmentioning

confidence: 99%

Effects of Biphenyldimethyl-dicarboxylate Administration Alone or Combined with Silymarin in the CCL₄Model of Liver Fibrosis in Rats

Abdel-Salam

Sleem

Morsy

2007

The Scientific World JOURNAL

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The effect of biphenyldimethyldicarboxylate (DDB), a synthetic compound, in use for the treatment of chronic hepatitis was studied on hepatic injury caused in rats by administration of carbon tetrachloride (CCl4). Starting at time of administration of the first dose of CCl4, rats received DDB at four dose levels (3, 15, 75 or 375 mg/kg), silymarin (22 mg/kg), a combination of DDB (75 mg/kg) and silymarin (22 mg/kg) or saline (control) once orally daily for 30 days. The administration of DDB in CCl4-treated rats at 75 or 375 mg/kg resulted in 61.2-76.2% decrease in alanine aminotransferase (ALT) and 46.9-60.8% decrease in aspartate aminotransferase (AST), respectively compared with the CCl4 control group. Silymarin treatment resulted in 34.6 and 30% decrease in ALT and AST, while DDB (75 mg/kg) combined with silymarin (22 mg/kg) resulted in 58.2 and 31% decrease in ALT and AST, respectively. Serum creatinine increased by 50% by DDB at 375 mg/kg. After treatment with DDB at 75 or 375 mg/kg or DDB combined with silymarin, the development of liver necrosis and fibrosis caused by CCl4 was markedly reduced, while after DDB combined with silymarin no DNA aneuploid cells could be observed. The decrease in glycogen and protein contents in hepatocytes caused by CCl4 was markedly prevented by co-treatment with DDB at 75 or 375 mg/kg or DDB combined with silymarin. It is concluded that in the model of hepatic injury caused by chronic administration of CCl4 in rats, the synthetic compound DDB, limits hepatocellular injury and exerts antifibrotic effect. Better improvement in protein, DNA, mucopolysaccharide content was seen after both DDB and silymarin compared to DDB alone. It is suggested, therefore, that DDB alone or in combination with silymarin might prove of benefit in the therapy of chronic liver disease. Monitoring of kidney functions in patients taking DDB is warranted.

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“…The activities of serum AST and ALT were determined by the Reitman-Frankel method with slight modifications. [ 11 ] Hydroxyproline content in liver hydrolysate was determined by the slightly modified method of Reddy and Enwemek. [ 12 ] Briefly, the lyophilized hepatic tissue (10 mg) was powdered and hydrolyzed with 6 N HCl for 60 min at 100°C.…”

Section: Methodsmentioning

confidence: 99%

The ethanolic extract of Juglans sinensis leaves and twigs attenuates CCl₄-induced hepatic oxidative stress in rats

2015

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Background:The nuts of Juglans sinensis Dode, walnut tree, are rich in unsaturated fatty acids and bioactive compounds with antioxidant activity on liver damages. However, hepatoprotective activity of the leaves and twigs of J. sinensis have not intensively studied yet.Objective:Hepatoprotective activity of the refined ethanolic extract of J. sinensis (JSE3) was evaluated using carbon tetrachloride (CCl4)-intoxicated rats.Materials and Methods:Hepatotoxicity was induced in Sprague Dawley rats by intraperitoneal injection of CCl4 for 6 weeks in the presence or absence of JSE3 (100 and 200 mg/kg body weight). The hepatoprotective activity of JSE3 was assessed by biochemical parameters including plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), and antioxidant enzymes, such as superoxide dismutase (SOD), glutathione reductase, glutathione peroxide, reduced glutathione and oxidized glutathione, along with histopathological studies on hepatic tissue.Results:JSE3 significantly decreased the elevated levels of AST and ALT and restored the reduced levels of antioxidant enzymes. JSE3 also decreased the amounts of collagen content accumulated by CCl4 intoxication.Conclusion:These results suggested that the refined extract of J. sinensis may have a potential to be developed as a therapeutic agent to treat hepatic diseases, such as fatty liver and hepatic fibrosis.

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The Reitman-Frankel Colorimetric Transaminase Procedure in Suspected Myocardial Infarction

Cited by 65 publications

References 5 publications

Investigation of the effects of silymarin on valproic acid-induced kidney damage in rats

Investigation of the effects of silymarin on valproic acid-induced kidney damage in rats

Effects of Biphenyldimethyl-dicarboxylate Administration Alone or Combined with Silymarin in the CCL₄Model of Liver Fibrosis in Rats

The ethanolic extract of Juglans sinensis leaves and twigs attenuates CCl₄-induced hepatic oxidative stress in rats

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The Reitman-Frankel Colorimetric Transaminase Procedure in Suspected Myocardial Infarction

Cited by 65 publications

References 5 publications

Investigation of the effects of silymarin on valproic acid-induced kidney damage in rats

Investigation of the effects of silymarin on valproic acid-induced kidney damage in rats

Effects of Biphenyldimethyl-dicarboxylate Administration Alone or Combined with Silymarin in the CCL4Model of Liver Fibrosis in Rats

The ethanolic extract of Juglans sinensis leaves and twigs attenuates CCl4-induced hepatic oxidative stress in rats

Contact Info

Product

Resources

About

Effects of Biphenyldimethyl-dicarboxylate Administration Alone or Combined with Silymarin in the CCL₄Model of Liver Fibrosis in Rats

The ethanolic extract of Juglans sinensis leaves and twigs attenuates CCl₄-induced hepatic oxidative stress in rats